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Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo

A technology for repelling Vernonia and sesquiterpenoids, applied in the field of medicine, can solve the problems that the use of sesquiterpenoids has not yet been reported and the like

Active Publication Date: 2019-06-28
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After years of research, the drugs for the treatment of vitiligo are mainly concentrated on compounds such as coumarin and flavonoids, and the use of sesquiterpenoids as drugs for the treatment of vitiligo has not been reported yet.

Method used

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  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo
  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo
  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo

Examples

Experimental program
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Effect test

Embodiment

[0035] The sesquiterpene compound isolated from anthelmintic vernonia described in the present invention is tested for measuring the melanin content of mouse B16 melanoma cells:

[0036] Measure the melanin content of B16 melanoma cells by the three sesquiterpene compounds separated:

[0037] Screening model: mouse B16 melanoma cells;

[0038] Cell source: provided by the Cell Bank of the Chinese Academy of Sciences;

[0039] Culture conditions: After culturing cells with 10% fetal bovine serum and 1% double-antibody high-sugar Dulbecco's modified Eagle's medium (DMEM medium) for 24 hours, add different concentrations of drugs and positive controls, respectively, at 48 hours and 48 hours. 72h to measure tyrosine activity and melanin content;

[0040] test methods:

[0041] Protein quantification was determined by Bradford method:

[0042] Completely dissolve the protein standard (5mg / ml BSA), take 10μl and dilute to 100μl, so that the final concentration is 0.5mg / ml, the p...

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Abstract

The invention relates to an application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo. The sesquiterpenoids are obtained by separating and purifying Vernonia anthelmintica seeds and have three sesquiterpenoids, namely Vernodalin, Vernodanol D and vernodolinol, which can promote melanin synthesis. The effects of the three sesquiterpenoids on the melanin content of mouse B16 melanoma cells were investigated. The results showed that compound 1-3 could significantly increase the synthesis of the melanin under the effect of drug concentration without affecting the survival rate of the cells, and could be used as a lead compound for treating the vitiligo.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a sesquiterpene compound vernonia macropicrin, vernitol D and vernodalinol, which can promote the synthesis of melanin, obtained by separating and purifying from the seeds of Vernonia expelling insects. Uses in medicine. Background technique [0002] Vitiligo is a common spontaneous or idiopathic acquired depigmentation disease, which is mainly characterized by depigmentation caused by selective destruction of skin and hair melanocytes. Vitiligo has great differences among different regions and races, people with darker skin are more susceptible to the disease, and the prevalence of Vitiligo in my country is 0.09%-2.7%. One city in each of the six provinces and autonomous regions in my country was selected as the survey point to conduct a multi-regional, large-sample epidemiological study of vitiligo. The results showed that the total prevalence of vitiligo was 0.70%, and the sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/366A61P17/00C07D311/76C07D493/04
CPCY02A50/30
Inventor 阿吉艾克拜尔·艾萨祖力皮牙·买买提阿不拉江·图拉克信学雷
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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