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Device for directionally fixing irregularly-shaped tentacles of living insect and manufacturing method of device

A technology of directional fixation and manufacturing method, which is applied in the directions of measuring devices, preparation and sampling of samples for testing, can solve the problems of thick antennae cuticle, low light transmittance, and difficulty in precise positioning operations, and can meet the requirements of light transmittance, High experimental efficiency, easy to observe the effect

Pending Publication Date: 2019-09-27
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a device for orienting and fixing antennae with irregular shapes of living insects and its manufacturing method. According to the site requirements, the directional fixation of the specific angle of the irregular antennae can be realized, which can meet the single-sensor experiment micromanipulation of higher difficulty level, and solve the problem of specific angle fixation, thick horny layer of the antennae, low light transmittance, and puncture of the recording electrode. It is easy to cause the target to move and the distance between the single sensors is only 2-3 microns, making it difficult to accurately locate the operation

Method used

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  • Device for directionally fixing irregularly-shaped tentacles of living insect and manufacturing method of device
  • Device for directionally fixing irregularly-shaped tentacles of living insect and manufacturing method of device
  • Device for directionally fixing irregularly-shaped tentacles of living insect and manufacturing method of device

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Effect test

Embodiment 1

[0043] Such as figure 1 and figure 2 As shown, a device for directional fixing of antennae with irregular shapes of live insects comprises a glass slide 1, an antenna pad 6, an insect body pad 3, a small water storage well device 2, and an ultraviolet glue 5, and the small water storage well device 2 is fixedly arranged on In the middle of the slide glass 1, the tentacles pad 6 is arranged on the slide glass 1 and the tentacles 6 are located in the small water storage well device 2, and the worm body pad 3 is arranged on the slide glass 1 and the worm body pad 3 is located on the slide 1. In the small water storage well device 2, the insect body pad 3 is located at the side end of the antennae pad 6 and is in contact with the side of the antennae pad 6, the upper surface of the insect body pad 3 is provided with an adhesive layer 7, and the antennae pad 6 and the insect body pad The connection of 3 and the tentacles 6 are coated with ultraviolet glue 5;

[0044] The structu...

Embodiment 2

[0062] Orientation and fixation of antennae of live insects with irregular shape in embodiment 1 to the directional fixation of live antennae in electrophysiological experiment of single sensilla of Tephritidae Guava fruit fly

[0063] The adult fruit flies of the family Tephritidae are divided into three segments: head, thorax and abdomen, with a total length of about 5-8mm; the antennae of the fruit fly are awn-shaped, divided into stalk, pedicel and whip; the total length of the antennae is 900-1000μm, Flagellation width ~ 100 μm; irregular geometric shape and very dense sensilla, integrating sensory organs with multiple functions such as smell, hearing, temperature and humidity, and gravity; in order to comprehensively study the working mode of antennae, understand the dorsal, ventral The electrophysiological responses of different types of single sensory organs in the lateral, medial and lateral antennae to external stimuli need to be oriented and fixed. The specific steps...

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Abstract

The invention discloses a device for directionally fixing the irregularly-shaped tentacles of a living insect and a manufacturing method of the device and belongs to the technical field of experimental sample processing. The device comprises a glass slide, a tentacle cushion table, an insect body cushion table, a water storage small well device and an ultraviolet adhesive; the water storage small well device is fixedly arranged at the middle of the glass slide; the tentacle cushion table is arranged on the glass slide, and is located in the water storage small well device; the insect body cushion table is arranged on the glass slide, and is located in the water storage small well device; the insect body cushion table is located to one side end of the tentacle cushion table and contacts with one side surface of the tentacle cushion table; an adhesive layer is arranged on the upper surface of the insect body cushion table; and the ultraviolet adhesive coats the joint of the tentacle cushion table and the insect body cushion table and the tentacle cushion table. According to the device for directionally fixing the irregularly-shaped tentacles of the living insect and the manufacturing method of the device of the invention, the directional fixation of tentacles in irregular shapes is realized by means of the integration of a plurality of devices, and single-sense observation, positioning and recording are facilitated, experiment efficiency is high, cost is low, and the device can be repeatedly used.

Description

technical field [0001] The invention relates to a device for orienting and fixing antennae of living insects with irregular shapes and a manufacturing method thereof, belonging to the technical field of experimental sample processing. Background technique [0002] The antennae are important sensory organs of insects. Various types of micron-scale sensilla are densely distributed on the surface of insect antennae. Single sensilla is the basic structural unit of insect antennae receptors. It is an information receiving device for insects to sense the external environment and communicate. It can sense physical, mechanical or chemical stimulation. In the process of seeking mates, finding hosts or prey habitats, insects mainly complete chemical and physical stimuli through antennal sensory organs sensing pheromones, host plant volatiles, sound and air. It plays an important role in behaviors such as laying eggs, mating, and avoiding predators. For example, the cabbage butterfly...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/2806Y02A50/30
Inventor 曹军刘一淼郭凯飞代曼刘晓飞叶辉
Owner YUNNAN UNIV
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