75 results about "Column chromatography technique" patented technology

The invention discloses natural perfume refining fluid suitable for cigarette fluid of electronic cigarettes and cigarette fluid and a preparation method thereof, and belongs to the technical field of cigarette fluid of electronic cigarettes. Separation and impurity removal are performed on natural perfume such as extract, tincture and absolute oil through a macroporous adsorption resin column chromatography technology, high-boiling-point components such as monosaccharide and disaccharide and macromolecular components such as protein, polysaccharide and pectin in the natural perfume are simultaneously and effectively removed according to the differences of polarities and molecular sizes of all the components in the mixture, the components unfavorable for the smoking mouthfeel of the electronic cigarettes are removed, and the problem that charring flavor is generated due to the fact that the substances are deposited on heating wires is solved; meanwhile, the solubleness of the natural perfume in propylene glycol and glycerinum is improved, therefore, the cigarette fluid of the electronic cigarettes becomes clear and bright, the emission performance of perfume is significantly improved, the sweet greasy feeling is reduced, and the sensory effect is significantly improved.

The invention relates to a production method for adsorbing a human complex from plasma by a fixed bed column chromatography technique, which comprises the following steps: (1) cryoprecipitation plasma removal: filtering by using a cellulose deep filter plate which is cleaned by an EDTA (ethylene diamine tetraacetic acid) solution and a sodium citrate solution; (2) filtering the plasma subjected to deep filtration through a 0.2 mu m filter element membrane while fixed bed loading; (3) balancing 2-5 column volumes in a fixed bed chromatographic column filled with anion exchange gel Capto DEAE by using a buffer solution A at the plasma loading flow rate of 60-120 cm/hour, washing the chromatographic column with a buffer solution B, and eluting the chromatographic column with a buffer solution C to obtain a PCC (prothrombin complex concentrate) product. When the calculation is based on coagulation factor IX, the yield of the PCC can reach 75-90%, and the specific activity can reach 5.5 IU/mg above.

The invention relates to a method for preparing high-purity stevioside rebaudioside RC, and belongs to the technical field of food additives. In the method, stevioside is used as a raw material, rebaudioside RC content in the raw material is about between 3 and 15 percent, and recrystallization technology and column chromatography technology are combined. The method comprises the following steps of: performing recrystallization by dissolving the stevioside which serves as the raw material in methanol; stirring and crystallizing at the temperature of 4 DEG C for 24 to 48 hours; recovering a solvent from crystallization mother liquor under reduced pressure to obtain a concentrate; performing silica gel column chromatography on the concentrate under the conditions that a stationary phase is silica gel with particle size of between 200 and 300 meshes, the weight of the silica gel mixed with a sample is 2.5 times that of the concentrate and the mass of pure silica gel is 20 to 50 times that of the concentrate; performing elution by using a mixed solvent system of trichloromethane and ethanol in the ratio of 2 to 1 or dichloromethane and ethanol in the ratio of 2 to 1, wherein water content in the ethanol is 2 to 10 percent; and collecting rebaudioside RC-containing main section eluent, mixing, concentrating, drying, detecting and packaging. By the method, a rebaudioside C product with purity of more than 95 percent can be obtained; and the method has a simple process and is suitable for large-scale industrial production.

The present invention relates to a method for extracting and purifying functional components of Chinese herbal medicine. The purpose is to provide a simple, safe, economical and efficient method for extracting and purifying monomeric compounds vitexin and isovitexin from pigeonpea branches and leaves. The technical scheme is: using fresh or dried branches and leaves of pigeonpea as raw materials, using homogenate extraction technology, ultrasonic assisted extraction technology, ultrasonic oscillation flocculation technology, negative pressure cavitation suspension liquid extraction technology, macroporous adsorption resin enrichment technology, ODS -C18 reverse phase medium pressure silica gel column chromatography technology combined with low temperature crystallization and recrystallization technology to obtain vitexin and isovitexin with a purity of over 90%, and the extraction rates are over 0.17% and 0.13% respectively . The raw materials used in the invention are fresh or dried pigeonpea branches and leaves which are mostly discarded in agricultural production, which neither destroys the ecological balance of plants nor affects agricultural production, and can obtain high-purity pigeonpea functional components. Moreover, the method is simple and easy to implement after extraction, preliminary impurity removal and repurification, and the loss of the target compound is small. It is suitable for industrial applications and is of great significance to industrial production.

