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76 results about "Column chromatography technique" patented technology

Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture. The mixture to be analyzed by column chromatrography is placed inside the top of the column.

Natural perfume refining fluid suitable for cigarette fluid of electronic cigarettes and cigarette fluid and preparation method thereof

ActiveCN105029681ARemove ingredients that are unfavorable to smoking tasteEfficient removalTobacco treatmentSolubilityLiquid smoke
The invention discloses natural perfume refining fluid suitable for cigarette fluid of electronic cigarettes and cigarette fluid and a preparation method thereof, and belongs to the technical field of cigarette fluid of electronic cigarettes. Separation and impurity removal are performed on natural perfume such as extract, tincture and absolute oil through a macroporous adsorption resin column chromatography technology, high-boiling-point components such as monosaccharide and disaccharide and macromolecular components such as protein, polysaccharide and pectin in the natural perfume are simultaneously and effectively removed according to the differences of polarities and molecular sizes of all the components in the mixture, the components unfavorable for the smoking mouthfeel of the electronic cigarettes are removed, and the problem that charring flavor is generated due to the fact that the substances are deposited on heating wires is solved; meanwhile, the solubleness of the natural perfume in propylene glycol and glycerinum is improved, therefore, the cigarette fluid of the electronic cigarettes becomes clear and bright, the emission performance of perfume is significantly improved, the sweet greasy feeling is reduced, and the sensory effect is significantly improved.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Method for adsorbing human prothrombin complex from plasma

ActiveCN104109202AHigh yieldHigh activityPeptide preparation methodsPeptidasesProthrombin complex concentrateCellulose
The invention relates to a production method for adsorbing a human complex from plasma by a fixed bed column chromatography technique, which comprises the following steps: (1) cryoprecipitation plasma removal: filtering by using a cellulose deep filter plate which is cleaned by an EDTA (ethylene diamine tetraacetic acid) solution and a sodium citrate solution; (2) filtering the plasma subjected to deep filtration through a 0.2 mu m filter element membrane while fixed bed loading; (3) balancing 2-5 column volumes in a fixed bed chromatographic column filled with anion exchange gel Capto DEAE by using a buffer solution A at the plasma loading flow rate of 60-120 cm / hour, washing the chromatographic column with a buffer solution B, and eluting the chromatographic column with a buffer solution C to obtain a PCC (prothrombin complex concentrate) product. When the calculation is based on coagulation factor IX, the yield of the PCC can reach 75-90%, and the specific activity can reach 5.5 IU / mg above.
Owner:SHANDONG TAIBANG BIOLOGICAL PROD CO LTD

Method for preparing rebaudioside C

The invention relates to a method for preparing high-purity stevioside rebaudioside RC, and belongs to the technical field of food additives. In the method, stevioside is used as a raw material, rebaudioside RC content in the raw material is about between 3 and 15 percent, and recrystallization technology and column chromatography technology are combined. The method comprises the following steps of: performing recrystallization by dissolving the stevioside which serves as the raw material in methanol; stirring and crystallizing at the temperature of 4 DEG C for 24 to 48 hours; recovering a solvent from crystallization mother liquor under reduced pressure to obtain a concentrate; performing silica gel column chromatography on the concentrate under the conditions that a stationary phase is silica gel with particle size of between 200 and 300 meshes, the weight of the silica gel mixed with a sample is 2.5 times that of the concentrate and the mass of pure silica gel is 20 to 50 times that of the concentrate; performing elution by using a mixed solvent system of trichloromethane and ethanol in the ratio of 2 to 1 or dichloromethane and ethanol in the ratio of 2 to 1, wherein water content in the ethanol is 2 to 10 percent; and collecting rebaudioside RC-containing main section eluent, mixing, concentrating, drying, detecting and packaging. By the method, a rebaudioside C product with purity of more than 95 percent can be obtained; and the method has a simple process and is suitable for large-scale industrial production.
Owner:SHENYANG TIANFENG BIOLOGICAL PHARMA

Method for extracting and purifying vitexin and isoviextin from cajanus cajan branches and leaves

