Method of establishing aseptic culture system for mature seed embryos of oil-use paeonia ostia 'feng dan'

Abstract

The invention relates to a method of establishing an aseptic culture system for mature seed embryos of oil-use paeonia ostia 'feng dan', and belongs to the field of plant tissue culture. The method includes the following steps of (1) selecting seeds; (2) removing outer seed coats; (3) washing and soaking the seeds after coat removal; (4) conducting disinfecting treatment; (5) removing endosperms;(6) conducting inoculation and culture. According to the method, the mature seed embryos of the paeonia ostia 'feng dan' are selected and used, and after strict selection, coat removal, soaking, disinfection, inoculation and culture, four weeks after the start of the constructed high-efficiency aseptic culture system, the germination rate is >=80%, the contamination rate is <=5%, and no browning occurs. The problem of browning in the establishment of the aseptic culture system of a peony tissue culture technology using scale buds or soil buds as explants is thoroughly solved, the pollution rate during the establishment of the system is greatly reduced, and the application efficiency of the peony tissue culture technology in the establishment of the aseptic culture system in production andpractice is improved.

Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for establishing an aseptic culture system for mature seed embryos of peony 'Fengdan' for oil. Background technique

[0002] peony( Paeonia suffruticosa Andr. ) belongs to Paeoniaceae ( Paeoniaeeae ) Paeonia ( Paeonia ) Perennial deciduous subshrubs. 'Feng Dan' ( Paeonia ostii 'Feng Dan' ) is a kind of medical and oil species gradually formed after long-term planting and cultivation of the original species of Yangshan peony. Oil peony 'Fengdan' can be developed and utilized as a new type of woody grain and oil plant resource. Compared with other oil crops, oil peony has the following characteristics: high yield, high oil content, high quality, and low cost. Therefore, vigorously promoting the planting of peony for oil is of great significance to promote the production of grain and oil in my country, change the consumption structure of edible oil an...

Examples

Embodiment 1

[0033]A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:

[0034] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;

[0035] Step 2. Use needle-nose pliers to carefully remove the outer testa of the oil peony 'Fengdan' seeds selected for spare use. It is necessary to ensure that the endosperm is intact after peeling;

[0036] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours...

Embodiment 2

[0042] A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:

[0043] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;

[0044] Step 2. Use needle-nose pliers to carefully remove the outer testa of the reserved peony seeds, making sure that the endosperm is intact after peeling;

[0045] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours. During the soaking period, stir and was...

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for establishing an aseptic culture system for mature seed embryos of peony 'Fengdan' for oil. Background technique

[0002] peony( Paeonia suffruticosa Andr. ) belongs to Paeoniaceae ( Paeoniaeeae ) Paeonia ( Paeonia ) Perennial deciduous subshrubs. 'Feng Dan' ( Paeonia ostii 'Feng Dan' ) is a kind of medical and oil species gradually formed after long-term planting and cultivation of the original species of Yangshan peony. Oil peony 'Fengdan' can be developed and utilized as a new type of woody grain and oil plant resource. Compared with other oil crops, oil peony has the following characteristics: high yield, high oil content, high quality, and low cost. Therefore, vigorously promoting the planting of peony for oil is of great significance to promote the production of grain and oil in my country, change the consumption structure of edible oil an...

Examples

Embodiment 1

[0033]A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:

[0034] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;

[0035] Step 2. Use needle-nose pliers to carefully remove the outer testa of the oil peony 'Fengdan' seeds selected for spare use. It is necessary to ensure that the endosperm is intact after peeling;

[0036] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours...

Embodiment 2

[0042] A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:

[0043] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;

[0044] Step 2. Use needle-nose pliers to carefully remove the outer testa of the reserved peony seeds, making sure that the endosperm is intact after peeling;

[0045] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours. During the soaking period, stir and was...

Images