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Single nucleotide polymorphism (SNP) molecular marker related to character of prolificacy during single birth of sheep, and detection primer group, detection kit and application of SNP molecular marker

A technology of molecular markers and primer sets, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve problems that restrict the development of the sheep industry, and achieve true and reliable detection results, convenient operation, and high sensitivity Effect

Active Publication Date: 2019-07-19
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

But its single lamb and seasonal estrus greatly restrict the development of sheep industry

Method used

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  • Single nucleotide polymorphism (SNP) molecular marker related to character of prolificacy during single birth of sheep, and detection primer group, detection kit and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker related to character of prolificacy during single birth of sheep, and detection primer group, detection kit and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker related to character of prolificacy during single birth of sheep, and detection primer group, detection kit and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A way to use Sequenom A method of detecting sheep BMPR1B genotype and predicting the average litter size of multiparous ewes by SNP technology

[0052] 1. Experimental materials

[0053] 380 small-tailed Han sheep were selected as the test objects.

[0054] 2. Reagents and instruments

[0055] Reagent: Complete Genotyping Reagent Kit for Compact 384;

[0056] Gene Amplification: ABI 9700 384 Dual;

[0057] Mass spectrometry spotting: MassARRAY NanodispenserRS1000;

[0058] Mass spectrometry: MassARRAY Compact System;

[0059] All reagents and instruments were purchased from Beijing Compass Biotechnology Co, Ltd.

[0060] 3. Genomic DNA extraction

[0061] Take 1ml of sheep's jugular vein blood and anticoagulate it with EDTA. First, the red blood cell lysate is lysed to remove DNA-free red blood cells. The nucleus lysate lyses the cells to release genomic DNA. Then the protein precipitation solution selectively precipitates to remove the protein. Finally, th...

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Abstract

The invention provides a single nucleotide polymorphism (SNP) molecular marker related to a character of prolificacy during single birth of sheep, and a detection primer group, detection kit and application of the SNP molecular marker, and belongs to the technical field of sheep SNP molecular markers. Based on a sheep genome sequence information version number Oar_v4.0, the SNP molecular marker isobtained by A / G base mutation at the 29380965 bp site on the sixth chromosome of the sheep, and has significant correlation with the prolificacy character of the sheep. The lambing performance of thesheep to be tested can be determined by typing the SNP site. The method for detecting the genotype at the SNP site of the sheep is high in sensitivity, high in accuracy and higher in cost performance, and the SNP sites can be automatically detected. In a sheep breeding process, AA-type individuals can be selected, so that fecundity of the sheep is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular markers and genetic breeding, and specifically relates to a SNP molecular marker related to the trait of single litter and multiple lambs in sheep, a detection primer set, a detection kit and applications thereof. Background technique [0002] Lamb size is a complex quantitative trait regulated by factors such as genetics, environment and hormone levels. Sheep is one of the excellent livestock species in our country. But its lambing and seasonal estrus greatly restrict the development of sheep industry. Therefore, increasing the number of lambs per litter is an important measure to improve the economic benefits of production. The application of genomics research can screen out candidate genes and molecular markers related to sheep reproductive traits at the level of the entire genome, enabling scholars to better understand the genetic mechanism of sheep's multiple lamb traits, which can contri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2533/101C12Q2565/627C12Q2521/301
Inventor 储明星文禹梁贺小云
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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