A kind of biological fungicide for crude oil transportation pipeline and its application
A technology of biological fungicides and transportation pipelines, applied in the field of environmental engineering, can solve the problems of difficult coating, high cost, and non-environmental protection, and achieve the effects of inhibiting microbial corrosion, avoiding drug resistance, and improving corrosion resistance
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Embodiment 1
[0033] The preparation of embodiment 1 biocides
[0034] Take the alkanophagous bacteria stored in 2216E liquid medium containing 20% glycerol at -80°C, thaw at 25°C, inoculate 1000 μl in 2216E liquid medium, and shake at 37°C at 120r / min Cultivate for 24 hours, obtain the bacterial liquid, and dilute the bacterial liquid to 1×10 8 CFU / ml, the biocide is obtained.
Embodiment 2
[0035] The inhibitory effect of embodiment 2 biological bactericides to corrosion bacteria
[0036] Get the biological fungicide prepared by 0.001kg embodiment 1 and dilute 500-1000 times and join in the postgate substratum, then add sulfate-reducing bacteria (SRB), the addition of sulfate-reducing bacteria (SRB) and the addition of fungicide Same, its ratio is 1:1. In this embodiment, an experimental group and a control group are set up. The experimental group contains both fungicides (Alkanophagous boku) and SRB, and the control group contains only SRB.
Embodiment 3
[0037] Two kinds of bacteria relative content and pH measurement in the embodiment 3 system
[0038] Take 50ml samples of the experimental group and control group in Example 2 on 1 day, 3 days, 5 days, 7 days, 9 days, 11 days, and 14 days respectively, and send them to Meiji Biological Company for biodiversity detection, and measure The pH values of the two groups.
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