High-performance liquid chromatography method for metronidazole and norfloxacin in toothpaste

A high-performance liquid chromatography and norfloxacin technology, which is applied in the field of high-performance liquid chromatography analysis of metronidazole and norfloxacin in toothpaste, can solve the problem that metronidazole and norfloxacin have no relevant analytical methods, etc. problem, to achieve the effect of reliable, accurate quantitative analysis and accurate test results.

Inactive Publication Date: 2019-05-28
JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
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  • Claims
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Problems solved by technology

At present, there is no relevant analytical method for the detection of metronidazole and norfloxacin in the matrix of toothpaste in domestic and foreign literature records.

Method used

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  • High-performance liquid chromatography method for metronidazole and norfloxacin in toothpaste
  • High-performance liquid chromatography method for metronidazole and norfloxacin in toothpaste
  • High-performance liquid chromatography method for metronidazole and norfloxacin in toothpaste

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Embodiment 1

[0060] Standard working solution preparation:

[0061] The above-mentioned 100mg / L mixed standard stock solution was diluted step by step to configure 50, 20, 10, 5mg / L standard working solutions. Filter through a 0.45 μm membrane filter for later use.

[0062] Sample preparation:

[0063] After the toothpaste sample is squeezed out about 20mm, accurately weigh 5.0000g (accurate to 0.0002g) of a uniform sample in a colorimetric tube (25mL), add the extract [0.1% (v / v) formic acid solution: acetonitrile (95:5, v / v)] to the scale line, vortex for 2 minutes, sonicate in a water bath below 25°C for 10 minutes, transfer the supernatant to a centrifuge tube after standing still, centrifuge at 4000r / min for 10 minutes, take the supernatant and pass through a 0.45um micropore Inject the sample after the membrane filter.

[0064] Blank sample preparation:

[0065] Except that no sample was added, all the treatments were carried out according to the treatment steps of sample prepara...

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Abstract

The invention discloses a high-performance liquid chromatography method for metronidazole and norfloxacin in toothpaste, and belongs to the technical field of analysis. The method comprises the step that high-performance liquid chromatography is adopted for detecting a blank sample, a sample solution and a standard curve solution separately, wherein as for the detection sequence, the blank sample,a standard curve, a sample, a sample and a standard curve are detected in sequence, what needs to be paid attention to is that the sample adding frequency of the samples between the two standard curves should not exceed 15, and if the frequency exceeds 15, a quality control sample or a standard curve needs to be added in the middle. By means of the method, the content of the metronidazole and norfloxacin in the toothpaste samples can be quantitatively analyzed quickly and accurately, and the method can be used for monitoring the quality of commercially-available toothpaste products by marketregulatory authorities and has the advantages of being accurate in detection result and reliable.

Description

technical field [0001] The invention relates to the technical field of component analysis, in particular to a high performance liquid chromatography analysis method for metronidazole and norfloxacin in toothpaste. Background technique [0002] Metronidazole and norfloxacin are two common antibiotics. [0003] The chemical name of metronidazole is: 1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole, and the molecular formula is C 6 h 9 N 3 o 3 , the molecular weight is 171.154, and the structural formula is as follows: [0004] [0005] The chemical name of norfloxacin is: 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, the molecular formula is C 16 h 18 FN 3 o 3 , the molecular weight is 319.331, and the structural formula is as follows: [0006] [0007] The national standard GB 22115-2008 "Standards for Raw Materials for Toothpaste" has listed antibiotics as substances that are prohibited from being added to toothpaste, and metroni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 洪华王莉潘虹朱利利孙长恩
Owner JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
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