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Redox response hyperbranched chitosan and preparation method and application thereof

A technology of hyperbranched poly-chitin, which is applied in pharmaceutical formulations, medical preparations of non-active ingredients, gene therapy, etc., to achieve the effects of improving gene release ability and solubility, enhancing controlled release ability, and improving cell uptake ability

Active Publication Date: 2019-06-28
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of existing gene therapy and gene editing vectors, to provide a redox responsive hyperbranched polychitosan and its preparation method and application

Method used

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  • Redox response hyperbranched chitosan and preparation method and application thereof
  • Redox response hyperbranched chitosan and preparation method and application thereof
  • Redox response hyperbranched chitosan and preparation method and application thereof

Examples

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Embodiment 1

[0025]Dissolve 0.012g of 3,3'-dithiodipropionate bis(N-hydroxysuccinimide) in 10mL of dimethyl sulfoxide, slowly add 0.5g of polyethyleneimine (MW=1800Da) dropwise React in the above solution at room temperature for 3 hours, transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 1000 Da, perform dialysis with deionized water, and then freeze-dry to obtain a solid redox-responsive hyperbranched polymer. Dissolve 0.4g of bis(N-hydroxysuccinimide) suberate in 10mL of dimethyl sulfoxide, and dissolve another 0.5g of chitin (MW=1000Da) in 20mL of ultrapure water, and slowly Add dropwise (dropping for about 30 minutes), take 0.05 g of the prepared redox-responsive hyperbranched polymer skeleton and dissolve it in 20 mL of ultrapure water, and then slowly add this solution dropwise to the above solution (dropping for about 30 minutes). Stirring and reacting for 4 hours, the reaction solution was transferred to a dialysis bag with a molecular weight cut-of...

Embodiment 2

[0027] Dissolve 0.012g of 3,3'-dithiodipropionate bis(N-hydroxysuccinimide) in 10mL of dimethyl sulfoxide, slowly add 0.5g of polyethyleneimine (MW=600Da) dropwise React in the above solution at room temperature for 3 hours, transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 1000 Da, perform dialysis with deionized water, and then freeze-dry to obtain a solid redox-responsive hyperbranched polymer. Dissolve 0.4g of bis(N-hydroxysuccinimide) suberate in 10mL of dimethyl sulfoxide, and dissolve another 0.5g of chitin (MW=1000Da) in 20mL of ultrapure water, and slowly Add dropwise (dropping for about 30 minutes), take 0.05 g of the prepared redox-responsive hyperbranched polymer skeleton and dissolve it in 20 mL of ultrapure water, and then slowly add this solution dropwise to the above solution (dropping for about 30 minutes). Stirring and reacting for 4 hours, the reaction solution was transferred to a dialysis bag with a molecular weight cut-of...

Embodiment 3

[0029] Dissolve 0.012g of 3,3'-dithiodipropionate bis(N-hydroxysuccinimide) in 10mL of dimethyl sulfoxide, slowly drop 0.5g of hyperbranched polyamidoamine (MW=1430Da) Add it to the above solution and react at room temperature for 3 hours, transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 1000 Da, perform dialysis with deionized water, and then freeze-dry to obtain a solid redox-responsive hyperbranched polymer. Dissolve 0.4g of bis(N-hydroxysuccinimide) suberate in 10mL of dimethyl sulfoxide, and dissolve another 0.5g of chitin (MW=1000Da) in 20mL of ultrapure water, and slowly Add dropwise (dropping for about 30 minutes), take 0.05 g of the prepared redox-responsive hyperbranched polymer skeleton and dissolve it in 20 mL of ultrapure water, and then slowly add this solution dropwise to the above solution (dropping for about 30 minutes). Stirring and reacting for 4 hours, the reaction solution was transferred to a dialysis bag with a molecula...

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Abstract

The invention provides redox response hyperbranched chitosan and a preparation method and application thereof. A bifunctional reagent containing a disulfide bond is adopted for crosslinking a low molecular weight hyperbranched polymer, then, chitin is grafted to a skeleton of the crosslinked hyperbranched polymer, and the redox response hyperbranched chitosan adopting the hyperbranched polymer skeleton containing the disulfide bond as the core and adopting the chitin as end groups is prepared. The redox response hyperbranched chitosan has the special hyperbranched structure, the structure limit of existing linear chitosan is overcome, meanwhile, due to the fact that the periphery of the hyperbranched chitosan is chitosan with pKa being about 6.5 and has the environment response characteristics of pH sensitive charge reversal and redox degradation. The environment response hyperbranched chitosan has the good biological compatibility and the efficient gene deliver performance, and the wide application prospect is achieved in the fields of gene therapy and gene edition.

Description

technical field [0001] The invention relates to a redox responsive hyperbranched polychitin drug and gene carrier, in particular to a redox responsive hyperbranched chitin conjugate with pH responsiveness and its application in the field of gene drug delivery The invention belongs to the preparation method and technology of a novel drug carrier in the field of drug delivery. Background technique [0002] Gene therapy introduces foreign genes into target cells to repair defective genes that cause diseases or inhibit harmful genes that cause diseases, so as to restore the body to normal functions and achieve the purpose of treating diseases [1. Chinese invention patent, authorization number ZL201010601008. 5. The name of the invention is: glutathione-modified chitosan copolymer used as a non-viral gene carrier material and its preparation and application]. Gene therapy technology provides new treatment methods for diseases that cannot be cured by conventional medical means. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00A61K47/36A61K48/00
Inventor 张树彪陈会英马羽蓝浩铭范雪枫
Owner DALIAN NATIONALITIES UNIVERSITY
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