The present invention relates to a method for extracting and purifying functional components of Chinese herbal medicine. The purpose is to provide a simple, safe, economical and efficient method for extracting and purifying monomeric compounds vitexin and isovitexin from pigeonpea branches and leaves. The technical scheme is: using fresh or dried branches and leaves of pigeonpea as raw materials, using homogenate extraction technology, ultrasonic assisted extraction technology, ultrasonic oscillation flocculation technology, negative pressure cavitation suspension liquid extraction technology, macroporous adsorption resin enrichment technology, ODS -C18 reverse phase medium pressure silica gel column chromatography technology combined with low temperature crystallization and recrystallization technology to obtain vitexin and isovitexin with a purity of over 90%, and the extraction rates are over 0.17% and 0.13% respectively . The raw materials used in the invention are fresh or dried pigeonpea branches and leaves which are mostly discarded in agricultural production, which neither destroys the ecological balance of plants nor affects agricultural production, and can obtain high-purity pigeonpea functional components. Moreover, the method is simple and easy to implement after extraction, preliminary impurity removal and repurification, and the loss of the target compound is small. It is suitable for industrial applications and is of great significance to industrial production.
Owner:NORTHEAST FORESTRY UNIVERSITY +1

Method for preparing high-purity 1-deoxynojirimycin with combined membrane separation and column chromatography technology

InactiveCN103274992AReasonable separation processEfficient separationOrganic chemistryFiltration membraneUltrafiltration
The invention relates to the field of separation and purification of active ingredients of a natural product, in particular to a method for preparing high-purity 1-deoxynojirimycin with a combined membrane separation and column chromatography technology. According to the method, raw materials of silkworm and white mulberry is subjected to water extraction in aid of ultrasonic waves; an ultrafiltration feeding liquid is obtained through centrifugation and micro-filtration membrane treatment; and a 1-deoxynojirimycin product with purity larger than 80% is obtained through steps of secondary ultrafiltration, nanofiltration, cation exchange resin chromatography and dextrane gel chromatography. The method has the advantages of simple process, high efficiency, safety and environment protection.
Owner:YANGZHOU UNIV

Jerk filefish skin collagen antioxidative peptide and preparation method thereof

The invention relates to a jerk filefish skin collagen antioxidative peptide which is characterized in that the amino acid sequence of the antioxidative peptide is Asn-Glu-Gly-Ala-Cys-Gly, and the molecular ion peak detected by UPLC-MS is [M]+531m / z. The invention further relates to a preparation method of the jerk filefish skin collagen antioxidative peptide. According to the method provided by the invention, a double-enzyme fractional hydrolysis technique is combined with an ultrafiltration and gel column chromatography technique, the whole preparation process is simple and easy to control and high in hydrolysis efficiency, and the obtained antioxidative peptide is safe and free of side effects, has relatively high antioxidant activity, and not only has a good eliminating effect to DPPH.free radicals, but also has relatively good thermal stability. The antioxidative peptide digested by the gastrointestinal digestive tract still maintains relatively high antioxidant activity. In addition, the raw materials adopted by the antioxidative peptide prepared are processing wastes of a marine low-value fish jerk filefish, so that the environmental pollution is effectively avoided and the utilization ratio of resources is improved.
Owner:ZHEJIANG WANLI UNIV

Method for preparing compound of vegetative phosphatidylinositol in high purity

A process for preparing the high-purity (more than 96%) vegetative phosphatidylinositol (PI) from soybean includes such steps as extracting in solvent, separating, and purifying by macroreticular resin chromatography. Said PI has high activity of selectively killing more kinds of human cancer cells and preventing and treating senile dementia.
Owner:商宗一

Single sialic acid tetrahexose ganglioside preparation method

The invention relates to a method for preparing mono sialic acid hexose ganglioside sodium agent, comprising removing integument and blood vessel from the fresh or frozen pig brain or cattle brain, pushing for dewatering with cold acetone for several times, filtering with organic membrane, pouring out the acetone, and treating into acetone powder, packing, and conserving; extracting clarified solution from acetone powder, filtering with ultra-filter membrane and getting ultra-filtrate, extracting with tobacco extract, sodium filtering with sodium filter membrane to remove small molecule, abandoning the filtrate, getting clean condensed liquid of the mono sialic acid hexose ganglioside sodium agent; sterilizing and getting the refine product. The invention changes the polarity of the extracting organic solvent, which makes the ganglioside be released almost fully into the solution. The invention employs the ionic sodium filter membrane to separate and converse the ganglioside depending on the its number difference in sialic acid and carboxyl groups, and employs modern membrane separation technique to replace filling material and column suction technique, which saves large quantity of solvent and time, and increases productivity.
Owner:严家定

Method for extracting alpha-glucosidase restraining agent effective ingredient from plants

