Mesenchymal stem cell culture medium
A stem cell and stem cell technology, applied in the field of stem cell culture and mesenchymal stem cell culture medium, can solve the problems of stem cell aging, discount of disease treatment effect, and reduced multi-directional differentiation ability.
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[0016] Example 1: Anti-aging effect of pullulan on human amniotic mesenchymal stem cells
[0017] 1. Experimental materials
[0018] D-Hanks liquid was purchased from Guangzhou Peiyu Biological Products Co., Ltd.
[0019] Trypsin was purchased from Shanghai Hengdu Biotechnology Co., Ltd.
[0020] Type II collagenase was purchased from Aibixin (Shanghai) Biotechnology Co., Ltd.
[0021] DMEM / F12 medium, FBS and penicillin were purchased from Gibco.
[0022] Pullulan (CAS No. 9057-02-7) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a purity of ≥99%.
[0023] The fluorescently-labeled flow-through antibodies used in the cytometric identification were products of Beckman Company.
[0024] β-Galactosidase staining kit was purchased from Shanghai Yuanye Biotechnology Co., Ltd.
[0025] 2. Experimental method
[0026] 1. Cell isolation, culture and identification
[0027] The placenta and fetal face amniotic membrane were peeled off under sterile conditions, ...
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[0050] Example 2: Human Amniotic Mesenchymal Stem Cell Culture Medium
[0051] A cell culture medium for delaying the aging of human amniotic mesenchymal stem cells is prepared by adding an effective concentration of pullulan to a DMEM / F12 medium.
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