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A kind of human hepatocellular liver cancer cell line and its application

A technology for hepatocellular carcinoma and hepatocellular carcinoma cells, applied in tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of large heterogeneity of liver cancer and lack of liver cancer drugs.

Active Publication Date: 2021-07-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, primary liver cancer has great heterogeneity, and different liver cancer cell lines have different tumor biological characteristics. Higher and higher
Moreover, there is still a lack of experimental materials in line with clinical tumor biological characteristics for liver cancer drug screening and evaluation.

Method used

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  • A kind of human hepatocellular liver cancer cell line and its application
  • A kind of human hepatocellular liver cancer cell line and its application
  • A kind of human hepatocellular liver cancer cell line and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1HCC-DJ711 cell preparation

[0022] Primary cell culture: fresh clinical liver cancer resection specimens obtained from Run Run Shaw Hospital Affiliated to Zhejiang University School of Medicine (patient, male, 59 years old, hepatocellular carcinoma of the right liver, poorly differentiated, tumor size 4×3cm, liver tissue Inner multifocal growth, with intravascular tumor thrombus formation, focal involvement of fat tissue outside the liver capsule, nodular cirrhosis) Immediately separate fresh liver cancer specimens. In a biological safety cabinet, after rinsing with fresh PBS, add a small amount of fresh 2g / L collagenase-4 solution (containing 500U / ml penicillin, 500μg / ml streptomycin sulfate, 1.25μg / ml amphotericin B) to remove blood Use sterilized scissors to chop as much as possible, transfer to a 15ml centrifuge tube after chopping, and add 10ml fresh collagenase-4 solution above, mix well, and store at 37°C, 5% CO 2 Incubate for 60 minutes. Add RPMI ...

Embodiment 2

[0025] Embodiment 2 Biology and application of human liver cancer cells

[0026] The invention adopts DMEM / F12 culture solution containing fetal bovine serum to cultivate HCC-DJ711 cells, so as to make long-term growth and stable passage in vitro. Observed under the microscope, HCC-DJ711 cells have typical epithelial cell-like morphology, growth inhibition due to loss of contact, and malignant growth. A certain amount of HCC-DJ711 cells can form tumors under the skin of nude mice, indicating that the cells have tumorigenic properties. The cell can be used for the research on the mechanism of the occurrence and development of liver cancer. At the same time, it can be used to screen and evaluate anti-liver cancer drugs, discover drugs sensitive to liver cancer, guide clinical medication, and open up new ideas for liver cancer treatment. details as follows:

[0027] Morphological observation

[0028] Place the culture flasks cultured with HCC-DJ711 cells under an inverted mic...

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Abstract

The invention discloses a human hepatocellular liver cancer cell line and its construction method and application. The liver cancer cell line is named human hepatocellular carcinoma cell line HCC‑DJ711, and was deposited in the China Center for Type Culture Collection on April 15, 2018, with the preservation number CCTCC NO: C201889. The invention extracts and separates cells from clinical liver cancer specimens, and the liver cancer cell line has good animal tumorigenic properties, and can be used as a cell model for studying the mechanism of occurrence and development of liver cancer. At the same time, it can be used for liver cancer drug screening and evaluation, and guide clinical medication.

Description

technical field [0001] The invention relates to the fields of biology and oncology, and relates to a human liver cancer cell line and its construction method and application. Background technique [0002] Liver cancer is the most common liver tumor. There are about 1 million new cases of liver cancer every year in the world. It ranks sixth in the incidence of cancer worldwide and is the third leading cause of cancer-related death. As a big country with hepatitis B, primary liver cancer is the most common tumor in my country. The incidence and mortality of liver cancer are among the highest in the world, and new cases and deaths each year account for more than 50% of the national cases. At present, the incidence of liver cancer in my country is high, the mortality rate is high, and the cure rate is low. It often progresses rapidly. Most patients have entered the middle and late stages when they are diagnosed. If intrahepatic metastasis, vascular invasion or distant metastasis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02
CPCC12N5/0693G01N33/5011G01N2500/00
Inventor 蔡秀军徐俊杰岑栋万喆梁霄梁岳龙
Owner ZHEJIANG UNIV
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