A screening method for immunoactive peptides

An immunoactive peptide and screening method technology, applied in the field of immunoactive peptide screening, can solve the problems of cumbersome experimental process, reduced screening efficiency of active peptides, excessive separation of components, etc. The effect of the process is fast

Active Publication Date: 2022-07-26
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have too many separated components, the experimental process is cumbersome, and the screening efficiency of active peptides is greatly reduced.

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  • A screening method for immunoactive peptides
  • A screening method for immunoactive peptides
  • A screening method for immunoactive peptides

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Embodiment Construction

[0039] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific implementation disclosed below.

[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention.

[0041] The term "MHC" as used herein is short for major histocompatibility complex, a collective term for a group of genes encoding mamma...

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Abstract

The present invention relates to a screening method for immunologically active peptides. The screening method comprises the following steps: S1, hydrolyzing at least one protein containing immunologically active peptides by a hydrolase to obtain a peptide library composed of immunologically active peptides, wherein the peptide library is The peptide sequence of the peptide was obtained by mass spectrometry; S2, to screen out the peptide sequence with strong binding to MHC class II molecules; S3, to synthesize the peptide sequence from S2; S4, to construct an LPS-induced cell model to verify the synthesis of S3 The immune activity of the polypeptides. The invention can avoid the cumbersome identification of the peptide sequence and the binding site, and directly obtain the polypeptide bound to the MHC class II molecule; and can simultaneously explain the reactogenicity and immunogenicity of the polypeptide combined with the MHC class II molecule, so that the data is more accurate. Perfect, the process of obtaining peptides is faster and more efficient.

Description

technical field [0001] The present invention relates to the field of biotechnology, and more particularly, to a screening method for immunoactive peptides. Background technique [0002] In recent years, the phenomenon of various diseases caused by the decline of the body's immunity caused by aging, obesity, decreased sleep time or quality, smoking and drinking and other bad habits has become more and more serious. It has become an urgent need for people to seek suitable immunomodulators to improve human immunity. At present, the method to obtain immunologically active peptides is mainly separation and purification technology, and the means to obtain immunologically active peptides by using online prediction methods combined with experimental screening are very limited. The current separation and purification technology mainly separates the peptides in the peptide library into different components based on various molecular characteristics such as molecular weight, ionic str...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C12Q1/6869C12P21/06
CPCG01N33/68C12Q1/68C12P21/06
Inventor 程云辉黄璐许宙陈茂龙文李银波盛灿梅毛田米
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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