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Application of natural attapulgite as natural nano mineral enzyme

A kind of attapulgite, natural technology, applied in the field of enzyme catalysis, can solve the problems of undiscovered applications, etc., and achieve the effect of easy acquisition, high temperature resistance pH range, and low cost

Active Publication Date: 2020-06-09
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no related applications in other fields have been found so far

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  • Application of natural attapulgite as natural nano mineral enzyme
  • Application of natural attapulgite as natural nano mineral enzyme
  • Application of natural attapulgite as natural nano mineral enzyme

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preparation example Construction

[0034] In the present invention, the preparation method of the natural attapulgite dispersion includes: grinding the natural attapulgite, mixing the obtained powder with water, and performing ultrasonic treatment to obtain a natural attapulgite dispersion. In the present invention, the time of the grinding treatment is preferably 20 to 180 min, more preferably 50 to 150 min, and the rotating speed is preferably 20 to 100 r / min, more preferably 50 to 80 r / min; the time of the ultrasonic treatment is preferably 20-180 min, more preferably 50-150 min, and the power is preferably 40 kHz. The invention can ensure that the natural attapulgite has excellent enzyme activity by controlling the conditions of grinding treatment and ultrasonic treatment.

[0035] In the present invention, the natural attapulgite has peroxidase-like activity, catalase-like activity or superoxide dismutase-like activity.

[0036] In the present invention, when the natural attapulgite has peroxidase-like ac...

Embodiment 1

[0055] Natural attapulgite has peroxidase-like catalytic activity (qualitative test):

[0056] Reagents: 3,3,5,5-tetramethylbenzidine (TMB) was purchased from Sigma-Aldrich Inc. (USA), 3,3'-diaminobenzidine tetrahydrochloride (DAB) and o-phenylenediamine (OPD) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0057] Method: 50 μL TMB solution (10 mg / mL in DMSO) was placed in 0.9 mL 0.2M PB buffer (pH=3.6), and placed in a transparent vial, and 100 μL H 2 o 2 (30% (wt / vol)) and 50 μL of natural attapulgite dispersion (1 mg / mL), then replace TMB with DAB or OPD, respectively, and repeat the above steps.

[0058] Results: After five minutes, it can be observed that image 3 The color change of the indicated reagents, which proves that natural attapulgite has peroxidase-like activity, which can catalyze different substrates catalyzed by existing peroxidases (such as horseradish peroxidase) to develop color.

Embodiment 2

[0060] Natural attapulgite has peroxidase-like catalytic activity (quantitative test):

[0061] Reagent: 3,3,5,5-tetramethylbenzidine (3,3,5,5-Tetramethylbenzidine, TMB), purchased from Sigma-Aldrich Inc. (USA). 30%H 2 o 2 and sodium acetate were purchased from Beijing Chemical Reagent Company.

[0062] Method: Dispense 200 μL H 2 o 2 (30%), 100 μL TMB and 100 μL natural attapulgite dispersion (concentration of 1 mg / mL) were sequentially added to 1.8 mL of 0.1 M HAc-NaAc buffer solution (pH=3.6), wherein TMB was used as Substrate to show the color reaction of peroxidase activity, and then use Lambda 650S UV-Vis spectrophotometer to record the absorbance of color reaction (for TMB at 652nm) within a certain reaction time to express the natural attapulgite peroxidase-like activity.

[0063] result: Figure 4 For natural attapulgite and control group (water) in H 2 o 2 The class peroxidase activity control figure of catalyzing TMB under the existence situation, as can be...

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Abstract

The invention provides an application of natural attapulgite as a natural nano mineral enzyme, and belongs to the technical field of enzyme catalysis. The result of the embodiment shows that the natural attapulgite has peroxidase-like activity, catalase-like activity or superoxide dismutase-like activity, and has good biocompatibility. Compared with protease, the natural attapulgite has the advantages of being large in reserve, easy to obtain, low in cost, resistant to high temperature, wide in pH value range and the like; compared with researched and developed artificial nano-enzyme, the natural attapulgite has the advantages of being multifunctional, natural, non-toxic (from nature and free of heavy metals), good in biocompatibility, easy to obtain, free of complex processing treatment,capable of providing a growing and proliferating place for cells due to the huge surface area and the like.

Description

technical field [0001] The invention relates to the technical field of enzyme catalysis, in particular to the application of a natural attapulgite as a natural nanometer mineral enzyme. Background technique [0002] Medical technology is developing rapidly, and the demand for biomaterials with better properties is also increasing. Catalytic materials, especially enzymes, have been extensively studied because of their great role in the regulation of human physiological activities. However, the practical use of natural enzymes is limited because they are difficult and expensive to extract. Small but powerful, enzymes facilitate most reactions that occur in living systems. However, disadvantages such as high cost, poor stability, and difficult extraction have greatly limited the application of enzymes. With the increasing demand for enzymes, finding a natural non-toxic, catalytically efficient and low-cost enzyme has become the top priority of scientific research. At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J21/16B01J23/889B01J35/10B01J35/12B01J37/34A61K33/06A61K33/32A61P39/06B01J35/27
CPCB01J21/16B01J23/8892B01J37/0036B01J37/343A61K33/06A61K33/32A61P39/06B01J35/27B01J35/615C01B13/0207
Inventor 张以河梁敏敏冯峰王培霞佟望舒安琪
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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