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Molecular marker relevant to yield and quality of zea mays grains

A molecular marker, yield-related trait technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high heritability of corn kernel size and complex genetic structure.

Active Publication Date: 2019-12-06
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the genetic structure of maize kernel size is complex and controlled by multiple loci, the heritability of maize kernel size is high and there are major genes controlling kernel size

Method used

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  • Molecular marker relevant to yield and quality of zea mays grains
  • Molecular marker relevant to yield and quality of zea mays grains
  • Molecular marker relevant to yield and quality of zea mays grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, chr3.S_215018483 is a SNP associated with maize grain yield and quality-related traits

[0044] 1. Discovery of SNPs associated with maize grain yield-related traits

[0045] 1. Materials

[0046] Plant material: 141 maize inbred lines, see Table 1.

[0047] 2. Sample Preparation for Transcriptome Analysis

[0048] 141 corn inbred lines were planted in the field, planted in single-row plots with a row length of 3 meters and a row width of 0.6 meters, and 13 corn plants were planted in each plot. Before pollination, the plants in the plot were bagged to isolate natural pollen. After more than 80% of the corn plants in the plot spit out filaments, pollen is collected for selfing, and the pollination time of each plot is recorded. On the day 15 days after pollination in each plot, 3 relatively consistent fruit ears were taken in each plot, and 5 grains in the middle of each ear were taken and mixed as samples of the grains 15 days after pollination of each c...

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Abstract

The invention discloses a molecular marker relevant to yield and quality of zea mays grains. The molecular marker (chr3.S_215018483) disclosed by the invention corresponds to the 189th nucleotide in asequence 3 of a sequence table in a zea mays genome, and is T or C. In zea mays, the grain length and the 100-grain weight of the TT genotype zea mays grains of which the chr3.S_215018483 of two chromosomes is T are both lower than those of CC genotype zea mays of which the chr3.S_215018483 of two chromosomes is C, the arachidic acid content of the TT genotype zea mays grains is higher than thatof the CC genotype zea mays grains, and the linoleic acid content of the TT genotype zea mays grains is lower than that of the CC genotype zea mays grains. During breeding, the CC genotype zea mays can be selected as parents for breeding, so that the grain relevant characters including the grain length and the 100-grain weight are increased, and the linoleic acid content of the zea mays grains isincreased.

Description

technical field [0001] The invention relates to a molecular marker related to corn grain yield and quality in the field of biotechnology. Background technique [0002] Maize (Zea mays L.) is a high-yield grain crop that is used for grain, economics and feed. Maize production plays an important role in ensuring global food security. As the world population continues to increase, human demand for food will increase day by day. However, in the current form of continuous reduction of cultivated land area, increasing the yield per unit area has become the fundamental way to solve the contradiction between supply and demand of corn. Grain is the main harvesting target in maize production, and increasing the grain yield is the ultimate goal of high-yielding maize breeding. Grain weight is one of the three basic elements of corn yield (grain weight, number of grains per ear, number of ears per mu). Therefore, the grain size (such as length, width, weight, etc.) is very important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 崔钰李永祥卢嘉雯陈林张洁李春辉王国英黎裕王天宇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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