Reagent kit for detecting cardiovascular disease medication genes and detection method

A technology for detecting reagents and kits, which is applied in the field of kits for detecting drug genes for cardiovascular diseases, can solve the problems of low detection throughput, few detection sites, long detection cycle, etc., and achieve strong detection specificity and high sensitivity Effect

Active Publication Date: 2019-06-11
ZHEJIANG DIGENA DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection methods have defects of various degrees, such as cumbersome operation process, poor repeatability, difficult interpretation of results, few detection sites, low detection throughput, long detection cycle, high detection cost, etc.

Method used

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  • Reagent kit for detecting cardiovascular disease medication genes and detection method
  • Reagent kit for detecting cardiovascular disease medication genes and detection method
  • Reagent kit for detecting cardiovascular disease medication genes and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1. Interpretation related to detection of warfarin medication

[0068] Open the Typer4.0 software to analyze the sites related to warfarin medication, such as image 3 , and the interpretation results are shown in Table 3.

[0069] Table 3. Interpretation of site results related to warfarin medication

[0070]

[0071] Table 4. Analysis of loci related to warfarin medication

[0072]

[0073] Recommended initial dose of warfarin according to the combined genotype of VKORC1 and CYP2C9 (mg) 1

[0074] Warfarin stable dose D (mg / day) = [1.432+0.338×(VKORC1-1639AG)+0.579×(VKORC1-1639GG)–0.263×(CYP2C9*1*3)–0.852×(CYP2C9*3*3) -0.004 Age+0.264×BSA+0.057×AVR+0.065×Sex+0.085×Smoking habit+0.057×Atrial fibrillation+0.132×Aspirin-0.0592× Amiodarone]

[0075] Note: VKORC1-1639AG means that the patient has the -1639AG genotype and takes a value of 1, and the -1639AA or -1639GG genotype takes a value of 0; VKORC1-1639GG means that the patient has a -1639GG genotype...

Embodiment 2

[0077] Example 2. Interpretation of loci related to drug metabolism in hypertension

[0078] Use Typer 4.0 software to analyze the loci of genes related to drug metabolism in hypertension, such as Figure 4 , and the interpretation results are shown in Table 5.

[0079] Table 5. Interpretation of genes related to drug metabolism in hypertension

[0080]

[0081]

Embodiment 3

[0082] Example 3. Locus analysis of genes related to statin drug metabolism

[0083] Use Typer 4.0 software to analyze the loci of genes related to statin drug metabolism, such as Figure 5 , the results are shown in Table 6.

[0084] Table 6. Interpretation of the results of loci related to statin drug metabolism

[0085]

[0086]

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Abstract

The invention belongs to the technical field of gene detection, and relates to a reagent kit for detecting cardiovascular disease medication genes and a detection method. The reagent kit comprises a detection reagent for 17 mutant sites of 11 genes, in accordance with exceptional gene variation sites of the 11 genes, an amplification primer and a single-basic-group extension primer of each site are designed, through multiplex PCR and a multiple single-basic-group extension reaction, mass spectrometric analysis is performed on a specific product, and the genotype of each site is judged. The detection specificity is high, the sensitivity is high, and the reagent kit can be applied to scientific research, medicine treatment effect research, cardiovascular disease relevant investigation and cardiovascular system clinical medication guidance.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and relates to a kit for detecting cardiovascular disease drug genes, in particular to the detection of 17 mutation sites of 11 genes related to cardiovascular disease drug in human samples. For the abnormal gene variation sites in eleven genes, design amplification primers and single-base extension primers for each site, and then conduct mass spectrometry analysis of its specific products through multiple PCR and multiple single-base extension reactions , to determine the genotype of each locus. Background technique [0002] Cardiovascular disease is a serious threat to the health of Chinese people, with a large patient base and a high mortality rate. According to the "China Cardiovascular Disease Report 2017" data, there are about 290 million patients with cardiovascular diseases in my country, including 13 million strokes, 11 million coronary heart diseases, 4.5 million heart failures,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
Inventor 郭惠民张郁陈琦张盼胡志刚
Owner ZHEJIANG DIGENA DIAGNOSTIC TECH CO LTD
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