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A molecular marker related to corn grain yield and quality

A molecular marker and corn technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high heritability and complex genetic structure of corn kernels

Active Publication Date: 2022-07-12
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the genetic structure of maize kernel size is complex and controlled by multiple loci, the heritability of maize kernel size is high and there are major genes controlling kernel size

Method used

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  • A molecular marker related to corn grain yield and quality
  • A molecular marker related to corn grain yield and quality
  • A molecular marker related to corn grain yield and quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, chr3.S_215018483 is a SNP related to corn grain yield and quality-related traits

[0044] 1. Discovery of SNPs related to corn grain yield-related traits

[0045] 1. Materials

[0046] Plant material: 141 maize inbred lines, see Table 1.

[0047] 2. Preparation of Samples for Transcriptome Analysis

[0048] In the field, 141 corn inbred lines were planted in a single row of plots, with a row length of 3 meters and a row width of 0.6 meters, and 13 corn plants were planted in each plot. Plants in the plot were bagged before pollination to isolate natural pollen. After more than 80% of the maize plants in the plot spit out filaments, take the pollen and carry out selfing uniformly, and record the pollination time of each plot. On the 15th day after pollination in each plot, 3 relatively consistent fruit ears were taken from each plot, and 5 grains in the middle of each ear were mixed and used as the grain samples of each maize inbred line 15 days after p...

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Abstract

The invention discloses a molecular marker related to corn grain yield and quality. The molecular marker (chr3.S_215018483) disclosed in the present invention is the nucleotide corresponding to the 189th position of sequence 3 in the sequence listing in the maize genome, and the molecular marker is T or C. In maize, the TT genotype corn kernels with chr3.S_215018483 on both chromosomes are T, the grain length and 100-kernel weight are lower than the CC genotype corn with chr3.S_215018483 on both chromosomes, and the TT genotype corn kernels The content of icosic acid was higher than that of CC genotype; the linoleic acid content of TT genotype was lower than that of CC genotype. In breeding, maize of CC genotype can be selected as the parent for breeding to improve grain-related traits—grain length and 100-kernel weight and to increase the linoleic acid content of maize grains.

Description

technical field [0001] The invention relates to a molecular marker related to corn grain yield and quality in the field of biotechnology. Background technique [0002] Maize (Zea mays L.) is a high-yield grain crop that is used for grain, economics and forage. Maize production plays an important role in ensuring global food security. As the world's population continues to increase, human demand for food will increase day by day. However, in the current form of continuous reduction of arable land, increasing the level of yield per unit has become the fundamental way to solve the contradiction between supply and demand of corn. Grain is the main harvesting goal in maize production, and increasing grain yield is the ultimate goal of high-yield maize breeding. Grain weight is one of the three basic elements of corn yield (grain weight, number of grains per ear, number of ears per mu). Therefore, kernel size (such as length, width, weight, etc.) is critical to the improvement...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 崔钰李永祥卢嘉雯陈林张洁李春辉王国英黎裕王天宇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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