Method and device for continuously obtaining plant secondary metabolic products through tissue culture

A technology of secondary metabolites and tissue culture, which is applied in the field of plant biology, can solve the problems of uniform irrigation of plant tissues and organs, the influence of plant tissues and organs growth, and high difficulty in operation, so as to reduce manpower consumption, facilitate collection and place difficulty. Effect

Inactive Publication Date: 2019-05-31
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in actual use, there are still some shortcomings. For example, in actual implementation, plant tissues and organs need to be placed on a multi-layer culture grid. Placement, the actual operation is difficult, and when it is used in indust

Method used

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  • Method and device for continuously obtaining plant secondary metabolic products through tissue culture
  • Method and device for continuously obtaining plant secondary metabolic products through tissue culture
  • Method and device for continuously obtaining plant secondary metabolic products through tissue culture

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Example Embodiment

[0039] The specific implementation is as follows: the winding roller 21 and the positive and negative motors 22 are provided to facilitate the use of the positive and negative motors 22 to drive the winding roller 21 to rotate, thereby driving the suspension rope 20 outside the winding roller 21 to descend, rise or The descending suspension rope 20 drives the nozzle 13 up or down through the suspension ring 19, the longitudinal connecting plate 18 and the hollow column 12, so as to adjust the distance between the culture bucket 8 and the nozzle 13 so as to be suitable for various plant tissues and organs. to cultivate.

[0040] As attached figure 1 , Attached Figure 5 And Image 6 The shown method and device for continuously obtaining plant secondary metabolites through tissue culture further includes a sampling mechanism 28, which is arranged on the other side of the culture solution storage bucket 24, and the sampling mechanism 28 includes a sampling tube 29. The sampling tub...

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Abstract

The invention discloses a method and device for continuously obtaining plant secondary metabolic products through tissue culture, and particularly relates to the field of plant biology. The device comprises a box shell. A detachable net frame mechanism and a precise dosage mechanism are arranged in the box shell. The precise dosage mechanism is arranged on the top of the detachable net frame mechanism. The detachable net frame mechanism comprises an annular fixing plate. A filter screen is fixedly arranged on the inner side of the annular fixing plate. A plurality of placement holes run through the filter screen. A bearing hopper is arranged inside each placement hole. A culture hopper is arranged inside each bearing hopper. A plurality of through holes run through the bearing hoppers andthe culture hoppers. Placed plant tissue and organs can be in a scattered state; compared with the prior art, the placement difficulty is low, the placement effect is precise, there is no need to makeadjustment multiple times, labor consumption is effectively reduced, meanwhile it can be ensured that the plant tissue and organs in the culture hoppers can be effectively watered, and the good growth of the plant tissue and organs is ensured.

Description

technical field [0001] The invention relates to the field of plant biotechnology, more specifically, the invention relates to a method and a device for continuously obtaining plant secondary metabolites through tissue culture. Background technique [0002] Secondary metabolites are non-essential small-molecule organic compounds produced by secondary metabolism in a class of cell life activities or in the normal operation of plant growth and development, and their production and distribution are usually specific to species, organs, tissues, and growth and development stages , can be divided into seven categories: phenylpropanoids, quinones, flavonoids, tannins, terpenes, steroids and their glycosides, and alkaloids. Secondary metabolites in medicinal plants are the key to their medicinal effects Element. [0003] The invention patent of patent application publication number CN103120124B discloses a method for continuously obtaining plant secondary metabolites through tissue ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘国花唐宁李哲馨
Owner CHONGQING UNIV OF ARTS & SCI
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