The invention relates to the field of separation and purification of active ingredients of a natural product, in particular to a method for preparing high-purity 1-deoxynojirimycin with a combined membrane separation and column chromatography technology. According to the method, raw materials of silkworm and white mulberry is subjected to water extraction in aid of ultrasonic waves; an ultrafiltration feeding liquid is obtained through centrifugation and micro-filtration membrane treatment; and a 1-deoxynojirimycin product with purity larger than 80% is obtained through steps of secondary ultrafiltration, nanofiltration, cation exchange resin chromatography and dextrane gel chromatography. The method has the advantages of simple process, high efficiency, safety and environment protection.

The invention relates to a jerk filefish skin collagen antioxidative peptide which is characterized in that the amino acid sequence of the antioxidative peptide is Asn-Glu-Gly-Ala-Cys-Gly, and the molecular ion peak detected by UPLC-MS is [M]+531m/z. The invention further relates to a preparation method of the jerk filefish skin collagen antioxidative peptide. According to the method provided by the invention, a double-enzyme fractional hydrolysis technique is combined with an ultrafiltration and gel column chromatography technique, the whole preparation process is simple and easy to control and high in hydrolysis efficiency, and the obtained antioxidative peptide is safe and free of side effects, has relatively high antioxidant activity, and not only has a good eliminating effect to DPPH.free radicals, but also has relatively good thermal stability. The antioxidative peptide digested by the gastrointestinal digestive tract still maintains relatively high antioxidant activity. In addition, the raw materials adopted by the antioxidative peptide prepared are processing wastes of a marine low-value fish jerk filefish, so that the environmental pollution is effectively avoided and the utilization ratio of resources is improved.

The invention relates to a method for extracting effective ingredient as Alpha-glucosidase inhibitor, which belongs to the fields of Chinese herbal medicine preparation and chemical technology. The method comprises the following steps of: performing liquid extraction with organic solvent to remove ingredients with low Alpha-glucosidase inhibitory activity, and extracting effective ingredients with high Alpha-glucosidase inhibitory activity by using macroporous adsorptive resin and Sephadex LH-20 column chromatography technique. The yield of the inhibitor is 4%. The inventive method has the advantages of simple process, easy operation, high product purity, no harmful substances in product, and no environmental pollution. The obtained plant extract in the invention can be used for developing hypoglycemic drugs and further researching physical structure thereof.

The invention discloses a method for synthesizing (+/-)-9-O-demethyl-alpha-dihydrotetrabenazine, which comprises the following steps of: adding sodium hydride and hexamethylphosphoramide into dimethylbenzene, heating to the temperature of between 90 and 100 DEG C, dripping N-methylaniline, and stirring for 15 to 20 minutes; dripping dimethylbenzene suspension of (+/-)-alpha-dihydrotetrabenazine into the mixed solution, after dripping, continuously stirring the reaction suspension at the temperature of between 90 and 100 DEG C for 48 to 50 hours; slowly adding aqueous solution of sodium hydroxide into the reaction suspension, separating out an aqueous phase, neutralizing the aqueous phase by using aqueous solution of hydrochloric acid until the pH value is 7 to 9, extracting by using diethyl ether, concentrating an organic phase under reduced pressure to obtain a light brown solid; and purifying the light brown solid by using column chromatography technology to obtain the target product. By optimizing the reaction conditions of the prior art and improving posttreatment and purification technology, the method has the advantages of low preparation cost, high repeatability and high yield.

