A kind of compound plant extract, preparation method and application
A plant extract, extract technology, applied in the fields of application, food science, fruit and vegetable preservation, etc., to protect the environment, prolong the storage period, and ensure the effect of human health
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Embodiment 1
[0020] Antibacterial test of compound plant extracts:
[0021] (1) Experimental bacteria (bacteria name in italics)
[0022] Foodborne pathogens:
[0023] Pseudomonas fluorescens (Pseudomonadaceae Fluorescens ATCC 13525);
[0024] Salmonella enteritidis (Salmonella enteritidis CMCC(B)50335);
[0025] Proteus (proteus species CMCC(B)49027);
[0026] Escherichia coli (E.coli k-12IFO 3301);
[0027] Listeria monocytogenes ATCC 19115;
[0028] Staphylococcus aureus (Staphylococcus aureus CMCC(B)26003)
[0029] (2) Experimental method
[0030] 2.1 Compound plant extracts
[0031] Prepare four plants: clove, rosemary, licorice, and oregano. After the prepared cloves, rosemary, licorice, and oregano are crushed, they are respectively soaked and extracted with 99.5% absolute ethanol. The quality of each plant is comparable to that of ethanol The volume ratio of each is 1:9 (m / v), soaking and shaking for 40-52h, preferably, under the condition of 130-170rpm / min, soaking and sha...
Embodiment 2
[0052] Antibacterial test of single plant extracts:
[0053] (1) Experimental bacteria (bacteria name in italics)
[0054] Foodborne pathogens:
[0055] Pseudomonas fluorescens (Pseudomonadaceae Fluorescens ATCC 13525);
[0056] Salmonella enteritidis (Salmonella enteritidis CMCC(B)50335);
[0057] Proteus (proteus species CMCC(B)49027);
[0058] Escherichia coli (E.coli k-12 IFO 3301);
[0059] Listeria monocytogenes ATCC 19115;
[0060] Staphylococcus aureus (Staphylococcus aureus CMCC(B)26003)
[0061] (2) Experimental method
[0062] 2.1 Single plant extract
[0063] Prepare eight kinds of plants including cinnamon, clove, sage, rosemary, licorice, thyme, star anise and oregano After being pulverized, soak and extract with 99.5% absolute ethanol respectively. The ratio of the mass of each plant to the volume of ethanol is 1:9 (m / v). Under the condition of 150rpm / min, soak and shake for 48h, then filter Obtain the filtrate (the filtrate can be stored at 4°C), there...
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