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RNA purification liquid as well as preparation method and application thereof

A purification method and body fluid sample technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of poor purification effect of trace RNA, and achieve the effect of simple and rapid purification method, good purification effect and low cost

Pending Publication Date: 2020-05-19
上海力拜生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a RNA purification solution and its preparation method and application, which are used to solve the problem of poor purification effect of trace RNA in the prior art

Method used

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  • RNA purification liquid as well as preparation method and application thereof
  • RNA purification liquid as well as preparation method and application thereof
  • RNA purification liquid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0054] The preparation of embodiment 1 RNA purification solution

[0055] Prepare the RNA purification solution according to the following formula, wherein, based on the total amount of the RNA purification solution, the percentage of each component is:

[0056]

[0057]

Embodiment 2

[0058] The preparation of embodiment 2 RNA purification solution

[0059] Prepare the RNA purification solution according to the following formula, wherein, based on the total amount of the RNA purification solution, the content of each component is:

[0060]

Embodiment 3

[0061] The preparation of embodiment 3 RNA purification solution

[0062] Prepare the RNA purification solution according to the following formula, wherein, based on the total amount of the RNA purification solution, the percentage of each component is:

[0063]

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Abstract

The invention provides an RNA purification liquid as well as a preparation method and application thereof. In the RNA purification liquid, the purification liquid contains at least the following components based on the total amount of the purification liquid: sodium dodecyl sulfate with a content of 5 g / 100ml-50 g / 100ml, guanidine thiocyanate with a content of 10 g / 100ml-30 g / 100ml, sodium citratewith a content of 0.01 g / 100ml-2 g / 100ml, N-alkanoylsarcosine with a content of 0.01 g / 100ml-2 g / 100ml, mercaptoethanol with a volume percentage of 0.01%-2%, polyethylene glycol octylphenyl ether with a volume percentage of 0.01%-2%, ethylenediaminetetraacetic acid disodium salt with a content of 0.01 g / 100ml-0.5 g / 100ml, and solvent water. The RNA purification liquid provided by the invention has low costs and a good purification effect, and a purification method is simple and rapid, can be completed within 40 minutes, and can be carried out under room-temperature conditions.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to an RNA purification liquid and its preparation method and application. Background technique [0002] Messenger RNA (mRNA) is a template for cells to synthesize proteins. All kinds of cells have their specific mRNA expression, and the level of expression reflects the metabolic state of cells under physiological and pathological conditions. Unlike DNA, RNA, especially mRNA, is easily degraded during purification and extraction, which brings great trouble to research work. Currently, in the field of molecular biology research, there are usually two methods for purifying and extracting RNA: phenol (Trizol) / chloroform extraction method and membrane adsorption purification method. Both of these methods are suitable for samples with high RNA content, but for samples with small RNA content, both methods have shortcomings. The phenol (Trizol) / chloroform extraction method take...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 朱民雷芙蓉
Owner 上海力拜生物科技有限公司
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