dsrna, coding gene, preparation method and application thereof based on Periplaneta americana orco gene design

Abstract

The invention discloses dsRNA designed based on an Orco gene of periplaneta americana, and a coding gene, a preparation method and an application thereof. The dsRNA is a double-stranded RNA composed of a nucleotide sequence shown in SEQ ID NO.1 and used as a positive-sense strand and a nucleotide sequence reversely complementary to the nucleotide sequence shown in SEQ ID NO.1 and used as an antisense strand. The dsRNA can be widely applied in preparation of products for preventing and treating the periplaneta americana, interfering the chemical communication of the periplaneta americana or controlling the mating and reproduction of adult periplaneta americana, or preventing and controlling the periplaneta americana and other insects having the same gene target sequence. By blocking key genes of an olfactory system of periplaneta americana, the induction effect of sex pheromone to male insects is inhibited, mating and reproduction of the periplaneta americana can be effectively controlled, and a new technology and strategy are provided for prevention and control of the periplaneta americana.

Description

technical field [0001] The invention relates to the field of sanitary pest control, in particular to a dsRNA designed based on the Periplaneta americana Orco gene, a coding gene, a preparation method and application thereof. Background technique [0002] Cockroaches are a class of important worldwide sanitary pests, causing serious harm to human health and public health. Cockroaches carry a variety of pathogenic microorganisms, which can cause infectious diseases such as cholera, anthrax, and tuberculosis. Cockroach excrement and insect sloughs are also important allergens, which can easily cause allergic reactions such as skin rashes and asthma. Therefore, curbing the reproduction and population expansion of cockroaches from the source has become an important issue that needs to be solved urgently and is becoming urgent in the field of sanitary pest control. [0003] The American cockroach (Periplaneta americana L.) is one of the most common species of cockroaches in the ...

AI Technical Summary

Problems solved by technology

However, so far, the functional exploration of Orco has mainly focused on basic research fields such as gene identification, gene differentiation, olfactory coding and evolution; the research objects are mostly concentrated in complete metamorphosis insects, such as Drosophila, mosquitoes, moths, etc.; behavior analysis is only found in In terms of insect aggregation, repulsion, migration, etc., there is very limited research on the mating behavior, especially the function of the Orco gene in the mating behavior mechanism of cockroaches

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