Application of alkane compound pristane as immunopotentiator in preparation of drugs for preventing and treating solid tumors

Abstract

The invention discloses an application of alkane compound pristane as an immunopotentiator in preparation of drugs for preventing and treating solid tumors. The invention finds for the first time thatthe natural compound alkane compound pristane can be used for preparing a systemic immunopotentiator, and finds the application of the systemic immunopotentiator in preparation of the drugs for preventing and treating solid tumors. The invention proves that the low-dose pristane can be used for effectively preventing breast tumors, inhibiting lung metastasis of the breast tumors, improving prognosis survival, effectively inhibiting liver metastasis and lung metastasis of melanoma, and increasing depilation-free survival rate. Research results show that enhancing the overall immunity is an effective strategy for preventing and treating solid tumors. In addition, the invention finds and proves that the alkane compound pristane can become a novel potential medicine for preventing and treating solid tumors.

Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to the application of alkane compound pristane as an immune supplement enhancer in the preparation of drugs for the prevention and treatment of solid tumors. Background technique

[0002] Cancer is a major disease that threatens human life and health. According to the statistics of the World Health Organization (WHO), in 2018, there were 18.1 million new cancer patients worldwide, and about 9.6 million people died of cancer in 2018. It is the most important goal of current medical research to carry out cancer-related basic and translational research, so as to develop preventive and therapeutic drugs for cancer and promote cancer prevention and treatment.

[0003] Through basic and translational research in recent decades, mainly focusing on regulating adaptive immunity by targeting cell surface antigens / receptors or blocking immune checkpoints, related tumor immunotherapy has developed r...

Examples

Embodiment 1

[0052] cell experiment

[0053] 1. Cell culture

[0054] (1) Configure 1640 complete medium: add 50 mL of standard fetal bovine serum and 5.5 mL of penicillin-streptomycin to every 500 mL of RPMI 1640;

[0055] (2) Take out the 4T1 / B16F10 cells from the liquid nitrogen tank, put them into a 37°C water bath to dissolve the cells rapidly;

[0056] (3) Transfer the melted cells to a 1.5mL centrifuge tube, centrifuge at 900xg for 5min, discard the supernatant and resuspend the cells with 1mL1640 complete medium;

[0057] (4) Transfer the resuspended cells to a 10cm cell culture dish, add 1640 complete medium to 10mL and shake gently, at 37°C, 5% CO 2 Culture in a sterile incubator until the cell density reaches 90%.

[0058] 2. Cell count

[0059] (1) When the cell density reaches 90% and is in good condition, discard the old medium, add 1mL of 1*PBS buffer, wash the cells and discard the PBS;

[0060] (2) Add 1 mL of trypsin, shake the culture dish lightly to ensure that the...

Embodiment 2

[0063] Animal experiment

[0064] Mice Handling

[0065] (1) Take 12 7-week-old FVB / N-Tg(MMTV-PyMT) 634Mul transgenic mice, divide them into two groups, inject 100uL normal saline and 100uL pristane subcutaneously, raise them in SPF environment, and record the weight of the mice every week , tumor formation, tumor number and volume, the mice were sacrificed after 20 weeks of age to count lung metastases, and the specific process of counting lung metastases was as follows: take 20 weeks of age FVB / N-Tg (MMTV-PyMT) 634Mul transgenic mice, each Mice were anesthetized by injecting 500uL of pentobarbital sodium. After anesthesia, the mice were sacrificed by cervical dislocation. The mice were dissected to obtain lung tissue; the lung tissue was immersed in Bouin's fixative solution for staining for 5 minutes, and after staining, the fixative solution was discarded and an appropriate amount of 1* PBS buffer solution, pH 7.4 (submerged), shaken, after washing away the staining solut...

technical field

[0001] The invention relates to the field of biomedicine, in particular to the application of alkane compound pristane as an immune supplement enhancer in the preparation of drugs for the prevention and treatment of solid tumors. Background technique

[0002] Cancer is a major disease that threatens human life and health. According to the statistics of the World Health Organization (WHO), in 2018, there were 18.1 million new cancer patients worldwide, and about 9.6 million people died of cancer in 2018. It is the most important goal of current medical research to carry out cancer-related basic and translational research, so as to develop preventive and therapeutic drugs for cancer and promote cancer prevention and treatment.

[0003] Through basic and translational research in recent decades, mainly focusing on regulating adaptive immunity by targeting cell surface antigens / receptors or blocking immune checkpoints, related tumor immunotherapy has developed r...

Examples

Embodiment 1

[0052] cell experiment

[0053] 1. Cell culture

[0054] (1) Configure 1640 complete medium: add 50 mL of standard fetal bovine serum and 5.5 mL of penicillin-streptomycin to every 500 mL of RPMI 1640;

[0055] (2) Take out the 4T1 / B16F10 cells from the liquid nitrogen tank, put them into a 37°C water bath to dissolve the cells rapidly;

[0056] (3) Transfer the melted cells to a 1.5mL centrifuge tube, centrifuge at 900xg for 5min, discard the supernatant and resuspend the cells with 1mL1640 complete medium;

[0057] (4) Transfer the resuspended cells to a 10cm cell culture dish, add 1640 complete medium to 10mL and shake gently, at 37°C, 5% CO 2 Culture in a sterile incubator until the cell density reaches 90%.

[0058] 2. Cell count

[0059] (1) When the cell density reaches 90% and is in good condition, discard the old medium, add 1mL of 1*PBS buffer, wash the cells and discard the PBS;

[0060] (2) Add 1 mL of trypsin, shake the culture dish lightly to ensure that the...

Embodiment 2

[0063] Animal experiment

[0064] Mice Handling

[0065] (1) Take 12 7-week-old FVB / N-Tg(MMTV-PyMT) 634Mul transgenic mice, divide them into two groups, inject 100uL normal saline and 100uL pristane subcutaneously, raise them in SPF environment, and record the weight of the mice every week , tumor formation, tumor number and volume, the mice were sacrificed after 20 weeks of age to count lung metastases, and the specific process of counting lung metastases was as follows: take 20 weeks of age FVB / N-Tg (MMTV-PyMT) 634Mul transgenic mice, each Mice were anesthetized by injecting 500uL of pentobarbital sodium. After anesthesia, the mice were sacrificed by cervical dislocation. The mice were dissected to obtain lung tissue; the lung tissue was immersed in Bouin's fixative solution for staining for 5 minutes, and after staining, the fixative solution was discarded and an appropriate amount of 1* PBS buffer solution, pH 7.4 (submerged), shaken, after washing away the staining solut...

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