Method of establishing aseptic culture system for mature seed embryos of oil-use paeonia ostia 'feng dan'
A technology of peony and culture system for oil, applied in the field of plant tissue culture, can solve the problems of high browning rate and high pollution rate, achieve the effects of shortening the dormancy period, reducing pollution rate, and improving application efficiency
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Embodiment 1
[0033]A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:
[0034] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;
[0035] Step 2. Use needle-nose pliers to carefully remove the outer testa of the oil peony 'Fengdan' seeds selected for spare use. It is necessary to ensure that the endosperm is intact after peeling;
[0036] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours...
Embodiment 2
[0042] A method for establishing a high-efficiency aseptic culture system for mature seed embryos of oil peony 'Fengdan', which includes the steps of selecting seeds, removing the outer testa, cleaning and soaking the peeled seeds, disinfecting, removing the endosperm, inoculating and culturing, etc., to achieve The establishment of a high-efficiency aseptic culture system for mature seed embryos of peony 'Fengdan' for oil, the steps are as follows:
[0043] Step 1. From September to December, select healthy mature seeds with plump grains, bright seed coat color and no cracks in the seeds of peony 'Fengdan' newly harvested for oil in August of that year, and set them aside;
[0044] Step 2. Use needle-nose pliers to carefully remove the outer testa of the reserved peony seeds, making sure that the endosperm is intact after peeling;
[0045] Step 3. Wash the seeds with the outer testa removed with distilled water and soak for 24-30 hours. During the soaking period, stir and was...
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