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Adipose tissue for tissue transplantation and preparation method thereof

A fat tissue and tissue transplantation technology, applied in bone/connective tissue cells, medical science, prosthesis, etc., can solve problems such as damage to healthy tissue, damage, etc., to improve the success rate, improve permeability, improve speed and quality. Effect

Inactive Publication Date: 2019-06-07
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional solution is autologous tissue transplantation. Although satisfactory curative effect can be obtained, it is at the expense of autologous healthy tissue, which will cause many complications and additional damage while damaging the original healthy tissue.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] An adipose tissue for tissue transplantation, comprising a biological scaffold, mesenchymal stem cells and cell growth promoting substances, the number of the mesenchymal stem cells inoculated in each gram of the biological scaffold is 10 6 ~10 7 The biological scaffold includes polyorthoester and poly(lactic-co-glycolic acid) with a ratio of parts by weight of 1:1; the cell growth-promoting substance includes Centella asiatica with a ratio of parts by weight of 5:4:1 glycosides, creatine, and allantoin.

Embodiment 2

[0045] An adipose tissue for tissue transplantation, comprising a biological scaffold, mesenchymal stem cells and cell growth promoting substances, the number of the mesenchymal stem cells inoculated in each gram of the biological scaffold is 10 6 ~10 7 The biological scaffold includes polyorthoester and polylactic acid-glycolic acid copolymer with a ratio of parts by weight of 1:5; the cell growth-promoting substance includes Centella asiatica with a ratio of parts by weight of 5:2:1 glycosides, creatine, and allantoin.

Embodiment 3

[0047] A method for preparing adipose tissue for tissue transplantation, comprising the steps of:

[0048] S1: extracting mesenchymal stem cells, primary culture for 2-3 days, and subculture the obtained cells for 1-2 weeks;

[0049] S2: Mix the cells obtained in step S1 with the biological scaffold in proportion, add the pre-sonicated induction medium, shake and mix gently, and culture in a microgravity rotary generator for 1 to 2 weeks;

[0050] S3: Centrifuge the culture obtained in step S2 at low speed, discard the supernatant, and add cell growth promoting substances to obtain the adipose tissue.

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Abstract

The invention discloses adipose tissue for tissue transplantation and a preparation method thereof. Seed sells are inoculated into a biological scaffold to form a spatial structure to prevent the adipose tissue from liquefaction after being inoculated into a body. Meanwhile, cell growth promoting substances are added to the proliferation and renewal of cells after the adipose tissue is inoculatedinto the body, thereby accelerating tissue renewal and wound healing. Cell growth factors are added to a subculture medium, which can effectively promote the division and renewal of mesenchymal stem cells and improve the speed and quality of culture. In order to ensure that an induction medium and a cell growth promoting substance solution can sufficiently penetrate into cell gaps, ultrasonic treatment is carried out in advance to disperse liquid into small droplets and improve the permeability of the liquid. In this way, the rate of the adipose tissue culture and the survival rate after the transplantation can be improved, thereby improving the success rate of the adipose tissue transplantation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, in particular to an adipose tissue for tissue transplantation and a preparation method thereof. Background technique [0002] Tissue transplantation is a technique of transplanting autologous tissue or artificial materials to a certain part of the body to restore deformities or tissue defects caused by congenital or acquired factors. Currently common tissue transplantation includes skin transplantation, adipose tissue transplantation, bone tissue transplantation, etc. Among them, adipose tissue transplantation has an important aesthetic effect in addition to the therapeutic effect for breast cancer patients. The traditional solution is autologous tissue transplantation. Although satisfactory curative effect can be obtained, it is at the expense of autologous healthy tissue, which will cause many complications and additional damage while damaging the original healthy tissue. Therefore,...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/38A61L27/54C12N5/0775
Inventor 董明珠李霞云潘新刘世红卢家堃
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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