Method for detecting paraquat in raw material of Chinese medicinal material

Abstract

The invention discloses a method for detecting paraquat in the raw material of a Chinese medicinal material. Pretreatment comprises the step that a sample is mixed with a first specified amount of extract to form a first mixed liquid; the first mixed liquid is centrifuged, and a centrifuged supernatant liquid A is collected; a bottom layer turbid liquid after supernatant liquid collecting is mixedwith a second specified amount of extract to form a second mixed liquid; the second mixed liquid is centrifuged, and a centrifuged supernatant liquid B is collected; the supernatant liquid A and thesupernatant liquid B are mixed to form a third mixed liquid; the third mixed liquid is mixed with a third specified amount of extract to form a fourth mixed liquid; the fourth mixed liquid is filtered, and the pH of the fourth mixed liquid is adjusted to a specified pH value; the fourth mixed liquid adjusted to the specified pH value is transferred to a solid phase extraction column, and a fifthspecified amount of eluent is added for elution; and a filtrate is collected and removed for measuring. The method fills a gap in the field of traditional Chinese medicine detection, and the detectionlimit can reach 50 mu g / kg.

Description

technical field [0001] The invention relates to the field of detection, in particular to a detection method for paraquat in raw materials of Chinese medicinal materials. Background technique [0002] Paraquat is easily soluble in water and is a high-efficiency herbicide with contact and certain systemic effects, and can be quickly absorbed by the green tissues of plants, causing them to die. [0003] Simultaneously paraquat is extremely toxic to people, and there is no specific antidote, and the mortality rate of oral poisoning is extremely high, which can reach more than 90% mortality rate. Paraquat can be absorbed through the intact skin, respiratory tract and digestive tract, and then distributed to various tissues and organs throughout the body with the blood. Systemic poisoning in the human body can cause damage to multiple systems, especially lung damage, and liver, kidney, and nervous system damage . [0004] At present, there is no relevant standard method for the ...

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Problems solved by technology

[0004] At present, there is no relevant standard method for the detection of paraquat in the domestic drug testing, especially for traditional Chinese medicine, because the pre-processing of the medicinal materials is generally relatively simple and easy to Pesticides sprayed during the planting process remain, so the raw materials of traditional Chinese medicine are more likely to be polluted by highly toxic pesticides

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