The invention relates to a method for extracting effective ingredient as Alpha-glucosidase inhibitor, which belongs to the fields of Chinese herbal medicine preparation and chemical technology. The method comprises the following steps of: performing liquid extraction with organic solvent to remove ingredients with low Alpha-glucosidase inhibitory activity, and extracting effective ingredients with high Alpha-glucosidase inhibitory activity by using macroporous adsorptive resin and Sephadex LH-20 column chromatography technique. The yield of the inhibitor is 4%. The inventive method has the advantages of simple process, easy operation, high product purity, no harmful substances in product, and no environmental pollution. The obtained plant extract in the invention can be used for developing hypoglycemic drugs and further researching physical structure thereof.
Owner:TSINGHUA UNIV +1

Method for fast efficiently extracting and purifying anthocyanin from lonicera caerulea

The invention relates to a method for fast efficiently extracting and purifying cyanidin-3-O-glucoside from lonicera caerulea. The adopted technical scheme includes: taking a lonicera caerulea extract as a raw material, and adopting high-speed homogenized pulverized enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, low-temperature flocculent settling technology, medium-pressure quick-flash reverse column chromatographic technology and recrystallization technology to obtain refined cyanidin-3-O-glucoside having the purity more than 95%. The method is green and eco-friendly, less in steps, short in time consumption, less in loss of a target compound, high in product yield and purity, and suitable for massive production. The method has high economic value.
Owner:黑龙江伊蓝生物科技有限公司

Method for synthesizing (+/-)-9-O-demethyl-alpha-dihydrotetrabenazine

The invention discloses a method for synthesizing (+ / -)-9-O-demethyl-alpha-dihydrotetrabenazine, which comprises the following steps of: adding sodium hydride and hexamethylphosphoramide into dimethylbenzene, heating to the temperature of between 90 and 100 DEG C, dripping N-methylaniline, and stirring for 15 to 20 minutes; dripping dimethylbenzene suspension of (+ / -)-alpha-dihydrotetrabenazine into the mixed solution, after dripping, continuously stirring the reaction suspension at the temperature of between 90 and 100 DEG C for 48 to 50 hours; slowly adding aqueous solution of sodium hydroxide into the reaction suspension, separating out an aqueous phase, neutralizing the aqueous phase by using aqueous solution of hydrochloric acid until the pH value is 7 to 9, extracting by using diethyl ether, concentrating an organic phase under reduced pressure to obtain a light brown solid; and purifying the light brown solid by using column chromatography technology to obtain the target product. By optimizing the reaction conditions of the prior art and improving posttreatment and purification technology, the method has the advantages of low preparation cost, high repeatability and high yield.
Owner:JIANGSU INST OF NUCLEAR MEDICINE

Jujube root extract, extraction and separation method and application thereof

The invention belongs to the technical field of plant extraction and analysis, and especially relates to a jujube root extract, an extraction and separation method and an application thereof. The jujube root extract includes the following 15 components: ursolic acid, ceanothenic acid, eugenol-beta-D-glucoside, dihydrochalcone-4'-beta-D-glucopyranoside, oleanolic acid, 3-oxooleanolic acid, catechin, betulinic acid, jujube naphthoquinone, spineless common jujube benzyl II, beta-sitosterol, stigmasterol, daucosterol, p-hydroxybenzoic acid, and quercetin. The method utilizes ethanol extraction andethyl acetate extraction, and combines the use of silica gel, CHP-20P, macroporous resin, Sephadex LH-20 and other column chromatography techniques to separate the jujube root extract, and can be applied to researches of drugs for delaying Alzheimer's disease and treating diabetes.
Owner:NORTHWEST UNIV

Extraction method of radix glehniae acidic polysaccharide, extract thereof and application thereof in immunoregulation

The invention relates to a radix glehniae acidic polysaccharide extraction method, which specifically comprises: 1) adopting radix glehniae as a raw material, and carrying out a hot water extraction step, an ethanol precipitation step, a protein removal step, a dialysis concentration freeze-drying step to prepare radix glehniae crude polysaccharide; (2) extracting radix glehniae polysaccharide from the radix glehniae crude polysaccharide by adopting a DEAE (Diethylaminoethyl) SepharoseTM Fast Flow column chromatography technology; and (3) extracting the radix glehniae acidic polysaccharide from the radix glehniae polysaccharide by adopting a SephacrylTMS-300 High Response column chromatography technology, and indicating that the radix glehniae acidic polysaccharide has a lung cancer immunoregulation anti-tumor effect in a lung cancer immunoregulation anti-tumor experiment.
Owner:THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV

Novel ceramide compound with cell fading inducing activity and its preparing method

