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Bacillus amyloliquefaciens strain, bactericide, preparation method thereof and application thereof for preventing fresh ginger bacterial wilt

A technology for dissolving amyloid spores and bacterial wilt, applied in the field of microorganisms, can solve the problems of unseen research and application reports on ginger bacterial wilt, achieve good inhibition effect, avoid chemical residues, and broad development prospects

Pending Publication Date: 2019-10-25
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no relevant research and application reports on the use of Bacillus amyloliquefaciens to control ginger bacterial wilt

Method used

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  • Bacillus amyloliquefaciens strain, bactericide, preparation method thereof and application thereof for preventing fresh ginger bacterial wilt
  • Bacillus amyloliquefaciens strain, bactericide, preparation method thereof and application thereof for preventing fresh ginger bacterial wilt
  • Bacillus amyloliquefaciens strain, bactericide, preparation method thereof and application thereof for preventing fresh ginger bacterial wilt

Examples

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Effect test

Embodiment 1

[0027] A strain of Bacillus amyloliquefaciens provided by the present invention, the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain is named as Bacillus amyloliquefaciens TR-01 (Bacillus amyloliquefaciens TR-01), which is preserved in the General Microorganism Center of China Microorganism Culture Preservation Management Committee , the preservation number is: CGMCCNo.13485, the preservation time is December 23, 2016, and the preservation address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, postal code 100101. The isolation and screening process of the Bacillus amyloliquefaciens TR-01 is as follows:

[0028] 1. Isolation and purification of bacteria for screening

[0029] Take out the healthy ginger plant and the rhizosphere soil in the fields that suffer from ginger bacterial wilt from Jinhua, Hangzhou and other places, put 10g of soil into a sterilized 200ml triangular flask, and the...

Embodiment 2

[0044] A preparation of Bacillus amyloliquefaciens TR-01 inoculum of the present invention, the Bacillus amyloliquefaciens TR-01 obtained in Example 1 is made into slanted thallus, then inoculated into liquid LB medium, and then transferred to a fermenter for fermentation , prepare different dosage forms according to different application aspects:

[0045] (1) Liquid preparation: use 500mL Erlenmeyer flask to fill 100ml of LB medium, routinely sterilize, after cooling, wash the slant with sterile water on the ultra-clean workbench and inoculate it into the fermentation Erlenmeyer flask, shake the flask and culture at 30°C , 180r / min, cultured for 24h; immediately transferred to fermenter for fermentation, preparation and sterilization of LB medium in bacterial fermenter, inoculated and fermented after cooling, 30°C, 180r / min, cultured for 48h to stop fermentation, and the fermented liquid reached every One milliliter of bacteria liquid contains 10 9 ~10 12 spores, and then u...

Embodiment 3

[0049] A kind of bacterial agent that Bacillus amyloliquefaciens TR-01 of the present invention makes is to the control effect test of ginger bacterial wilt:

[0050] Select the plots where ginger bacterial wilt falls on all the year round, choose the field natural disease area 0.lmh 2 , divided into 30 plots, each plot is about 30m 2 , arranged in random blocks, and the planting method was uniformly carried out according to the local cultivation system. When the first diseased plant appeared in the ginger field, three concentrations of TR-01 strain suspension were used: 200ml / mu, 300ml / mu, and 400ml / mu. , and 3% Kejunkang wettable powder to irrigate the roots, each treatment was repeated 3 times, with clear water as a control, sampling and investigating the incidence of ginger bacterial wilt at five o'clock in the peak period of onset of ginger bacterial wilt, disease classification: 0 grade: asymptomatic; Grade 1: 1 / 4 leaves are wilted and dead; Grade 2: 1 / 4~1 / 2 leaves are ...

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Abstract

The invention discloses a bacillus amyloliquefaciens strain. The bacillus amyloliquefaciens strain is named as bacillus amyloliquefaciens TR-01, and preserved at the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.13485, and the preservation time is 23th Dec. 2016. The invention further discloses a bactericide manufactured from the strain, a preparationmethod of the bactericide and application of the bactericide for preventing fresh ginger bacterial wilt. The adopted bacillus amyloliquefaciens strain or the bactericide manufactured from the strainhas the good inhibition effect on fresh ginger bacterial wilt germs, can avoid chemical residues, is environmentally friendly, generates no drug resistance, is good in safety and low in cost and has the wide development prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus amyloliquefaciens, a bacterial agent, a preparation method thereof and an application in preventing and treating ginger bacterial wilt. Background technique [0002] Ginger bacterial wilt, commonly known as ginger blast, is a soil-borne disease that commonly occurs in subtropical and tropical regions. This disease just occurred in China in the 1950s, and it was very serious in the main ginger cultivation areas of China. These areas caused 20% to 30% losses all the year round due to the harm of ginger blast, and some serious field areas occurred. The loss caused can be as high as 70%. It is a disease that seriously affects the yield and quality of ginger in China. [0003] Ginger bacterial wilt is a bacterial disease caused by Ralstonia solanacearum attacking ginger, which mainly affects the near-ground and underground parts of ginger. Its...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 朱璞周秦郭子卿林贤锐程林润王轶朱一丹徐金晶
Owner JINHUA ACAD OF AGRI SCI