A temperature conduction system for nucleic acid amplification instrument

A technology of conduction system and amplifying instrument, which is applied in laboratory containers, bioreactors/fermenters for specific purposes, biochemical instruments, etc., can solve the problems of low temperature control efficiency and insufficient temperature control accuracy, and improve the Temperature control accuracy, improve temperature control efficiency, and achieve the effect of precise control

Active Publication Date: 2021-03-19
宁波胤瑞生物医学仪器有限责任公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of low temperature control efficiency and insufficient temperature control accuracy of the existing PCR instrument control system, the present invention provides a brand new temperature conduction system, which can realize rapid and high-precision temperature control

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  • A temperature conduction system for nucleic acid amplification instrument
  • A temperature conduction system for nucleic acid amplification instrument

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Embodiment Construction

[0031] The temperature conduction system for a nucleic acid amplification instrument provided by the present invention will be specifically described below in combination with specific embodiments. It is evident that practice of the embodiments of the invention is not limited to specific details familiar to those skilled in the art. Preferred embodiments of the present invention are described in detail below, however, the present invention may have other embodiments besides these detailed descriptions.

[0032]It should be noted that: the system set temperature T1' in the present invention is the temperature set on the PCR instrument, and corresponding to each set temperature, the PCR instrument can automatically calculate the temperature of each equipment (the first refrigerating sheet 3, the second refrigerating sheet 4 1. Heat sink 5) The initial temperature that needs to be adjusted to, which is the set temperature of each device. However, in the actual control process, d...

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Abstract

The invention provides a temperature conduction system for a nucleic acid amplifier. The temperature conduction system comprises a fan module, a temperature detection module, a control module and a fan module chip support, a chip groove is formed in the chip support, a chip is installed in the chip groove, a heating cover covers the chip support, and a first refrigeration sheet, a second refrigeration sheet, a cooling fin and a fan are sequentially arranged below the chip. The control module is connected with the temperature detection module and the fan module and used for receiving the temperature information sent by the temperature detection module and controlling temperature rising and falling of the heating cover, the first refrigeration sheet, the second refrigeration sheet and the cooling fin and the rotating speed of the fan. The invention provides the temperature conduction system, according to the invention, the temperature change of each device can be detected in real time, which device needs to be subjected to temperature adjustment is analyzed in a layer-by-layer judgment manner, all devices do not need to be adjusted, the adjustment control difficulty can be greatly reduced, and the chip temperature can be accurately controlled, so that the temperature control precision and the temperature control efficiency can be improved.

Description

technical field [0001] The invention relates to the field of PCR temperature control, in particular to a temperature conduction system for a nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification technology, especially digital polymerase chain reaction technology (Polymerase Chain Reaction referred to as PCR), is a molecular biological technology used to amplify specific DNA molecular fragments. It uses DNA molecules as templates and is artificially synthesized by a pair of The specific oligonucleotide primers can quickly amplify specific DNA molecular fragments through the enzymatic reaction of DNA polymerase, which plays an extremely important role in biology. The basic process of PCR reaction is divided into three steps. The first step is DNA denaturation (94°C), and the hydrogen bond of the double-stranded DNA template is broken under the action of heat to form single-stranded DNA; the second step is annealing (55°C), the tempera...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L3/5027B01L7/52B01L2300/0887
Inventor 沈小骞任鲁风隋硕张未来俞育德于军
Owner 宁波胤瑞生物医学仪器有限责任公司
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