Preparation method of quantum dot-based nano-magnetic particles and application of quantum dot-based nano-magnetic particles to multivariant foodborne pathogenic bacterium detection

A nano-magnetic particle, nano-particle technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problem of time-consuming and other problems, and achieve the effect of reducing investment costs, food safety assurance, and rapid detection

Inactive Publication Date: 2019-06-07
FUYANG NORMAL UNIVERSITY
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Problems solved by technology

[0004] Traditional detection methods for food-borne pathogens mainly include plate separation method, chemical analysis method, molecular biology method and immunological detection method. For bacteria enrichment, separation, biochemical test and serological identification, etc., although the result is stable, the method takes too long

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  • Preparation method of quantum dot-based nano-magnetic particles and application of quantum dot-based nano-magnetic particles to multivariant foodborne pathogenic bacterium detection

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[0026] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the examples in the implementation manners, other examples obtained by those skilled in the art without making creative efforts all belong to the protection scope of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0027] Describe specific embodiments of the present invention below in conjunction with accompanying drawing

[0028] The preparation method of the nano magnetic particle based on quantum dots, comprises the following ste...

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Abstract

The invention belongs to the field of functional material and pathogen detection and relates to a preparation method of quantum dot-based nano-magnetic particles and application of the quantum dot-based nano-magnetic particles to multivariant foodborne pathogenic bacterium detection. The preparation method includes the following steps that: S1, nano magnetic particles are synthesized; S2, functionalized nano magnetic particles are prepared; S3, multicolor quantum dot nanoparticles are prepared; S4, fluorescent labeled molecules are prepared; and S5, the functionalized nano magnetic particles and the fluorescent labeled molecules are coupled. With the quantum dot-based nano-magnetic particles of the invention, the simultaneous and rapid detection of multivariant foodborne pathogenic bacteria in food samples can be realized, operation is simple and convenient, the actual test consumption of a detection mechanism is greatly saved, and investment cost can be reduced. The simple and economical food hazard detecting means will attract more capital into the food testing industry and promote the food testing industry, and especially the rapid development of pathogen detection.

Description

technical field [0001] The invention relates to the field of functional materials and pathogenic bacteria detection, the preparation of nano magnetic particles based on quantum dots and its application in the detection of multiple food-borne pathogenic bacteria. Background technique [0002] Along with the endless food safety problems, ensuring food safety is a major issue of common concern in the current society. Food hazards can be roughly divided into three categories: physical hazards, biological hazards, and chemical hazards. Biological hazards include food-borne pathogens, biotoxins, food-borne viruses, and food allergens. Wait. Among them, food-borne pathogens are common pathogenic microorganisms, which refer to bacteria that originate from food and can cause human diseases after infection. Mainly including Escherichia coli, Salmonella, Staphylococcus aureus and so on. [0003] When food is processed, stored and transported, it is extremely susceptible to contamina...

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Application Information

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IPC IPC(8): G01N33/543G01N33/569G01N21/64
Inventor 王旗夏娟张朋刘冬冬
Owner FUYANG NORMAL UNIVERSITY
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