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Pig Seneca virus ELISA antibody detection method and application thereof

An antibody detection, pig Seneca technology, applied in the field of immunological detection, can solve the problems of no commercial detection kits, unsatisfactory specificity and sensitivity, etc.

Inactive Publication Date: 2019-09-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, foreign scholars have established SVV VP1ELISA antibody detection method to determine whether pigs are infected by SVV, but its specificity and sensitivity are not ideal, and there is no commercial detection kit in my country

Method used

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  • Pig Seneca virus ELISA antibody detection method and application thereof
  • Pig Seneca virus ELISA antibody detection method and application thereof
  • Pig Seneca virus ELISA antibody detection method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0029] 1 Materials and methods

[0030] 1.1 Viruses, serum, plasmids and reagents

[0031] SVV SD strain, neutralizing antibody-positive pig serum, neutralizing antibody-negative serum and challenge serum are preserved by our laboratory. 3249 clinical swine serum samples were collected from 126 pig farms in Shandong and Jiangsu provinces. pET-28a(+), pET-32a(m)E.coli BL21(DE3) are preserved by our laboratory. The SVV SD strain involved in the experiment process is a conventional strain known to those skilled in the art, and the carrier is a conventional carrier that can be purchased from the market.

[0032] HRP-labeled Staphylococcus aureus protein A (HRP-SPA) antibody was purchased from Boster Biotechnology Co., Ltd., TMB chromogenic solution was purchased from Shanghai Biyuntian Biotechnology Co., Ltd., restriction enzymes and T4 ligase were purchased from TaKaRa Biotechnology Co., Ltd., the ultra-sensitive ECL chemiluminescence kit was the product of Pierce Company, the...

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Abstract

The invention provides a pig Seneca virus ELISA antibody detection method and an application thereof. The pig Seneca virus ELISA antibody detection method uses SVV 3AB recombinant protein as coating antigen. The 3AB ELISA antibody detection method provided by the invention can be used as a detection tool for clinical serum detection, and lays a foundation for the diagnosis of diseases and the research and development of an SVV antibody detection kit.

Description

technical field [0001] The invention belongs to the field of immunological detection methods, and in particular relates to a detection method and application of porcine Seneca Valley virus ELISA antibody. Background technique [0002] Seneca Valley virus (Senecavirus, SVV) belongs to Picornaviridae, Seneca Valley virus genus, very similar to members of the Cardiovirus genus, is a non-enveloped, single-stranded positive-sense RNA virus. In recent years, SVV infections have been reported in the United States, Brazil, Thailand, Australia, Italy, Canada, and China, indicating that SVV has evolved and spread rapidly. Infected herds cause vesicular disease and epidemic transient neonatal loss (ETNL). The clinical symptoms of the disease are similar to vesicular animal diseases such as foot-and-mouth disease (FMD), swine vesicular disease (SVD), vesicular stomatitis (VS) and vesicular eruption (VE), which endanger the healthy development of the pig industry. [0003] SVV has geno...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/68G01N33/535
CPCG01N33/535G01N33/56983G01N33/6893
Inventor 姜平延君芳范慧白娟李亮
Owner NANJING AGRICULTURAL UNIVERSITY