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Preparation method of Dieffenbachia polysaccharide and its application in the preparation of anti-intestinal tumor drugs or in promoting the growth of probiotics

A white-flowered tiger eye evergreen and evergreen technology is applied in the direction of anti-tumor drugs, drug combinations, and medical preparations containing active ingredients. Promote the growth of probiotics and inhibit the effect of cancer cells

Active Publication Date: 2021-01-05
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the anti-tumor effect of the polysaccharides of tiger eye evergreen, especially in the aspect of anti-intestinal tumors

Method used

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  • Preparation method of Dieffenbachia polysaccharide and its application in the preparation of anti-intestinal tumor drugs or in promoting the growth of probiotics
  • Preparation method of Dieffenbachia polysaccharide and its application in the preparation of anti-intestinal tumor drugs or in promoting the growth of probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of the polysaccharide of evergreen diphtheria chinensis, the steps comprising:

[0026] (1) the Dieffenbachia vinifera corm that has been cultivated for three years is dried and pulverized;

[0027] (2) The dry powder obtained in step (1) is used as a solvent in distilled water, and subjected to Soxhlet extraction at 90°C to 95°C for 3 to 5 hours to obtain an extract;

[0028] (3) Concentrate the extract obtained in step (2) by rotary evaporation, and the conditions of rotary evaporation are controlled at 70°C±5°C, 80~100rpm, 0.09~0.10MPa; concentrate until the liquid volume is 1 / 4-1 / 6 o'clock recovery solution;

[0029] (4) The concentrated extract obtained in step (3) is pre-frozen and solidified at -15--25°C, and then freeze-dried at -70--80°C to make a dry powder;

[0030] (5) the dry powder that step (4) is obtained is dissolved with distilled water, and the upper dextran gel G-50 chromatographic column of dissolving solution;

[0031] (6...

Embodiment 2

[0034] Inhibitory effect of Dieffenbachia polysaccharide on human rectal cancer cell HR-8348

[0035] Take a bottle of human rectal cancer cell HR-8348 that is in exponential growth phase and in good condition, and make a suspension; count the cells, and adjust the number of cells in the cell suspension to 1×10 5 / mL; inoculated on a 96-well plate, 100 μL / well, cultured for 12 hours; discarded the culture medium, added Dieffenbachia polysaccharide, the final concentration of the polysaccharide was 1 μg / ml, 0.5 μg / ml, 0.25 μg / ml, 0.1 μg / ml, 0.05μg / ml, 0.025μg / ml, 0μg / ml, cultivate for 48 hours; discard the culture medium, add CCK-8 reagent, 10μL / well, cultivate for 4 hours, measure A450nm, and calculate the cell inhibition rate.

[0036] Cell inhibition rate (%)=[1-(A experimental group-A growth control group) / (A growth control group-A blank)]×100%

[0037] Experimental result: 0.025~1.00μg·mL -1 Dieffenbachia polysaccharide has inhibitory effect on human rectal cancer cell ...

Embodiment 3

[0041] Inhibitory effect of Dieffenbachia polysaccharides on human colon cancer cells SW-480

[0042] Take a bottle of human colon cancer cell SW-480 in a good state in the exponential growth phase, and make a suspension; count the cells, and adjust the number of cells in the cell suspension to 1×10 5 / mL; inoculated on a 96-well plate, 100 μL / well, cultured for 12 hours; discarded the culture medium, added Dieffenbachia polysaccharide, the final concentration of the polysaccharide was 1 μg / ml, 0.5 μg / ml, 0.25 μg / ml, 0.1 μg / ml, 0.05μg / ml, 0.025μg / ml, 0μg / ml, cultivate for 48 hours; discard the culture medium, add CCK-8 reagent, 10μL / well, cultivate for 4 hours, measure A450nm, and calculate the cell inhibition rate.

[0043] Cell inhibition rate (%)=[1-(A experimental group-A growth control group) / (A growth control group-A blank)]×100%

[0044] Experimental result: 0.025~1.00μg·mL -1 Dieffenbachia polysaccharides have inhibitory effect on human colon cancer cell SW-480. Th...

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Abstract

The invention discloses a preparation method of ornithogalum thyrsoide polysaccharide, and application of the ornithogalum thyrsoide polysaccharide to preparation of medicines for treating intestinaltumors or to promotion of growth of probiotics. Polysaccharide in corms of ornithogalum thyrsoide which has been cultured for three years is extracted through a Soxhlet extraction method, and dry powder is obtained through a two-step lyophilization method. Through pre-freezing, it can be guaranteed that a polysaccharide structure in an extract is fixed, and modification of polysaccharide caused bya heating-drying mode is prevented. It is found that the ornithogalum thyrsoide polysaccharide can effectively restrain human colon carcinoma cells and rectal cancer cells and can promote growth of intestinal probiotics, when the ornithogalum thyrsoide polysaccharide is used as a medicine for resisting intestinal tumors, the ornithogalum thyrsoide polysaccharide not only can restrain cancer cellsbut also can promote growth of probiotics, and intestinal tumors can be better treated through the synergistic effect.

Description

technical field [0001] The present invention relates to a preparation method of Dieffenbachia polysaccharide and its application in preparing anti-intestinal tumor drugs or promoting the growth of probiotics. Background technique [0002] Ornithogalum thyrsoide, also known as pearl grass, squill, bird's milk flower, gourd orchid, etc., belongs to the genus Ornithogalum thyrsoide in the family Liliaceae, and is an evergreen perennial herb. The medicine is slightly cold, sweet in taste, and returns to the liver and spleen meridians. Folks use its whole herb or bulbs as medicine. The stems and leaves are smashed and applied externally on the affected parts of unknown swelling or boils, sores, carbuncles, furuncles, etc., which can clear away heat, reduce inflammation, reduce swelling and relieve pain; decoction orally can clear away heat and detoxify, soften hardness and remove blood stasis. It is mostly used to treat hepatitis, Cholecystitis, liver cirrhosis and other diseas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P1/00
Inventor 杜季梅邓拓叶盛郑亦胡
Owner WENZHOU MEDICAL UNIV
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