The invention belongs to the technical field of plant extraction and analysis, and especially relates to a jujube root extract, an extraction and separation method and an application thereof. The jujube root extract includes the following 15 components: ursolic acid, ceanothenic acid, eugenol-beta-D-glucoside, dihydrochalcone-4'-beta-D-glucopyranoside, oleanolic acid, 3-oxooleanolic acid, catechin, betulinic acid, jujube naphthoquinone, spineless common jujube benzyl II, beta-sitosterol, stigmasterol, daucosterol, p-hydroxybenzoic acid, and quercetin. The method utilizes ethanol extraction andethyl acetate extraction, and combines the use of silica gel, CHP-20P, macroporous resin, Sephadex LH-20 and other column chromatography techniques to separate the jujube root extract, and can be applied to researches of drugs for delaying Alzheimer's disease and treating diabetes.

The present invention discloses a new kind of ceramide compound Altermides, which is extracted and separated from marine fungus Alternalia sp. fermenting liquid and has cell apoptosis activity. By means of the combined solvent extraction and column chromatography technology, one series of ceramide compounds is obtained from the fermenting liquid. The compounds have wide material source and is expected to become new anticancer medicine.

The invention belongs to the technical field of tobacco flavors and particularly discloses a method for preparing a sweetening and moistening tobacco flavor from a Dangshan crisp pear as an extract by adopting an enzymolysis approach and a column chromatography technology. Through the operations of pulping, enzymolysis, column chromatography separation and eluting, vacuum concentration and the like, on the one hand, the juice yield of the Dangshan crisp pear is improved, a natural tobacco extract is reserved to the maximal extent, the aroma quality, the sweetness, liquid-engendering feeling and the taste softness are improved; and on the other hand, macromolecular substances affecting the sensory quality of the smoke are removed to obtain the high-quality extract. The crisp pear tobacco flavor prepared by the method is natural in aroma and distinct in fragrance characteristic, and is capable of playing a role in harmonizing the smoke, giving smoke sweetness, relieving throat irritation and improving the taste after being added to a cigarette at a proper ratio.

The invention provides a separation preparation method of sotolon and an application to cigarettes. Fenugreek seeds taken as the raw material are subjected to baking, extraction and concentration, a fenugreek tincture or extract is obtained, an organic solvent is taken as an eluent system, and the sotolon is obtained through silica-gel column chromatography separation. GC-MS detection indicates that GC purity of the sotolon is higher than 99%. The method is simple to operate, low in equipment requirement and shorter in consumed time.

The invention relates to dendrobium loddigesii rolfe Loddigesiinols G-K as well as a preparation method and an application thereof in preparation of hypoglycemic drugs. The Loddigesiinols G-K are prepared by the steps as follows: (1), an extracting solution obtained after dendrobium loddigesii rolfe is extracted by methyl alcohol is subjected to pressure-reduction concentration so as to obtain an extract; (2), the extract is sequentially extracted by petroleum ether, ethyl acetate and n-butyl alcohol; and (3), an ethyl acetate extract is repeatedly purified with a Sephadex LH-20 gel column chromatography technology and separated with an HPLC (high performance liquid chromatography) technology, so that five active Loddigesiinols G-K are obtained. Experiments show that dendrobium loddigesii rolfe Loddigesiinols G-K can be used as a hypoglycemic drug, a hyperglycemic health care product and a drug and health care product for preventing and curing cardiovascular disease.

The invention relates to filefish skin collagen peptide having ACE restraining activity. The amino acid sequence of the collagen peptide is Gly-Val-Gla, and through UPLC-MS detection, the molecular ion peak is [M]+329m/z. The invention further relates to a preparation method of the collagen peptide. The preparation method comprises combined usage of mixing and hydrolysis through double enzymes, ultrafiltration and a gel column chromotography technique, the whole preparation process is simple and easy to control, the enzymolysis efficiency is high, and the obtained collagen peptide is safe andfree from side effects, has higher ACE restraining activity and also has better heat stability; besides, after being digested through digestive tracts of stomach and intestines, the collagen peptide still maintains higher ACE restraining activity; and in addition, processed waste namely fishskin is used as a raw material for preparing the collagen peptide, so that environmental pollution is effectively avoided, and the utilization rate of the resources is increased. The filefish skin collagen peptide has higher practical value and commercial value.

