Specific primer pair for identifying tangerine fruit flies and citrus fruit flies and application thereof

Abstract

The invention discloses a specific primer pair for identifying tangerine fruit flies and citrus fruit flies and an application thereof. The invention designs a specific primer pair for identifying thetangerine fruit flies and the citrus fruit flies, and develops a molecular identification technology based on conventional PCR. Experiments prove that the molecular identification technology based onthe conventional PCR can achieve the species identification of the tangerine fruit flies and the citrus fruit flies, and has the advantages of strong specificity, high sensitivity, low cost, simple operation and short time consumption. The primer pair not only solves the problem of identifying the species of non-adult fruit flies and residues of the adult fruit flies, but also provides a practical technology and a theoretical basis for monitoring the epidemic situation of the tangerine fruit flies and citrus fruit flies, distributing and invading of the tangerine fruit flies and the citrus fruit flies in main production areas of China, and subsequent plant quarantine and field management. The primer pair is of great significance to effectively prevent further invasion and diffusion of thetangerine fruit flies and citrus fruit flies, protect agricultural production and ecological security, and promote the development of economic and trade in China.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a pair of specific primers for identifying Bactrocera citrus and Bactrocera citrus and application thereof. Background technique [0002] Tephritids belong to Diptera Tephritidae and are widely distributed all over the world. There are about 500 genera and 4,500 species, of which 26 genera and 223 species are economically significant. Fruit flies have a wide range of hosts. While doing damage to economic crops such as fruits and vegetables, they also seriously affect the foreign trade of fruits and vegetables, causing trade barriers and causing huge economic losses. Bactroceratsuneonis and B.minax are two species of oligophagous fruit flies of the subgenus Tetradacus of the genus Bactrocera Bactrocera, which are only harmful to sweet oranges, limes, ponkans, and Wenzhou honey. Citrus crops such as orange, lemon, citron, bergamot, kumquat of the genus Fortunella, ...

AI Technical Summary

Problems solved by technology

The traditional identification of fruit flies mainly relies on morphological characteristics, and the external morphological characteristics of the two species of Bactrocera are very similar, which can only be determined by the mane sequence of the adult mesothorax (Bactrocera dorsalis has a pair of shoulder plate bristles, and Absence of bristles; Bactrocera citrus has 2 pairs of scapular bristles and 1-2 pairs of upper bristles on forewings) and female ovipositor characteristics (female ovipositor of Bactrocera citrus is longer, as long as second The sum of the lengths of -5 ventral and back plates is nearly the same, and the end is slightly pointed; the basal segment of the ovipositor of the female citrus fruit fly is shorter, about the sum of the lengths of the 4th and 5th ventral and back plates, and the end is trilobate) as identification basis, This has caused great difficulties in the morphological identification of the two species of Bactrocera

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