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Mesenchymal stem cell culture medium

A stem cell and stem cell technology, applied in the field of stem cell culture and mesenchymal stem cell culture medium, can solve the problems of stem cell aging, discount of disease treatment effect, and reduced multi-directional differentiation ability.

Inactive Publication Date: 2020-04-03
南京温博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have shown that excessive long-term subculture in vitro will cause aging of stem cells, reduce multidirectional differentiation ability, and greatly reduce the therapeutic effect on diseases

Method used

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  • Mesenchymal stem cell culture medium
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  • Mesenchymal stem cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Anti-aging effect of pullulan on human amniotic mesenchymal stem cells

[0017] 1. Experimental materials

[0018] D-Hanks solution was purchased from Guangzhou Peiyu Biological Products Co., Ltd.

[0019] Trypsin was purchased from Shanghai Hengdu Biotechnology Co., Ltd.

[0020] Collagenase type II was purchased from Abisson (Shanghai) Biotechnology Co., Ltd.

[0021] DMEM / F12 medium, FBS and penicillin and streptomycin were purchased from Gibco.

[0022] Pullulan (CAS No. 9057-02-7) was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with a purity of ≥99%.

[0023] The fluorescently labeled flow antibodies used in flow cytometric identification are products of Beckman.

[0024] The β-galactosidase staining kit was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0025] 2. Experimental method

[0026] 1. Cell isolation, culture and identification

[0027] Peel off the amniotic membrane on the fetal surface of the placenta under asepti...

Embodiment 2

[0050] Example 2: Human amniotic mesenchymal stem cell culture medium

[0051] A cell culture medium for delaying the aging of human amniotic mesenchymal stem cells is prepared by adding an effective concentration of pullulan to DMEM / F12 medium.

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Abstract

The invention discloses a mesenchymal stem cell culture medium. The researches show that excessive in-vitro long-term subculture can cause aging of stem cells and reduction of multidirectional differentiation capacity, and the treatment effect on diseases is greatly reduced. The pullulan can reduce the aging degree of the human amniotic mesenchymal stem cells, but does not obviously influence thestem cell characteristics of the human amniotic mesenchymal stem cells and does not influence the multidirectional differentiation capacity of the human amniotic mesenchymal stem cells. Therefore, pullulan with effective concentration can be added into a human amniotic mesenchymal stem cell culture medium to prepare the culture medium for resisting aging of the human amniotic mesenchymal stem cells.

Description

technical field [0001] The invention belongs to the biological field and relates to stem cell culture, in particular to a mesenchymal stem cell culture medium. Background technique [0002] Amniotic mesenchymal stem cells are a type of stem cells derived from amniotic membrane. Compared with stem cells from other sources, amniotic mesenchymal stem cells have low immunogenicity and immunosuppressive characteristics, which makes the transplantation of amniotic mesenchymal stem cells have less side effects. Amniotic mesenchymal stem cell transplantation has achieved good results in the treatment of some inflammation-related diseases, and relevant clinical trials are being carried out gradually. [0003] The use of amniotic mesenchymal stem cells to treat diseases inevitably involves short-term or long-term subculture in vitro. However, studies have shown that excessive long-term subculture in vitro will cause aging of stem cells, lower multidirectional differentiation ability...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/34
Inventor 姜东花其他发明人请求不公开姓名
Owner 南京温博生物科技有限公司
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