Human ovarian squamous carcinoma cell line and application thereof

A cancer cell, cell line technology, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of lack of ovarian squamous cell carcinoma cell lines, restricting the preclinical research of ovarian squamous cell carcinoma, etc. The effect of strong proliferative activity

Pending Publication Date: 2022-04-05
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the great differences in pathogenesis, molecular characteristics and drug efficacy of different pathological types of ovarian cancer, the lack of ovarian squamous cell lines has restricted the preclinical research on ovarian squamous cell carcinoma to a certain extent.

Method used

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  • Human ovarian squamous carcinoma cell line and application thereof
  • Human ovarian squamous carcinoma cell line and application thereof
  • Human ovarian squamous carcinoma cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Establishment of ZOC254 cell line

[0047] 1.1 The source of the specimen:

[0048] On August 8, 2017, a postoperative specimen was taken from a 64-year-old patient with ovarian squamous cell carcinoma in our hospital. Abdominal pain and distension, check CA125: 100.3U / ml; CEA: 54.2ng / ml; CA199: 375U / ml; check abdominal and pelvic CT: abdominal and pelvic cystic solid mass (16.5×9.2cm), sign the patient’s informed consent before operation .

[0049] 1.2 Specimen processing:

[0050] Fresh ovarian squamous cell carcinoma tissue specimens obtained by surgical resection were taken, rinsed with normal saline three times, cut off hemorrhagic necrotic tissue, soaked in PBS (containing penicillin 500 IU / mL, streptomycin 500 μg / mL) at 4°C for 10 min, and rinsed with normal saline repeatedly for a total of 3 times.

[0051] Obtaining primary cells: Soak ovarian squamous cell carcinoma tissue specimens in DMEM / F12 medium (purchased from Gibco) under sterile conditi...

Embodiment 2

[0056] Example 2: Biological characteristics and application of ZOC254 cell line

[0057] (1) Morphological observation of ZOC254 cell line

[0058] The subcultured ZOC254 cells were taken, and the growth of living cells was observed under an optical microscope (Olympus BX43 biological microscope, Japan) (100×). see results Figure 1A ~ Figure 1C .

[0059] Figure 1A ~ Figure 1C The optical morphology pictures of the P10, P20, and P30 generation cells of the ZOC254 cell line in turn. Depend on Figure 1A ~ Figure 1C It can be seen that the properties of the cells are stable after multiple passages, and the morphology of the P10, P20, and P30 generation cells is the same. A few are multinucleated, with a large ratio of nuclei to plasma.

[0060] (2) Genetic material STR analysis and homology identification of ZOC254 cell line

[0061] Short tandem repeat (short tandem repeat, STR), also known as microsatellite DNA, refers to a type of DNA sequence formed by tandem repetiti...

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Abstract

The invention discloses a human ovarian squamous carcinoma cell line and application thereof, the human ovarian squamous carcinoma cell line is named as a human ovarian squamous carcinoma cell line ZOC254 and is preserved in China Center for Type Culture Collection on November 3, 2021, and the preservation number is CCTCC NO: C202159. The human ovarian squamous carcinoma cell line is stable in character, can be passaged in vitro for a long time, has relatively strong proliferation activity, is histologically classified as squamous carcinoma, is positive in homologous recombination repair defect (HRD) and high in tumor mutation load (TMB-H), has targeted therapy related gene mutation and has tumorigenicity. The human ovarian squamous carcinoma cell line can be used as an effective cell model, a xenotransplantation animal model can be established, and a new experimental model can be provided for deeply researching occurrence, development and metastasis mechanisms, drug resistance mechanisms, drug screening, drug efficacy evaluation and the like of ovarian squamous carcinoma.

Description

technical field [0001] The invention belongs to the field of microbial animal cells, and in particular relates to a human ovarian squamous cell line and application thereof. Background technique [0002] Primary squamous cell carcinoma of the ovary is a squamous cell carcinoma that occurs in the ovary. It is a rare malignant tumor, and the incidence rate accounts for only 0.5% of ovarian cancer. Primary squamous cell carcinoma of the ovary is formed by cancerous change of the surface epithelial cells of the ovary, without precondition lesions and metastasis to other organs is excluded. The disease has a low incidence, rapid progression, and poor prognosis. The pathogenesis and etiology are still unclear, and the clinical manifestations are non-specific. Early diagnosis of the disease is difficult, and most patients complain of abdominal pain, abdominal distension, constipation, frequent urination and other late-stage tumor compression symptoms. There is no effective auxili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
Inventor 苏丹徐晨阳张平应莉莎黄旻然
Owner ZHEJIANG CANCER HOSPITAL
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