The invention discloses a method for purifying bromelin by a nano-titanium dioxide porous ceramic column chromatography, belonging to the technical field of enzyme separation and purification, mainly solving the problems of bromelin purification and enzymatic activity preservation. The technical scheme comprises the following steps of: manufacturing a porous ceramic column chromatography stuffing by a utilizing ceramic sintering technology; purifying the bromelin by adopting a column chromatography technology; and maximally preserving enzymatic activity through ultrafiltration and freeze-drying to obtain a finished product of the bromelin with the amount of activity units reaching 6 million. The invention is a high-efficiency and safe method for preparing the high-activity bromelin, and the product can be used as food or medicine raw material to be widely applied to the fields of food, biomedical industry and the like.

Belonging to the technical field of chemical industry, the invention in particular relates to a separation and extraction method for gentiopicroside in Picrorhiza scrophulariiflora Pennell. The invention specifically employs extraction technique, macroporous resin column chromatography and silica gel column chromatography technique to elute a lot of gentiopicroside individually from a silica gel chromatographic column, thus increasing the extraction amount of gentiopicroside, and the finally obtained gentiopicroside has purity up to 98.7-99.3%, thus greatly improving the purity of gentiopicroside. The method provided by the invention has the advantages of wide application range, convenience and rapidity, low cost, simple operation and large separation amount, thereby being suitable for large-scale popularization and application.

The invention discloses jasmine essence for a blasting bead for a cigarette and a preparation method and application of the jasmine essence. The jasmine essence is prepared from, by mass, 10-20% of benzyl acetate, 2-10% of jasmonic acid methyl ester, 10-20% of Jasmine lactone, 3-6% of nerolidol, 15-25% of linalool, 10-20% of jasmine and 10-15% of benzyl alcohol. The cigarette with jasmine flower fragrance is prepared, raw materials required for preparing the jasmine essence are separated from extract of natural jasmine products and obtained, and the defect that synthetic essence only has the shape same with a natural perfume material and no essence of the natural perfume material is overcome. Through separation of a column chromatography technology, the mixed odor influence of jasmine extract and macromolecule substances of pectin, protein, fiber and the like in the essence on the sensory quality of the cigarette is reduced. The prepared blasting bead can reduce stimulation of the cigarette, mixed odor of the cigarette is reduced, the comfort degree of the cigarette is improved, and the cigarette has the jasmine fragrance characteristic.

The invention relates to a semen plantaginis active part and a preparation method thereof, and belongs to the technical field of extracts. The technical problems that in the prior art, chemical components of semen plantaginis are different in anti-oxidation effect, the direct oral administration availability is low, and clinical application is restricted are solved. The preparation method of the semen plantaginis active part comprises the following steps of firstly, mixing semen plantaginis with an ethanol solution, carrying out wall-breaking crushing, standing, carrying out heating reflux, and concentrating until the crude drug amount is 2.5-5.0 g/mL, so as to obtain a concentrated solution; and adsorbing the concentrated solution by using macroporous adsorption resin, washing, eluting byusing an ethanol solution with the concentration of 15vol%-70vol%, collecting the eluate, concentrating, and freeze-drying to obtain the active part of the semen plantaginis. According to the preparation method, the active part of the semen plantaginis is separated through a column chromatography technology, wherein the elution part of the ethanol solution with the concentration of 15vol%-70vol%has an anti-oxidation effect, and particularly the elution part of the ethanol solution with the concentration of 50vol% has the optimal effect.

