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Application of Fusarium proliferatum in Prevention and Control of Invasive Plant Xanthium strumarium

A technology for invading plants and Fusarium, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of plant killing, chemical control and animal harm, etc.

Inactive Publication Date: 2020-01-14
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mechanical eradication can only work in a short period of time and in a small area, and it is time-consuming and laborious; chemical control does not have good specificity and selectivity, it will kill other plants, and may also cause harm to animals in the habitat. , due to the lack of microorganisms to decompose it in nature, it will pollute the environment; and fungal herbicides can be degraded by microorganisms because they are derived from nature, which is more friendly to the environment and meets the needs of sustainable development

Method used

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  • Application of Fusarium proliferatum in Prevention and Control of Invasive Plant Xanthium strumarium
  • Application of Fusarium proliferatum in Prevention and Control of Invasive Plant Xanthium strumarium

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The preparation method of layered Fusarium mycelium:

[0034] First, inoculate the bacteria into two 1000mL Erlenmeyer flasks containing 400mL of sterilized PDB medium, and cultivate them in a constant temperature culture shaker at 160rpm at a temperature of 28°C to form a bacterial liquid. Visible bacteria will form after 3 days The seed solution of the block was inoculated in the rice medium of 60 1000mL Erlenmeyer flasks for amplified cultivation, each bottle contained 200g rice, 200mL distilled water, sterilized at 121°C for 30min, each bottle received 10mL seed solution, and cultured at room temperature for 10 sky. After the end, the rice and the thalline are mechanically pulverized to obtain the mycelia of Fusarium laminarum;

[0035] The mass ratio of the prepared pathogen mycelia to water was dissolved at 1:10, and sprayed on the leaf surface of the invasive plant Xanthia coxanthii at 20 g / mu. After 5 days, obvious lesion appeared on the leaves, and then the di...

Embodiment 2

[0037] The preparation method of layered Fusarium mycelium:

[0038] First, inoculate the bacteria into two 1000mL Erlenmeyer flasks containing 400mL of sterilized PDB medium, and cultivate them in a constant temperature culture shaker at 160rpm at a temperature of 28°C to form a bacterial liquid. Visible bacteria will form after 3 days The seed solution of the block was inoculated in the rice medium of 60 1000mL Erlenmeyer flasks for amplified cultivation, each bottle contained 200g rice, 200mL distilled water, sterilized at 121°C for 30min, each bottle received 10mL seed solution, and cultured at room temperature for 10 days, after the end, the rice and the thalline were mechanically pulverized to obtain the layered Fusarium hyphae;

[0039] The mass ratio of the prepared pathogen mycelia to water is dissolved at 1:5, and sprayed on the invasive plant cocklebur leaf surface by 60g / mu. After 3 days, obvious scabs appear on the blade, and then the condition worsens, and the bl...

Embodiment 3

[0041] The preparation method of layered Fusarium mycelium:

[0042] First, inoculate the bacteria into two 1000mL Erlenmeyer flasks containing 400mL of sterilized PDB medium, and cultivate them in a constant temperature culture shaker at 160rpm at a temperature of 28°C to form a bacterial liquid. Visible bacteria will form after 3 days The seed solution of the block was inoculated in the rice medium of 60 1000mL Erlenmeyer flasks for amplified cultivation, each bottle contained 200g rice, 200mL distilled water, sterilized at 121°C for 30min, each bottle received 10mL seed solution, and cultured at room temperature for 10 days, after the end, the rice and the thalline were mechanically pulverized to obtain the layered Fusarium hyphae;

[0043] The mass ratio of the prepared pathogen mycelia to water was dissolved at 1:8, and sprayed on the leaf surface of the invasive plant Xanthia coxanthii at 100 g / mu. After 1 day, obvious lesion appeared on the leaves, and then the disease ...

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Abstract

The invention relates to an application of Fusarium proliferatum in preventing and controlling invasive plant Xanthium strumarium, the pathogenic bacteria strain is classified and named as Fusarium proliferatum (Fusarium proliferatum), and is isolated and purified from the leaves of Xanthium strumarium naturally diseased plants near farmland in the suburbs of Changji City, Xinjiang. Pathogenic mycelium and crude toxin are prepared by conventional methods from the isolated and purified Fusarium proliferatum, and then the prepared pathogenic mycelium and crude toxin are sprayed on Xanthium strumarium and have strong control effect on invasive plant Xanthium strumarium. According to the invention, the culture operation is simple, the culture matrix material is easy to obtain, the obtained pathogenic bacteria hyphae and crude toxin have strong stability, the acquisition process is simple, mass production can be carried out, the production period is short, and the popularization and application are easy; by spraying on stems and leaves, it is convenient to use, can effectively kill the target invading plant Xanthium strumarium, and has higher environmental safety.

Description

technical field [0001] The invention belongs to the field of plant protection, and specifically relates to the application of layered Fusarium fungus in preventing and controlling invasive plant cocklebur. Background technique [0002] Biological invasion refers to the non-indigenous species that stay alive and reproduce after arriving in a new area, and then form a population of a certain scale. The continuous spread of this population will have a significant impact on the local environment and economy. Species that lead to biological invasion are called Invasive alien species (IAS for short). Invasive species will directly or indirectly reduce the species diversity in the area, and its impact is only weaker than the loss of species habitat. It can make the composition and function of ecosystems in different regions tend to be uniform, and eventually degrade and lose stable ecology System functions. In recent years, because invasive organisms have a significant impact on ...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N61/00A01P3/00C12N1/14C12R1/77
CPCA01N61/00C12N1/14
Inventor 张晨鹏邵华骆世洪张弛刘铁韩彩霞周世兴
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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