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A human liver cancer cell line overexpressing fgf19 and its application

A liver cancer cell, overexpression technology, applied in the field of cells, can solve the problems of lack, drinking water pollution, poor prognosis of liver cancer patients, etc., and achieve the effect of stable traits

Active Publication Date: 2022-04-26
SHANGHAI EAST HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Pollution of drinking water
Therefore, the overall prognosis of patients with primary liver cancer is poor, and it is urgent to seek more effective treatments.
[0006] At present, there is a lack of experimental materials close to clinical tumor biological characteristics for the mechanism of liver cancer and the development of anti-liver cancer drugs

Method used

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  • A human liver cancer cell line overexpressing fgf19 and its application
  • A human liver cancer cell line overexpressing fgf19 and its application
  • A human liver cancer cell line overexpressing fgf19 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1LI

[0046] The preparation of embodiment 1LIXC-501 cell

[0047] A. Preparation of LIXC-501 hepatocellular carcinoma human mouse model

[0048] (1) A series of fresh resection specimens of clinical liver cancer were obtained from Shanghai Dongfang Hospital. The clinical diagnosis was recurrence of liver cancer, and the pathological diagnosis was hepatocellular carcinoma, some of which were clear cell subtype.

[0049] Rinse the above specimen with fresh HBSS buffer (HBSS buffer containing 500 U / mL penicillin G, 500 μg / mL streptomycin sulfate and 1.25 μg / mL amphotericin B), cut into small pieces of 20-50 mg, and subcutaneously Immunodeficient animal SCID mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were puncture-inoculated; after tumor formation, human-derived mouse models of hepatocellular carcinoma were prepared and named respectively. Tumors continued to be passaged in vivo and cryopreserved.

[0050] (2) Through next-generation sequenci...

Embodiment 2LI

[0059] Biological characteristics and application of embodiment 2LIXC-501 cells

[0060] The invention adopts RPMI1640 culture and purifies LIXC-501 cells by a differential attachment method, so that they can grow long-term in vitro and pass stably. When the cells are passed on for more than 20 generations, the cell traits are gradually stabilized, and relevant biology, genetics, and tissue source identification are carried out until the 30th to 50th generations have the same stable traits. According to experimental observation and verification, LIXC-501 grown in vitro has a typical epithelial-like morphology, the main body is flat irregular polygon, with a round nucleus in the middle, and grows in a covering layer. The cells adhere to the wall and are closely connected with each other. shape move. Genetic studies have confirmed that the aneuploid ratio of the cells is about 50%, and the number and structure of chromosomes are seriously aberrated, which is consistent with the...

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Abstract

The invention discloses a human liver cancer cell line overexpressing FGF19 and application thereof. The human liver cancer cell overexpressing FGF19 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: C201663. The human liver cancer cells have stable properties, can be stably passed down for multiple times, have a high tumor formation rate, a short incubation period, and good uniformity, providing new experimental materials for liver cancer research that are closer to the biological characteristics of clinical tumors. At the same time, it has the ability to form tumors after being inoculated in nude mice. Compared with the passaged parental tumors, it can be used to analyze drug sensitivity in vitro and in vivo, and then establish a drug screening platform. It is an ideal cell for basic research and preclinical applications of human liver cancer. .

Description

technical field [0001] The invention belongs to the field of cells, in particular to a human liver cancer cell line overexpressing FGF19 and application thereof. Background technique [0002] Primary liver cancer is one of the common malignant tumors of the digestive tract. There are about 1 million new cases of liver cancer every year in the world. It is the sixth most common cancer in the world and the third leading cause of cancer death in the world. The incidence of primary liver cancer has obvious regional characteristics. my country is a country with a high incidence of the disease. Its incidence rate accounts for 55% of the world, and its mortality rate accounts for 45% of the world. At present, about 320,000 cases of liver cancer patients die every year. In my country, primary liver cancer ranks second in the incidence of malignant tumors. In recent years, it has gradually increased. It has the characteristics of high morbidity, high mortality and low cure rate. Ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A01K67/027A61K49/00
Inventor 郑亚新过欣来杨冰谢付波李富林凯汪宗宇周禾
Owner SHANGHAI EAST HOSPITAL
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