The present invention discloses a new kind of ceramide compound Altermides, which is extracted and separated from marine fungus Alternalia sp. fermenting liquid and has cell apoptosis activity. By means of the combined solvent extraction and column chromatography technology, one series of ceramide compounds is obtained from the fermenting liquid. The compounds have wide material source and is expected to become new anticancer medicine.
Owner:SHENYANG PHARMA UNIVERSITY

Preparation method of crisp pear tobacco flavor

The invention belongs to the technical field of tobacco flavors and particularly discloses a method for preparing a sweetening and moistening tobacco flavor from a Dangshan crisp pear as an extract by adopting an enzymolysis approach and a column chromatography technology. Through the operations of pulping, enzymolysis, column chromatography separation and eluting, vacuum concentration and the like, on the one hand, the juice yield of the Dangshan crisp pear is improved, a natural tobacco extract is reserved to the maximal extent, the aroma quality, the sweetness, liquid-engendering feeling and the taste softness are improved; and on the other hand, macromolecular substances affecting the sensory quality of the smoke are removed to obtain the high-quality extract. The crisp pear tobacco flavor prepared by the method is natural in aroma and distinct in fragrance characteristic, and is capable of playing a role in harmonizing the smoke, giving smoke sweetness, relieving throat irritation and improving the taste after being added to a cigarette at a proper ratio.
Owner:CHINA TOBACCO JIANGXI IND CO LTD

Method for preparing sotolon on basis of column chromatography separation and application to cigarettes

The invention provides a separation preparation method of sotolon and an application to cigarettes. Fenugreek seeds taken as the raw material are subjected to baking, extraction and concentration, a fenugreek tincture or extract is obtained, an organic solvent is taken as an eluent system, and the sotolon is obtained through silica-gel column chromatography separation. GC-MS detection indicates that GC purity of the sotolon is higher than 99%. The method is simple to operate, low in equipment requirement and shorter in consumed time.
Owner:CHINA TOBACCO GUANGXI IND

Dendrobium loddigesii rolfe Loddigesiinols G-K as well as preparation method and application thereof

The invention relates to dendrobium loddigesii rolfe Loddigesiinols G-K as well as a preparation method and an application thereof in preparation of hypoglycemic drugs. The Loddigesiinols G-K are prepared by the steps as follows: (1), an extracting solution obtained after dendrobium loddigesii rolfe is extracted by methyl alcohol is subjected to pressure-reduction concentration so as to obtain an extract; (2), the extract is sequentially extracted by petroleum ether, ethyl acetate and n-butyl alcohol; and (3), an ethyl acetate extract is repeatedly purified with a Sephadex LH-20 gel column chromatography technology and separated with an HPLC (high performance liquid chromatography) technology, so that five active Loddigesiinols G-K are obtained. Experiments show that dendrobium loddigesii rolfe Loddigesiinols G-K can be used as a hypoglycemic drug, a hyperglycemic health care product and a drug and health care product for preventing and curing cardiovascular disease.
Owner:SUN YAT SEN UNIV

Filefish skin collagen peptide having ACE restraining activity and preparation method of filefish skin collagen peptide

The invention relates to filefish skin collagen peptide having ACE restraining activity. The amino acid sequence of the collagen peptide is Gly-Val-Gla, and through UPLC-MS detection, the molecular ion peak is [M]+329m / z. The invention further relates to a preparation method of the collagen peptide. The preparation method comprises combined usage of mixing and hydrolysis through double enzymes, ultrafiltration and a gel column chromotography technique, the whole preparation process is simple and easy to control, the enzymolysis efficiency is high, and the obtained collagen peptide is safe andfree from side effects, has higher ACE restraining activity and also has better heat stability; besides, after being digested through digestive tracts of stomach and intestines, the collagen peptide still maintains higher ACE restraining activity; and in addition, processed waste namely fishskin is used as a raw material for preparing the collagen peptide, so that environmental pollution is effectively avoided, and the utilization rate of the resources is increased. The filefish skin collagen peptide has higher practical value and commercial value.
Owner:ZHEJIANG WANLI UNIV

Method for purifying bromelin by nano-titanium dioxide porous ceramic column chromatography

The invention discloses a method for purifying bromelin by a nano-titanium dioxide porous ceramic column chromatography, belonging to the technical field of enzyme separation and purification, mainly solving the problems of bromelin purification and enzymatic activity preservation. The technical scheme comprises the following steps of: manufacturing a porous ceramic column chromatography stuffing by a utilizing ceramic sintering technology; purifying the bromelin by adopting a column chromatography technology; and maximally preserving enzymatic activity through ultrafiltration and freeze-drying to obtain a finished product of the bromelin with the amount of activity units reaching 6 million. The invention is a high-efficiency and safe method for preparing the high-activity bromelin, and the product can be used as food or medicine raw material to be widely applied to the fields of food, biomedical industry and the like.
Owner:吴茂玉 +2

