Culture method for culturing desulfovibrio

Abstract

The invention relates to a culture method for culturing desulfovibrio. The culture method comprises the following steps: firstly, putting a prepared culture medium into a petri dish, horizontally placing the petri dish on a mechanical self-leveling petri dish rack, and performing leveling; secondly, adding diluents of different concentrations into the petri dish, performing inoculation by using astreak plate method, adding a culture glue, and ensuring an anaerobic environment; and finally, in order to increase inoculation areas and inoculation numbers, performing inclined culture on the petridish, adjusting the angle between the hammer of the mechanical self-leveling petri dish rack and a support plate to an appropriate angle, and performing leveling so as to prevent the petri dish fromsliding off. The method provided by the invention is very simple and convenient, applicable to sealed culture of the desulfovibrio by using the culture glue, is capable of reducing the influence of imbalance of the petri dish rack or operation of an operator to the sealing effect of the petri dish, in addition, is applicable to inclined culture on the desulfovibrio, and is capable of increasing culture areas and inoculation numbers, achieving automatic leveling of the petri dish in the inclined culture process and ensuring inclined culture of the petri dish to achieve the sealing effect.

Description

technical field [0001] The invention belongs to a method for cultivating Desulfovibrio, in particular to a method for cultivating Desulfovibrio. Background technique [0002] At present, there are many domestic and foreign methods for the purification of Desulfovibrio culture, but all of them require auxiliary equipment and a large number of test consumables during the culture process. Generally, it can be divided into two categories. The first category is that during the cultivation process of Desulfovibrio, a closed container is required, and a special agent (oxygen absorber) is added to the container; the second method requires large-scale equipment or equipment. CO is continuously introduced in the process 2 To isolate the oxygen in the external environment (such as CO 2 Incubator), the above method requires certain laboratory conditions to cultivate Desulfovibrio, and the resource consumption in the cultivation process is relatively large. All of these are based on t...

AI Technical Summary

Problems solved by technology

However, the use of culture fluid for sealing has the problem of poor sealing effect.

[0003] The deficiencies in the cultivation process of the culturing method of cultivating Desulfovibrio with culture fluid sealed in the prior art are that the culture fluid is tilted due to the uneven ground of the culture dish rack or the inclination of the operator, resulting in oxygen infiltration and sealing failure. Affect Desulfovibrio culture

At the same time, when the operator places and removes other petri dishes from the petri dish rack, the vibration will affect the sealing effect of this Desulfovibrio petri dish, causing the seal to fail

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