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Semen plantaginis active part and preparation method thereof

An active part, step two technology, applied in the field of psyllium active part and its preparation, can solve the problems of uneven antioxidant effect, restricting clinical application, low oral availability, etc.

Inactive Publication Date: 2020-07-28
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of different anti-oxidation effects of various chemical components of Semen Plantago in the prior art, low direct oral availability, which restricts clinical application, and provides an active part of Semen Plantago and its preparation method

Method used

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  • Semen plantaginis active part and preparation method thereof
  • Semen plantaginis active part and preparation method thereof
  • Semen plantaginis active part and preparation method thereof

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preparation example Construction

[0029] The preparation method of the plantago active part of the present invention comprises the following steps:

[0030] Step 1. Mix Plantaginaceae with ethanol solution with a concentration of 75vol%, break the wall and pulverize, let stand for more than 1 hour, heat and reflux at 80-82°C for more than 2 hours, filter, concentrate to a crude drug amount of 2.5-5.0g / mL, and obtain Concentrate;

[0031] Step 2: After the concentrated solution is absorbed by a macroporous adsorption resin, it is left to stand for more than 2 hours, rinsed, and then eluted with an ethanol solution with a concentration of 15vol%-70vol%, and the eluate is collected, concentrated, and freeze-dried to obtain Plantago subactive site.

[0032] In the above technical solution, in step 1, it is preferable to use a flash extractor for wall breaking and crushing, and the conditions are usually that the voltage is 100V, and the duration is more than 90s, and the wall breaking and crushing cannot be compl...

Embodiment 1

[0047] Step 1: Take 200g of Semen Plantaginis, add 2000mL of 75vol% ethanol solution, use a flash extractor to break the wall and pulverize it, then let it stand for 1h, heat and reflux for 2h, filter, and the filtrate obtained is the total extract solution of Semen Plantaginis, Then rotary steaming to the crude drug amount of 2.5g / mL to obtain a concentrated solution.

[0048]Step 2: Take 8mL of the concentrated solution, put it on the AB-8 macroporous adsorption resin with a column volume of 20mL at a speed of 1BV / h, after standing for 2h, first elute with 100mL ultrapure water for 3h, and then use 15vol% , 50vol%, 70vol% ethanol solution as the elution solvent, the elution volume is 3BV, 3BV, 3BV respectively, and the elution speed is 2BV / h, collect each eluent, then concentrate and freeze-dry to obtain Semen Plantaginis Freeze-dried powder of each active part.

[0049] The mass spectrum of Semen Plantaginis total extract solution and each active part of Semen Plantago tha...

Embodiment 2

[0051] Step 1: Take 200g of Semen Plantaginis, add 2000mL of 75vol% ethanol solution, use a flash extractor to break the wall and pulverize it, then let it stand for 1h, heat and reflux for 2h, filter, and the filtrate obtained is the total extract solution of Semen Plantaginis, Then rotary steaming to the crude drug amount 5g / mL to obtain a concentrated solution.

[0052] Step 2: Take 4mL of the concentrated solution, put it on the AB-8 macroporous adsorption resin with a column volume of 20mL at a speed of 1BV / h, after standing for 2h, first elute with 100mL ultrapure water for 3h, and then use 15vol% , 50vol%, 70vol% ethanol solution as the elution solvent, the elution volume is 3BV, 5BV, 5BV respectively, and the elution speed is 2BV / h, collect each eluent, then concentrate and freeze-dry to obtain Semen Plantaginis Freeze-dried powder of each active part.

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Abstract

The invention relates to a semen plantaginis active part and a preparation method thereof, and belongs to the technical field of extracts. The technical problems that in the prior art, chemical components of semen plantaginis are different in anti-oxidation effect, the direct oral administration availability is low, and clinical application is restricted are solved. The preparation method of the semen plantaginis active part comprises the following steps of firstly, mixing semen plantaginis with an ethanol solution, carrying out wall-breaking crushing, standing, carrying out heating reflux, and concentrating until the crude drug amount is 2.5-5.0 g / mL, so as to obtain a concentrated solution; and adsorbing the concentrated solution by using macroporous adsorption resin, washing, eluting byusing an ethanol solution with the concentration of 15vol%-70vol%, collecting the eluate, concentrating, and freeze-drying to obtain the active part of the semen plantaginis. According to the preparation method, the active part of the semen plantaginis is separated through a column chromatography technology, wherein the elution part of the ethanol solution with the concentration of 15vol%-70vol%has an anti-oxidation effect, and particularly the elution part of the ethanol solution with the concentration of 50vol% has the optimal effect.

Description

technical field [0001] The invention belongs to the technical field of extracts, and in particular relates to an active part of psyllium seed and a preparation method thereof. Background technique [0002] Antioxidant refers to anti-oxidative free radicals. Antioxidant substances are any substances that can effectively inhibit the oxidation reaction of free radicals in a low concentration. The mechanism of action can be to directly act on free radicals, or to indirectly consume substance to prevent further reaction. While the human body inevitably produces free radicals, it also naturally produces antioxidant substances that resist free radicals to counteract the oxidative attacks of free radicals on human cells. Studies have proved that the antioxidant system of the human body is a system with complete and complex functions comparable to the immune system. The stronger the body's antioxidant capacity, the healthier it is and the longer its life. [0003] Plantago is a com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/68A61P39/06
CPCA61K36/68A61K2236/333A61K2236/51A61K2236/55A61P39/06
Inventor 刘舒高红雪刘志强郑重宋凤瑞
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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