Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo

A technology for repelling Vernonia and sesquiterpenoids, applied in the field of medicine, can solve the problems that the use of sesquiterpenoids has not yet been reported and the like

Active Publication Date: 2019-06-28
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After years of research, the drugs for the treatment of vitiligo are mainly concentrated on compounds such as coumarin and flavonoids, and the use of sesquiterpenoids as drugs for the treatment of vitiligo has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo
  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo
  • Application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] The sesquiterpene compound isolated from anthelmintic vernonia described in the present invention is tested for measuring the melanin content of mouse B16 melanoma cells:

[0036] Measure the melanin content of B16 melanoma cells by the three sesquiterpene compounds separated:

[0037] Screening model: mouse B16 melanoma cells;

[0038] Cell source: provided by the Cell Bank of the Chinese Academy of Sciences;

[0039] Culture conditions: After culturing cells with 10% fetal bovine serum and 1% double-antibody high-sugar Dulbecco's modified Eagle's medium (DMEM medium) for 24 hours, add different concentrations of drugs and positive controls, respectively, at 48 hours and 48 hours. 72h to measure tyrosine activity and melanin content;

[0040] test methods:

[0041] Protein quantification was determined by Bradford method:

[0042] Completely dissolve the protein standard (5mg / ml BSA), take 10μl and dilute to 100μl, so that the final concentration is 0.5mg / ml, the p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an application of sesquiterpenoids separated from Vernonia anthelmintica in preparation of medicaments for treating vitiligo. The sesquiterpenoids are obtained by separating and purifying Vernonia anthelmintica seeds and have three sesquiterpenoids, namely Vernodalin, Vernodanol D and vernodolinol, which can promote melanin synthesis. The effects of the three sesquiterpenoids on the melanin content of mouse B16 melanoma cells were investigated. The results showed that compound 1-3 could significantly increase the synthesis of the melanin under the effect of drug concentration without affecting the survival rate of the cells, and could be used as a lead compound for treating the vitiligo.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a sesquiterpene compound vernonia macropicrin, vernitol D and vernodalinol, which can promote the synthesis of melanin, obtained by separating and purifying from the seeds of Vernonia expelling insects. Uses in medicine. Background technique [0002] Vitiligo is a common spontaneous or idiopathic acquired depigmentation disease, which is mainly characterized by depigmentation caused by selective destruction of skin and hair melanocytes. Vitiligo has great differences among different regions and races, people with darker skin are more susceptible to the disease, and the prevalence of Vitiligo in my country is 0.09%-2.7%. One city in each of the six provinces and autonomous regions in my country was selected as the survey point to conduct a multi-regional, large-sample epidemiological study of vitiligo. The results showed that the total prevalence of vitiligo was 0.70%, and the sta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/366A61P17/00C07D311/76C07D493/04
CPCY02A50/30
Inventor 阿吉艾克拜尔·艾萨祖力皮牙·买买提阿不拉江·图拉克信学雷
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap