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Method for rejuvenated culture of rhubarb young seedlings

A technology for seedlings and rhubarb, applied in the field of rhubarb seedling rejuvenation, can solve the problems of seedling system resistance and growth speed that cannot quickly enter the growth recovery period, cannot meet the needs of rejuvenation, and seedlings grow slowly, etc., to improve the seedling system Effects of resistance, promotion of photosynthesis and promotion of seedling growth

Pending Publication Date: 2020-04-17
甘肃步云农牧科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The potato virus-free tissue-cultured seedlings cultivated by this method grow well, have strong rhizomes, tenacious vitality, and enhance the drought-resistant ability of the tissue-cultured seedlings. However, this method uses sucrose water pre-cultivation, and only cultivates the roots of the seedlings, and does not affect the stems and leaves. Although the rejuvenation cultivation can improve the quality of the seedlings, it still cannot meet the rejuvenation requirements, and after transplanting, the seedling system resistance and growth speed cannot quickly enter the growth recovery period, and the seedlings grow slowly during the cultivation process
In addition, only the roots of seedlings are treated, and there are certain limitations

Method used

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  • Method for rejuvenated culture of rhubarb young seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] One, prepare strong seedling culture medium, comprise the steps:

[0029] 1) Prepare raw materials according to the following ratio:

[0030] Diatomaceous earth 1-3g, rice wheat stone 1-2g, swamp mud 50-80g, mud 1000L;

[0031] Macroelements: NH 4 NO 3 1.2g / L, KNO 3 1.5g / L, CaCl 2 4H 2 O 0.35g / L, MgSO 4 ·7H 2 O 0.25g / L, KH 2 PO 4 0.12g / L;

[0032] Trace elements: KI0.25mg / L, H 3 BO 3 1.2mg / L, MnSO 4 5.8mg / L, ZnSO 4 2.4mg / L, Na 2 MoO 4 0.05mg / L, CuSO 4 0.01mg / L, CoCl 2 ·6H 2 O 0.01mg / L, FeSO 4 ·7H 2 O 9.8mg / L, CrCl 3 ·6H 2 O0.01mg / L;

[0033] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0034] Organic ingredients: inositol 25mg / L, sodium benzoate 24-25mg / L;

[0035] Others: sucrose 20g / L, appropriate amount of deionized water.

[0036] 2) Dissolve macroelements, trace elements, growth regulators and sucrose in sterile water at 40-45°C, mix well, add sodium α-naphthalene acetate and sodium benzoate, cool down to 25-30°C, mix under high...

Embodiment 2

[0047] One, prepare strong seedling culture medium, comprise the steps:

[0048] 1) Prepare raw materials according to the following ratio:

[0049] Diatomaceous earth 1-3g, rice wheat stone 1-2g, swamp mud 50-80g, mud 1000L;

[0050] Macroelements: NH 4 NO 3 1.2g / L, KNO 3 1.5g / L, CaCl 2 4H 2 O 0.35g / L, MgSO 4 ·7H 2 O 0.25g / L, KH 2 PO 4 0.12g / L;

[0051] Trace elements: KI0.25mg / L, H 3 BO 3 1.2mg / L, MnSO 4 5.8mg / L, ZnSO 4 2.4mg / L, Na 2 MoO 4 0.05mg / L, CuSO 4 0.01mg / L, CoCl 2 ·6H 2 O 0.01mg / L, FeSO 4 ·7H 2 O 9.8mg / L, CrCl 3 ·6H 2 O0.01mg / L;

[0052] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0053] Organic ingredients: inositol 25mg / L, sodium benzoate 24-25mg / L;

[0054] Others: sucrose 20g / L, appropriate amount of deionized water.

[0055] 2) Dissolve macroelements, trace elements, growth regulators and sucrose in sterile water at 40-45°C, mix well, add sodium α-naphthalene acetate and sodium benzoate, cool down to 25-30°C, mix under hi...

Embodiment 3

[0066] One, prepare strong seedling culture medium, comprise the steps:

[0067] 1) Prepare raw materials according to the following ratio:

[0068] Diatomaceous earth 1-3g, rice wheat stone 1-2g, swamp mud 50-80g, mud 1000L;

[0069] Macroelements: NH 4 NO 3 1.2g / L, KNO 3 1.5g / L, CaCl 2 4H 2 O 0.35g / L, MgSO 4 ·7H 2 O 0.25g / L, KH 2 PO 4 0.12g / L;

[0070] Trace elements: KI0.25mg / L, H 3 BO 3 1.2mg / L, MnSO 4 5.8mg / L, ZnSO 4 2.4mg / L, Na 2 MoO 4 0.05mg / L, CuSO 4 0.01mg / L, CoCl 2 ·6H 2 O 0.01mg / L, FeSO 4 ·7H 2 O 9.8mg / L, CrCl 3 ·6H 2 O0.01mg / L;

[0071] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0072] Organic ingredients: inositol 25mg / L, sodium benzoate 24-25mg / L;

[0073] Others: sucrose 20g / L, appropriate amount of deionized water.

[0074] 2) Dissolve macroelements, trace elements, growth regulators and sucrose in sterile water at 40-45°C, mix well, add sodium α-naphthalene acetate and sodium benzoate, cool down to 25-30°C, mix under hi...

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Abstract

The invention discloses a method for rejuvenated culture of rhubarb young seedlings. The method comprises the steps: submerging roots of the rhubarb young seedlings into sterile digested effluent, carrying out activating treatment for 7 to 14 days under the conditions that a temperature is controlled to 20 DEG C to 24 DEG C, the intensity of illumination is controlled to 2,200lx to 2,800lx, and the duration of solar radiation is 14 to 15 hours, then, taking out the rhubarb young seedlings, placing the rhubarb young seedlings at a shady and cool ventilating place, covering the rhubarb young seedlings with clean cotton fabrics, sprinkling sterile water at any time, and carrying out seedling airing for 2 to 3 hours; inoculating a seedling rejuvenating culture medium with the young seedlings at an inoculum density of 80 plants per square meter to 100 plants per square meter under the standard that the roots of the young seedlings are completely submerged into the culture medium, then, sealing the culture medium, controlling a day temperature to 22 DEG C to 24 DEG C and a night temperature to 14 DEG C to 15 DEG C, and controlling relative humidity of air to 50% to 70%, duration of solarradiation to 15 to 17 hours and intensity of illumination to 2,200lx to 2,800lx; and spraying an aqueous solution of salicylic acid, polysaccharides and FeSO4 or an aqueous solution of jasmonic acid,the polysaccharides, the FeSO4 and calcium superphosphate to unsealed stems and leaves at intervals of 2 days, cleaning leaf surfaces with sterile water before spraying at a time, and carrying out seedling rejuvenating culture for 12 to 15 days. According to the method, the rhubarb young seedlings cultured by adopting the method are good in growth vigor, robust in rhizomes and tenacious in vitality and are free of plant diseases and insect pests after transplanting.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for cultivating rhubarb seedlings for rejuvenation. Background technique [0002] Rhubarb, also known as General, is a perennial tall herbaceous plant of the Polygonaceae Rhubarb genus, mostly grown on mountain forest edges or grass slopes. It likes a cool climate and is afraid of high temperature. It is suitable for growing in a temperature environment of 5-25°C. If the temperature exceeds 28°C, it will grow slowly, and it will die if the temperature continues. Rhubarb grows well in mountainous areas above 1500m above sea level. It grows quickly from April to June, slows down or even stops growing in July, and resumes rapid growth after August-September. The plant will not bloom until the third year. The temperature for seed germination is 10-13 ℃, the most suitable temperature is 15-20℃, as long as the soil is moist and the temperature is ...

Claims

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Application Information

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IPC IPC(8): A01G31/00C05G3/00C05G5/20
CPCA01G31/00C05G3/00C05B7/00
Inventor 韩智杰王义存杨建辉张冬梅
Owner 甘肃步云农牧科技有限责任公司
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