Urine 5-hydroxyindoleacetic acid detection reagent and preparation method thereof

A technology of oxindole acetic acid and detection reagent, which is applied to measurement devices, material analysis, instruments and other directions by observing the influence on chemical indicators, can solve the problems of complex urine composition, false positives, misdiagnosis, etc., and achieves sensitivity and High accuracy, preventing false positives, and strong specificity

Inactive Publication Date: 2020-03-31
欧迪玛(湖北)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Colorimetry is currently the most commonly used method for detecting 5-hydroxyindoleacetic acid in urine. The detection reagent can react with 5-hydroxyindoleacetic acid in human urine, and the excretion of human metabolites is affected by many physiological or The influence of pathological factors makes the composition of urine complex. Some trace components or abnormal metabolic products caused by other causes will also combine with mercury ions and mercury ions in the detection reagent to form color precipitates, which will seriously interfere with the accuracy of the test results. The problem of false positives occurs, causing misdiagnosis and limiting its application value

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  • Urine 5-hydroxyindoleacetic acid detection reagent and preparation method thereof
  • Urine 5-hydroxyindoleacetic acid detection reagent and preparation method thereof
  • Urine 5-hydroxyindoleacetic acid detection reagent and preparation method thereof

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Embodiment 1

[0030] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.12mol / L, the concentration of mercurous nitrate is 0.75mol / L, the concentration of zinc nitrate is 0.36mmol / L, the concentration of phosphomolybdic acid is 0.75mmol / L, and the concentration of silicotungstic acid is 4.17mmol / L.

[0031] The preparation method of the above-mentioned detection reagent comprises the following steps:

[0032] S1. Preparation of mixed acid solution: mix concentrated nitric acid and concentrated phosphoric acid in equal volumes, add water to dilute to 3 times the volume of the mixture, and cool to room temperature for later use;

[0033] S2, preparation of A solution: take mercuric nitrate and dissolve it in the mixed acid solution described in step...

Embodiment 2

[0040] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.31mol / L, the concentration of mercurous nitrate is 0.56mol / L, the concentration of zinc nitrate is 1.27mmol / L, the concentration of phosphomolybdic acid is 2.81mmol / L, and the concentration of silicotungstic acid is 7.82mmol / L.

[0041] The preparation method of the above-mentioned detection reagent comprises the following steps:

[0042] S1. Preparation of mixed acid solution: mix concentrated nitric acid and concentrated phosphoric acid in equal volumes, add water to dilute to 4 times the volume of the mixture, and cool to room temperature for later use;

[0043] S2, the preparation of A solution: take by weighing mercuric nitrate and dissolve in the mixed acid solution desc...

Embodiment 3

[0050] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.20mol / L, the concentration of mercurous nitrate is 1.07mol / L, the concentration of zinc nitrate is 0.25mmol / L, the concentration of phosphomolybdic acid is 1.46mmol / L, and the concentration of silicotungstic acid is 5.70mmol / L.

[0051] The preparation method of the above-mentioned detection reagent comprises the following steps:

[0052] S1. Preparation of mixed acid solution: mix equal volumes of concentrated nitric acid and concentrated phosphoric acid, add water to dilute to 5 times the volume of the mixture, and cool to room temperature for later use;

[0053] S2, preparation of A solution: take mercuric nitrate and dissolve in the mixed acid solution described in step S1...

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Abstract

The invention discloses a urine 5-hydroxyindoleacetic acid detection reagent. The detection reagent is an aqueous solution containing nitric acid, phosphoric acid, mercury nitrate, mercurous nitrate,zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of the mercury nitrate is 0.12 mol / L to 0.35 mol / L, the concentration of the mercurous nitrate is 0.56 to 1.15 mol / L, the concentration of the zinc nitrate is 0.25 to 1.37 mmol / L, the concentration of the phosphomolybdic acid is 0.63 to 3.10 mmol / L, and the concentration of the silicotungstic acid is 2.19 to 8.20mmol / L. The invention further provides a preparation method of the urine 5-hydroxyindoleacetic acid detection reagent. The urine 5-hydroxyindoleacetic acid detection reagent is prepared by mixing theprepared mercury nitrate solution, mercurous nitrate solution, zinc nitrate solution, phosphomolybdic acid solution and silicotungstic acid solution according to a certain proportion. The urine 5-hydroxyindoleacetic acid detection reagent disclosed by the invention is simple in preparation process, high in detection result accuracy, good in specificity and convenient and rapid in detection process, has universality when being used for cancer detection, and is suitable for large-scale popularization.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a urine 5-hydroxyindoleacetic acid detection reagent and a preparation method thereof. Background technique [0002] According to the World Health Organization's 2018 data, cancer has become the second leading cause of death in the world, and nearly one in six deaths is caused by cancer. According to the forecast of the International Agency for Research on Cancer, if no effective measures are taken, the number of cancer cases and deaths in my country will rise to 4 million and 3 million by 2020; and will rise to 5 million and 3.5 million by 2030. So far, countries around the world have invested a lot of manpower and material resources in the prevention, diagnosis and treatment of tumors. Early diagnosis and treatment are of vital significance to improve the survival rate of cancer patients. Commonly used clinical imaging methods such as X-ray, ultrasound, PET-CT, and magneti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/82
CPCG01N21/78G01N21/82G01N2021/775
Inventor 郭东辉
Owner 欧迪玛(湖北)生物科技有限公司
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