The invention discloses a method for preparing India quassin compounds, which comprises the following steps of: using India quassia as raw materials, separating substances to be extracted out of the raw materials through tissue crushing, cellulase enzymolysis and ultrasonic extraction, conducting multi-stage countercurrent extraction by using n-butanol and water mixed solution and conducting separation through magnesia column chromatography to extract India quassin substances. The method has the characteristics of high extraction rate, simple operation process, low energy consumption, low cost and the like.

The invention relates to a preparation method of imprinted polymers of core-shell type molecules, products of the imprinted polymers of the core-shell type molecules, and the application of the products. The preparation method comprises the following steps: synthesizing a seed emulsion by adopting a monomeric compound shown as a formula II and a monomeric compound shown as a formula III; using the seed emulsion as a core, adopting the monomeric compound shown as the formula I and the monomeric compound shown as the formula II to carry out random copolymerization with the seed emulsion so as to obtain the imprinted polymers of the core-shell type molecules. According to the preparation method of the imprinted polymers of the core-shell type molecules, the products of the imprinted polymers of the core-shell type molecules, and the application of the products, which are disclosed by the invention, can be used for extracting lincomycin A with higher purity from lincomycin and analogues of the lincomycin; under the water-phase condition, or in a water solution, the lincomycin A, the lincomycin B and other analogous ingredient components can be separated, so that the lincomycin A with higher purity is obtained; a better separation result can be obtained when the preparation method is combined with the columns chromatography.

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting total alkaloids from wild menispermum dauricum in great khingan, in particular to a novel method for separating and purifying total alkaloids by using biological enzymolysis and ultrahigh pressure extraction, and combining with column chromatography. The method has the advantages that 1, a biological enzymolysis technique is adopted, so that effective components of alkaloids in cells are dissolved out fully; 2, an ultrahigh pressure extraction technique is adopted, so that the extraction time can be shortened; the energy consumption can be reduced; impurity components can be prevented from being dissolved out; the yield of the effective components is increased; the purity is high; and 3, with the adoption of a column chromatography technique for adsorption and separation, extracted total alkaloids are further purified. The method adopts the biological enzymolysis method, so that the effective components are dissolved out fully; then the ultrahigh pressure extraction and the column chromatography are adopted for separation and purification; and the purity of obtained total alkaloids is increased to 93.6%.

The invention relates to a marine source antifreeze peptide preparation, the antifreeze peptide preparation contains polypeptide His-Val-Arg-Gly-Ala-Phe-Ser-Glu-Glu, the polypeptide accounts for 60-70% of the total content of the antifreeze peptide preparation, the molecular ion peak of the antifreeze peptide preparation is [M] + 1052 m/z through UPLC-MS detection, and the antifreeze peptide preparation can play a good low-temperature protection role on escherichia coli and catalase. The invention also relates to a preparation method of the marine-source antifreeze peptide preparation, the method combines a protease enzymolysis technology and a gel column chromatography technology, the operation is simple, the cost is low, the enzymolysis process efficiency is high, and the antifreeze peptide preparation obtained by separation and purification has high antifreeze activity; the adopted raw material is the squid skin of a processing byproduct, so that the utilization degree of resources can be effectively improved, and the environmental pollution is reduced.

The invention discloses a method for extracting ceramide from malus micromalus rice bran, and belongs to the technical field of separation and purification. The method comprises the following steps: performing two-enzyme enzymolysis on rice bran through sphingomyelinase and cellulase, filtering, soaking with an ethanol solution, performing ultrasonic heat extraction, extracting with petroleum ether to obtain a ceramide extract, and performing silica gel column chromatography and SPE (Solid Phase Extraction) small column on the ceramide extract to obtain ceramide. According to the method, a double-enzyme composite enzymolysis method is combined with ultrasonic heat extraction and SPE column chromatography technologies, so that efficient extraction and purification of ceramide in the malus micromalus rice bran are realized.