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A kind of microorganism isolation culture and culture liquid supply device

A technology for supplying device and culture medium, applied in enzymology/microbiology device, biochemical cleaning device, method for supporting/fixing microorganisms, etc., can solve problems such as affecting the normal growth of microorganisms, slow dialysis speed, and easily damaged membrane, etc. To achieve the effect of benefiting growth and reproduction, reducing the amount of filtration, and increasing the uniformity of distribution

Active Publication Date: 2022-07-29
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a microbial isolation culture and culture solution supply device, to solve the problems of easy damage of the membrane and slow dialysis speed in the above-mentioned background technology, and to solve the problem of separating the culture solution while affecting the normal growth of microorganisms

Method used

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  • A kind of microorganism isolation culture and culture liquid supply device
  • A kind of microorganism isolation culture and culture liquid supply device

Examples

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Embodiment 1

[0038] like figure 1 , a microbial isolation culture and culture solution supply device of this embodiment, this embodiment takes Acidithiobacillus ferrooxidans as an example to cultivate, and the main nutrient is Fe 2+ , the main metabolite is Fe 3+ , this example uses Fe 3+ Oxidation of metal to achieve removal of metal materials, Fe 3+ Self-reduction to Fe while oxidizing metals 2+ , so the processed liquid can be recycled. The 6 types of microorganisms to which this embodiment is applicable are not limited to the cultivation of fungi and algae, but are also applicable to any other suitable types of microorganisms 6 . The term "culture liquid" refers to the liquid in which the target microorganism 6 is cultivated, and includes water, nutrients required for the cultivation of the microorganism 6, metabolites of the microorganism 6, and the like.

[0039] A microorganism isolation culture and culture solution supply device in this embodiment includes a bioreactor 1, a cu...

Embodiment 2

[0055] The difference between Embodiment 2 and Embodiment 1 is that this embodiment also includes a liquid replenishing device 8, which is filled with an appropriate amount of sucrose culture medium for supplementing the culture solution consumed in the process of culturing microorganisms.

[0056] like figure 2 , the microbial species cultivated in the present embodiment is Saccharomyces cerevisiae, the main nutrient is sucrose, and the main metabolite is alcohol. The present embodiment separates and (or) purifies the culture solution and finally obtains an alcohol product. Sucrose medium needs to be supplemented with alcohol. In the present embodiment, the supply form is non-recyclable, so the post-processing device 7 is not provided with a return line to the culture medium exchanger 2, and the separate culture and supply methods are as follows:

[0057] a. Prepare a certain volume of yeast inoculum in logarithmic growth phase and a liquid yeast fermentation medium with 4 ...

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Abstract

The invention discloses a microorganism isolation culture and culture liquid supply device, which comprises a biological reactor, a culture liquid exchanger and a hydraulic adjustment pump. A filter membrane or filter element is used to surround the microorganisms in the culture medium space of the culture medium exchanger, and the other part of the culture medium does not contain microorganisms, so as to realize the isolation culture, and the positive and negative cycle changes are generated on both sides of the filter membrane or the filter element through the hydraulic adjustment pump. The pressure difference forces the culture medium to flow back and forth through the filter membrane or filter element, increasing the exchange rate of nutrients and metabolites in the separated space. The culture solution on the side without microorganisms is transported to the designated place by the culture solution delivery pump. Finally, the normal growth of microorganisms is not affected while separating the culture medium.

Description

technical field [0001] The invention belongs to the field of microorganism culture, and in particular relates to a microorganism isolation culture and culture liquid supply device. Background technique [0002] With the continuous development of industrial technology, especially microbial engineering, the application depth and breadth of microorganisms in industrial production have been improved to a certain extent. In these applications, it is not the microbial cell body itself that is used in many cases, but the metabolites of the microorganism. Therefore, in many cases, it is necessary to separate the culture solution from the microbial cell population to obtain a culture solution containing metabolites, which satisfies industrial applications. However, the common filtration separation method is not only time-consuming and labor-intensive, but also affects the normal growth of microorganisms and reduces the yield of metabolites. In order to obtain the culture medium con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/12
CPCC12M25/00C12M25/02C12M29/04C12M29/20C12M41/22C12M47/10
Inventor 黄辉马飞
Owner HUAQIAO UNIVERSITY
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