Time-resolved wide-spectrum CARS spectral imaging device based on high-repetition-frequency femtosecond laser

Abstract

The invention discloses a time-resolved wide-spectrum CARS spectral imaging device based on high-repetition-frequency femtosecond laser. The device comprises a high-repetition-frequency femtosecond laser device, a double-pulse laser generation module and a spectral imaging platform. The high-repetition-frequency femtosecond laser device is used for generating fundamental frequency light with the high repetition frequency and inputting the fundamental frequency light into the double-pulse laser generation module. The double-pulse laser generation module is used for generating fundamental frequency light and frequency shift light which are suitable for coaxial transmission of the time-resolved CARS imaging technology. And the spectral imaging platform is used for realizing scanning type Raman spectral imaging of a to-be-detected sample. According to the invention, the wide-spectrum femtosecond laser is used as pump light and Stokes light at the same time and coherence among multiple energy levels can be established at one time, so that hyperspectral imaging of various molecules can be realized at the same time. A coherent excitation process and a detection light excitation process which are jointly realized by the pump light and Stokes light are separated in time and narrow-spectrum light is adopted as the pump light, so that a non-resonance background is effectively suppressed,and the detection sensitivity is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and more specifically relates to a time-resolved wide-spectrum CARS spectral imaging device based on a high repetition frequency femtosecond laser. Background technique [0002] Optical imaging technology has a wide range of applications in materials science, biomedicine and other fields. At present, there are still many problems in the traditional optical imaging technology. For example, general optical imaging lacks chemical specificity and cannot image the spatial distribution of a specific molecule in a sample. This lack of chemical information will largely limit the application of imaging technology; although fluorescent labeling imaging has chemical specificity However, specific molecules need to be labeled, that is, complex pretreatment of the sample is required, which is not only time-consuming and laborious, but may also change the characteristics of the sample. Moreover, there ...

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Problems solved by technology

[0005] Aiming at the defects of the prior art, the purpose of the present invention is to provide a time-resolved wide-spectrum CARS spectral imaging device based on a high repetition frequency femtosecond laser, aiming at solving the difficulty of traditional CARS spectral imaging technology in maintaining a simple system structure and low cost At the same time, realize single-time detection of multiple molecules, and effectively suppress the problem of non-resonance background

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