A kind of hyperbranched polychitosan and its preparation method and application

A technology of hyperbranched polychitosan, which is applied in the preparation of new drug carriers, drug and gene carriers of hyperbranched polychitosan, to achieve the effect of improving intracellular gene release ability, solubility, and controlled release ability

Active Publication Date: 2021-07-02
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of existing gene therapy and gene editing vectors, to provide a kind of hyperbranched polychitosan and its preparation method and application

Method used

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  • A kind of hyperbranched polychitosan and its preparation method and application
  • A kind of hyperbranched polychitosan and its preparation method and application
  • A kind of hyperbranched polychitosan and its preparation method and application

Examples

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Effect test

Embodiment 1

[0024] Dissolve 0.7g of bis(N-hydroxysuccinimide) suberate in 10mL of DMSO (liquid A), dissolve 1g of PEI (MW=25000Da, PEI-25) in 20mL of ultrapure water (liquid B) , slowly add liquid B to liquid A under stirring, complete the dropwise addition in 30 minutes, and react for 3 hours; take another 1g of chitin (MW=1000Da) and dissolve it in 20mL ultrapure water (liquid C), and then add liquid C Slowly add it dropwise to the reaction system for 30 minutes, then stir and react at room temperature for 3 hours, transfer the reaction solution to a dialysis bag (MWCO=1000Da), use deionized water to dialyze to remove impurities, and finally freeze-dry to obtain hyperbranched polychitosan HBPC .

Embodiment 2

[0026] Dissolve 0.7g of bis(N-hydroxysuccinimide) suberate in 10mL of DMSO (liquid A), dissolve 1g of PEI (MW=25000Da, PEI-25) in 20mL of ultrapure water (liquid B) , slowly add liquid B to liquid A under stirring, complete the dropwise addition in 30 minutes, and react for 3 hours; take another 1g of chitin (MW=500) and dissolve it in 20mL ultrapure water (liquid C), and then add liquid C Slowly add it dropwise to the reaction system for 30 minutes, then stir and react at room temperature for 3 hours, transfer the reaction solution to a dialysis bag (MWCO=500Da), use deionized water to dialyze to remove impurities, and finally freeze-dry to obtain hyperbranched polychitosan HBPC .

Embodiment 3

[0028] Dissolve 0.7g of bis(N-hydroxysuccinimide) suberate in 10mL of DMSO (liquid A), and dissolve 0.25g of hyperbranched polyamidoamine (MW=6909Da) in 20mL of ultrapure water (liquid B ), slowly drop B liquid into A liquid under stirring state, complete the dropwise addition in 30min, and react for 3h; take another 1g chitin (MW=1000Da) and dissolve it in 20mL ultrapure water (C liquid), and then C solution was slowly added dropwise to the reaction system, added dropwise for 30min, then stirred at room temperature for 3h, the reaction solution was transferred to a dialysis bag (MWCO=1000Da), and impurities were removed by dialysis in deionized water, and finally freeze-dried to obtain hyperbranched polychitosan HBPC.

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Abstract

The invention provides a hyperbranched polychitosan and its preparation method and application. The invention adopts bifunctional reagent to graft chitosan on hyperbranched macromolecular skeleton, and prepares hyperbranched polychitosan with hyperbranched macromolecular skeleton as inner core and chitin as terminal group. Hyperbranched polychitosan with different structures can be synthesized by changing the type and size of the polymer backbone, as well as the feed ratio of the polymer backbone, bifunctional coupling reagent and chitin. The hyperbranched polychitosan provided by the present invention has a special hyperbranched structure, which overcomes the structural limitations of the original linear chitosan, and at the same time, because the hyperbranched polychitosan has a chitin end group with a pKa of about 6.5, it has a pH Environmentally responsive characterization of sensitive charge reversals. The hyperbranched chitosan exhibits excellent biocompatibility and efficient gene delivery performance, and has broad application prospects in the fields of gene therapy and gene editing.

Description

technical field [0001] The present invention relates to a drug and gene carrier of hyperbranched polychitin, in particular to a pH-responsive hyperbranched chitin conjugate and its application in the field of gene drug delivery, which belongs to the field of new drug carrier in the field of drug delivery Preparation methods and techniques. Background technique [0002] Gene therapy introduces foreign genes into target cells to repair defective genes that cause diseases or inhibit harmful genes that cause diseases, so as to restore the body to normal functions and achieve the purpose of treating diseases [1. Chinese invention patent, authorization number ZL201010601008. 5. The name of the invention is: glutathione-modified chitosan copolymer used as a non-viral gene carrier material and its preparation and application]. Gene therapy technology provides new treatment methods for diseases that cannot be cured by conventional medical means. Gene editing refers to accurately id...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00C08B37/08A61K47/36A61K48/00
CPCA61K47/36A61K48/0041C08B37/003C08G81/00
Inventor 张树彪陈会英蓝浩铭范雪枫马羽
Owner DALIAN NATIONALITIES UNIVERSITY
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