A kind of nano hybrid silk fibroin hydrogel and its preparation method and application
A technology for silk fibroin and regenerating silk fibroin, which is applied in the field of nano-hybrid silk fibroin hydrogel and its preparation, which can solve the problem of unfavorable cell or biological factor viability, ranging from days to weeks, gel It can achieve the effect of excellent induction of stem cell osteogenic differentiation, good vitality, and maintenance of vitality.
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Embodiment 1
[0069] A kind of preparation method of nanoscale hydroxyapatite hybrid silk fibroin hydrogel, such as figure 2 shown, including the following steps:
[0070] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30 minutes, repeat twice, wash with pure water several times, tear loose, and dry at 40°C. Weigh the dried silk protein at a concentration of 15% by mass fraction, dissolve it at 60°C for 2 hours with a LiBr solution with a concentration of 9.3M, then put it into a dialysis bag with a molecular weight cut-off of 3500, and dialyze it with pure water at 4°C for three days. During this period, the water was changed every 6 hours, and the insoluble precipitate was removed by gauze filtration to obtain an aqueous solution of regenerated silk fibroin; it was stored in a refrigerator at 4°C.
[0071] (2) Disperse the nano-sized hydroxyapatite in the pure aqueous solution according to the concentration of...
Embodiment 2
[0078] A preparation method of nano-hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 shown, including the following steps:
[0079] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30min, repeat twice, wash with pure water several times, and dry at 40°C; The 9.3M LiBr solution was dissolved at 60°C for 1 hour, so that the mass fraction of silk protein in the dissolved mixed solution was 15%, and then put into a dialysis bag with a molecular weight cut-off of 3500, and dialyzed in pure water at 10°C for three days. The polyethylene glycol solution (molecular weight is 20,000 Da) of %wt concentrates the regenerated silk fibroin aqueous solution after dialysis into the regenerated silk fibroin aqueous solution of 6%wt (determined by dry weighing method), removes insoluble precipitate by gauze filtration, Store in refrigerator at 4°C.
[0080] (2) Disperse nano-sized hydroxyapatite ...
Embodiment 3
[0085] A preparation method of nano-hybrid silk fibroin hydrogel for bone defect repair, such as figure 2 shown, including the following steps:
[0086] (1) According to the bath ratio of 1:50 (mass ratio), add silkworm cocoons into 0.02M sodium carbonate solution and boil for 30min, repeat twice, wash with pure water several times, and dry at 40°C; The 9.3M LiBr solution was dissolved at 60°C for 1 hour, so that the mass fraction of silk protein in the dissolved mixed solution was 15%, and then put into a dialysis bag with a molecular weight cut-off of 3500, and dialyzed in pure water at 10°C for three days. %wt polyethylene glycol solution (molecular weight: 20,000 Da) to concentrate the dialyzed regenerated silk fibroin aqueous solution into a 6%wt regenerated silk fibroin aqueous solution, filter through gauze to remove insoluble precipitates, and store in a refrigerator at 4°C.
[0087] (2) Disperse nano-sized hydroxyapatite in pure aqueous solution according to the con...
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