Oleanolic acid and ursolic acid in the preparation of modulating vitamin D 3 Use in drugs that metabolize enzyme activity
A technology of oleanolic acid and ursolic acid, applied in the field of medicine, can solve problems such as poor water solubility, high toxicity, and fast metabolism
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Embodiment 1
[0055] Example 1 Cell experiment
[0056] (1) Regulatory effect of mixing different proportions of oleanolic acid (OA) and ursolic acid (UA) on the activity of CYP27B1 promoter:
[0057]HKC-8 cells were used in the experiment (provided by the laboratory of Prof. Racusen of the Department of Pathology of Johns Hopkins University in the United States, the references are as follows: Racusen LC, Monteil C, Sgrignoli A, Lucskay M, Marouillat S, Rhim JG, et al. (1997). Cell lines withextended in vitro growth potential from human renal proximal tubule:characterization, response to inducers, and comparison with established celllines. Journal of Laboratory and Clinical Medicine 129:318-329.), using the inserted CYP27B1 transcription promoter sequence The pGL-3-basic vector (pGL-3-basic vector was provided by the laboratory of Dr. Farzana Perwad, a professor at the University of California San Francisco in the United States: References are as follows: Chanakul A, Zhang MY, Louw A, Armbr...
Embodiment 2
[0062] Example 2 Animal experiment verification (menopausal osteoporosis model)
[0063] In this example, the optimal ratio of oleanolic acid and ursolic acid was mixed (oleanolic acid: ursolic acid = 3:1), fed to 5-month-old ovariectomized female rats, and after 6 weeks of feeding, the in vivo Serum 1,25(OH) 2 D. 3 Level, intestinal calcium absorption and calcium balance, bone density and other indicators, to verify the optimal ratio of oleanolic acid and ursolic acid mixed (oleanolic acid: ursolic acid = 3:1) to regulate vitamin D in the body 3 metabolism, and to improve calcium balance and bone density.
[0064] 1. Materials and methods
[0065] 1.1 Animals and materials
[0066] Animals: 20 5-month-old SPF female ovariectomized SD rats (average body weight 250 g), purchased from the Experimental Animal Center of Purdue University, USA.
[0067] 1.2 Experimental method
[0068] 1.2.1 Experimental grouping and drug treatment: After the animals adapted to the environmen...
Embodiment 3
[0075] Example 3 Animal experiment verification (senile osteoporosis model)
[0076] In this example, the optimal ratio of oleanolic acid and ursolic acid was mixed (oleanolic acid: ursolic acid = 3:1), fed to 11-month-old female rats, and after 12 weeks of feeding, the serum in vivo was detected 1,25(OH) 2 D. 3 Level and serum parathyroid hormone (PTH) level, intestinal calcium absorption and calcium balance, bone density and other indicators, verify the optimal ratio of oleanolic acid and ursolic acid mixed (oleanolic acid: ursolic acid = 3: 1) Regulate vitamin D metabolism in the body, and improve calcium balance and bone density.
[0077] 1. Materials and methods
[0078] 1.1 Animals and materials
[0079] Animals: 24 11-month-old SPF female SD rats (average body weight 389g), and 8 3-month-old SPF female SD rats (average body weight 220g) as a control group were purchased from Guangdong Medical Experimental Animal Center ( Animal certificate number: SCXK (Guangdong) ...
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