Application of scillaren compound to preparation of flavivirus infection resistant medicament
A kind of compound, the technology of onionin, applied in the field of medicine, can solve the problem of not found anti-flavivirus and other problems
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Embodiment 1
[0047] Embodiment 1. compound anti-flavivirus activity detection method
[0048] 1.1. Preparation of the test compound solution: the test compound was dissolved in DMSO as a solvent, the final concentration of the DMSO medium was 0.1%, and the final concentration of the test compound: 100nM, 30nM, 10nM, 3nM, 1nM.
[0049] 1.2. Anti-Zika virus activity detection method of compounds
[0050] The day before infection, the cells were inoculated with 2.0×10 4The density per well was inoculated into a 96-well plate, and the test compound was added, with DMSO as the control. After 1 h, the original culture medium was discarded, and Zika virus (GZ01 / 2016 strain, FSS13025 / 2010 strain and SZ01 / 2016 strain) culture medium (MOI: 0.5) was added, and the test compound was added at the same time. 24 hours after infection, the virus liquid was discarded, the cells were fixed with 4% paraformaldehyde solution, and PBS containing 0.1% Triton X-100 was added, and PBS containing 1% goat serum a...
Embodiment 2
[0077] Example 2. Insaridin A anti-flavivirus activity (Table 5)
[0078] 2.1. Anti-Zika virus activity of insaridin A
[0079]
[0080] The experimental results showed that insaridin A has anti-Zika virus activity. In this experiment, Zika virus GZ01 / 2016 strain and two other Zika virus strains (FSS13025 / 2010, SZ01 / 2016) were used, and it was found that instriside A had an inhibitory effect on them, and the half inhibitory concentration (IC 50 ) were 5.6nM, 4.9nM, 4.7nM, respectively.
[0081] 2.2. Compound anti-dengue virus activity
[0082] The experimental results showed that instritin A had anti-dengue virus activity, and the half maximal inhibitory concentration (IC 50 ) is 3.3nM.
[0083] 2.3. Compound anti-yellow fever virus activity
[0084] The experimental results showed that instritin A had anti-yellow fever virus activity, and the half inhibitory concentration (IC 50 ) is 3.4nM.
[0085] 2.4. Anti-Japanese Encephalitis Virus Activity of Compounds
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