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Screening and application of core primers for eggplant ssr molecular markers

A core primer and molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of narrow genetic basis and large genetic distance, and achieve clear amplification bands. Effect

Active Publication Date: 2022-06-24
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Lu Ting et al. analyzed the genetic diversity of 66 eggplant materials with 25 pairs of SSR primers, and concluded that the genetic distance between eggplant species is relatively large, and the genetic basis within cultivated species is relatively narrow.

Method used

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  • Screening and application of core primers for eggplant ssr molecular markers
  • Screening and application of core primers for eggplant ssr molecular markers
  • Screening and application of core primers for eggplant ssr molecular markers

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Embodiment

[0030] The present embodiment relates to the eggplant SSR molecular marker core primer and its screening, and the application in the identification of the kinship relationship of the variety; Specifically as follows:

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] The 133 pairs of SSR primers used in this experiment were synthesized by Shanghai Sangon Biotechnology Co., Ltd. In order to screen primers with higher polymorphism from 133 pairs of primers, 106 eggplant varieties were selected according to fruit shape, fruit edge (yes / absence), peel color (white / green / purple), fruit surface stripe, leaf 6 traits of color and longitudinal diameter of fruit selected 12 varieties with large differences (see Table 2) to screen SSR primers. The research materials for primer verification and relationship analysis were 106 eggplant germplasm samples (see Table 1), all from the Shanghai Branch of the New Plant Variety Testing Center of the Ministry of Agriculture and Rur...

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Abstract

The invention discloses screening and application of eggplant SSR molecular marker core primers; the eggplant SSR molecular marker core primer set includes 17 pairs of SSR molecular marker core primer pairs. The present invention screens out eggplant SSR primers that are stable, reliable, and highly polymorphic by extracting the DNA and SSR-PCR of eggplant core germplasms with large differences in traits; 106 eggplant cultivar resources are paired with 17 pairs of SSR primers screened by the present invention The analysis was carried out; the similarity calculation and cluster analysis showed that the 17 pairs of primers screened could identify eggplant varieties accurately and efficiently; and then laid the foundation for the application of SSR molecular marker technology in eggplant germplasm genetic relationship analysis and variety identification.

Description

technical field [0001] The invention belongs to the technical field of molecular marker technology development and application, and in particular relates to the screening and application of a core primer for eggplant SSR molecular marker. Background technique [0002] Eggplant (Solanum melongene L.) is a crop of the genus Solanum (Solanum L.) of the family Solanaceae. The number of chromosomes is 2n=2x=24. Its fruit is a berry, and it likes high temperature. Eggplants were called cheese crisps and Kunlun melons in ancient times, commonly known as scallops, dwarf melons, and rosu. They are annual herbs and perennial shrubs in the tropics. Eggplant originated in the tropical region of southern Asia. Ancient India was the earliest domestication place. China has a long history of cultivating eggplant and is the second place of origin of eggplant. It not only has many varieties, but also has abundant germplasm resources. [0003] Eggplant is an important vegetable in my country ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11C12Q1/6806
CPCC12Q1/6895C12Q1/6806C12Q2600/13C12Q2600/156C12Q2531/113
Inventor 邓姗任丽陈海荣章毅颖褚云霞赵洪李寿国
Owner SHANGHAI ACAD OF AGRI SCI
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