A strain of Bacillus proteolyticus and its application in controlling root-knot nematode

A bacillus and protein-dissolving technology, applied in the application, nematicide, bacteria and other directions, can solve the problems of high toxicity of chemical nematicides, easy to produce drug resistance, pollute the environment, etc., and achieve good control effect, efficient control effect and high efficiency. stable effect

Active Publication Date: 2020-12-25
安徽沛然生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, chemical nematicides are mainly used to control root-knot nematodes in production, but chemical nematicides generally have many problems such as high toxicity, environmental pollution, and easy to produce drug resistance, so in practice people gradually reduce the use of chemical agents

Method used

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  • A strain of Bacillus proteolyticus and its application in controlling root-knot nematode
  • A strain of Bacillus proteolyticus and its application in controlling root-knot nematode

Examples

Experimental program
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Embodiment 1

[0014] Embodiment 1: Isolation, purification and identification of Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 strain

[0015] 1. Isolation and purification of Bacillus proteolyticus Bp-196 strain

[0016] The Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 bacterial strain of the present disclosure is obtained by adopting the soil dilution method to separate from the cucumber rhizosphere soil where root-knot nematode disease occurs in cucumber fields planted in Langfang City, Hebei Province, and the separation method is as follows:

[0017] Soil samples were evenly collected from the cucumber rhizosphere in the cucumber planting field in Langfang City, Hebei Province, a total of six samples, each with 10 g, and the collected 6 soil samples were uniformly mixed. Add 60g of the mixed soil sample into 1000mL distilled water and stir evenly. After standing for 1min, take the supernatant, and place the supernatant in a water bath at 80°C for 30min. During the wat...

Embodiment 2

[0027] Embodiment 2: the nematicide effect of Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 bacterial strain

[0028] 1. Fermentation broth preparation of Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 strain

[0029] The Bacillus proteolyticus Bp-196 strain was inoculated into the fermentation medium, and cultured at 30° C. at a rotational speed of 220 rpm for 3 days to obtain a fermentation liquid of the Bacillus proteolyticus Bp-196 strain. Wherein, the fermentation medium includes: 5g / L yeast powder, 10g / L peptone, 10g / L sodium chloride, and the volume is adjusted to 1L with distilled water.

[0030] 2. Preparation of Nematodes for Experiments

[0031] Root-knot nematodes were collected from the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. Take out the root system of the pepper with root-knot nematode, rinse it gently with water, carefully remove the egg mass from the surface of the root syste...

Embodiment 3

[0040] Embodiment 3: the nematicide effect of Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 bacterial strain

[0041] 1. Fermentation broth preparation of Bacillus proteolyticus (Bacillus proteolyticus) Bp-196 strain

[0042] The Bacillus proteolyticus Bp-196 strain was inoculated into the fermentation medium, and cultured at 30° C. at a rotational speed of 220 rpm for 3 days to obtain a fermentation liquid of the Bacillus proteolyticus Bp-196 strain. Wherein, the fermentation medium includes: 5g / L yeast powder, 10g / L peptone, 10g / L sodium chloride, and the volume is adjusted to 1L with distilled water.

[0043] 2. Preparation of Nematodes for Experiments

[0044] Root-knot nematodes were collected from the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. Take out the root system of the pepper with root-knot nematode, rinse it gently with water, carefully remove the egg mass from the surface of the root syste...

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Abstract

The disclosure relates to a strain of proteolytic bacillus and its application in preventing and controlling root-knot nematodes. The strain is Bacillus proteolyticus Bp-196, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on September 21, 2018, and the strain preservation number is CGMCC NO.16525. The strain is a bacterium isolated from the rhizosphere soil of cucumbers where root-knot nematode disease occurs. It has a good control effect on root-knot nematodes, is conducive to the early prevention and control of root-knot nematodes, and helps to achieve safe and pollution-free vegetables. Production.

Description

technical field [0001] The disclosure relates to the field of microbial application, in particular to a strain of Bacillus proteolyticus and its application in preventing and controlling root-knot nematodes. Background technique [0002] Plant parasitic nematodes exist in many countries and regions in the world, and there are various types. Among them, root-knot nematode is one of the most important plant parasitic nematodes. With the rapid increase of planting area of ​​protected vegetables in my country and the continuous improvement of vegetable multi-cropping index, the occurrence area of ​​root-knot nematode is gradually expanding, which can reduce vegetable production by 20% to 30%. %, or even 60% to 70%, seriously restricting the development of the facility vegetable industry. Root-knot nematode (M. incognita) hosts more than 3,000 kinds of plants, and can infect the roots of almost all cultivated plants. [0003] At present, chemical nematicides are mainly used to c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P5/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 茆振川谢丙炎杨宇红殷楠凌键李彦
Owner 安徽沛然生物科技有限公司
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