Separation and extraction method for gentiopicroside in Picrorhiza scrophulariiflora Pennell

Belonging to the technical field of chemical industry, the invention in particular relates to a separation and extraction method for gentiopicroside in Picrorhiza scrophulariiflora Pennell. The invention specifically employs extraction technique, macroporous resin column chromatography and silica gel column chromatography technique to elute a lot of gentiopicroside individually from a silica gel chromatographic column, thus increasing the extraction amount of gentiopicroside, and the finally obtained gentiopicroside has purity up to 98.7-99.3%, thus greatly improving the purity of gentiopicroside. The method provided by the invention has the advantages of wide application range, convenience and rapidity, low cost, simple operation and large separation amount, thereby being suitable for large-scale popularization and application.
Owner:CHONGQING MEDICAL & PHARMA COLLEGE

Jasmine essence for blasting bead for cigarette and preparation method and application of jasmine essence

The invention discloses jasmine essence for a blasting bead for a cigarette and a preparation method and application of the jasmine essence. The jasmine essence is prepared from, by mass, 10-20% of benzyl acetate, 2-10% of jasmonic acid methyl ester, 10-20% of Jasmine lactone, 3-6% of nerolidol, 15-25% of linalool, 10-20% of jasmine and 10-15% of benzyl alcohol. The cigarette with jasmine flower fragrance is prepared, raw materials required for preparing the jasmine essence are separated from extract of natural jasmine products and obtained, and the defect that synthetic essence only has the shape same with a natural perfume material and no essence of the natural perfume material is overcome. Through separation of a column chromatography technology, the mixed odor influence of jasmine extract and macromolecule substances of pectin, protein, fiber and the like in the essence on the sensory quality of the cigarette is reduced. The prepared blasting bead can reduce stimulation of the cigarette, mixed odor of the cigarette is reduced, the comfort degree of the cigarette is improved, and the cigarette has the jasmine fragrance characteristic.
Owner:CHINA TOBACCO HENAN IND

Semen plantaginis active part and preparation method thereof

The invention relates to a semen plantaginis active part and a preparation method thereof, and belongs to the technical field of extracts. The technical problems that in the prior art, chemical components of semen plantaginis are different in anti-oxidation effect, the direct oral administration availability is low, and clinical application is restricted are solved. The preparation method of the semen plantaginis active part comprises the following steps of firstly, mixing semen plantaginis with an ethanol solution, carrying out wall-breaking crushing, standing, carrying out heating reflux, and concentrating until the crude drug amount is 2.5-5.0 g / mL, so as to obtain a concentrated solution; and adsorbing the concentrated solution by using macroporous adsorption resin, washing, eluting byusing an ethanol solution with the concentration of 15vol%-70vol%, collecting the eluate, concentrating, and freeze-drying to obtain the active part of the semen plantaginis. According to the preparation method, the active part of the semen plantaginis is separated through a column chromatography technology, wherein the elution part of the ethanol solution with the concentration of 15vol%-70vol%has an anti-oxidation effect, and particularly the elution part of the ethanol solution with the concentration of 50vol% has the optimal effect.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Method for preparing India quassin compounds

The invention discloses a method for preparing India quassin compounds, which comprises the following steps of: using India quassia as raw materials, separating substances to be extracted out of the raw materials through tissue crushing, cellulase enzymolysis and ultrasonic extraction, conducting multi-stage countercurrent extraction by using n-butanol and water mixed solution and conducting separation through magnesia column chromatography to extract India quassin substances. The method has the characteristics of high extraction rate, simple operation process, low energy consumption, low cost and the like.
Owner:NANJING ZELANG MEDICAL TECH

Preparation method of imprinted polymers of core-shell type molecules, products of imprinted polymers of core-shell type molecules, and application of products

The invention relates to a preparation method of imprinted polymers of core-shell type molecules, products of the imprinted polymers of the core-shell type molecules, and the application of the products. The preparation method comprises the following steps: synthesizing a seed emulsion by adopting a monomeric compound shown as a formula II and a monomeric compound shown as a formula III; using the seed emulsion as a core, adopting the monomeric compound shown as the formula I and the monomeric compound shown as the formula II to carry out random copolymerization with the seed emulsion so as to obtain the imprinted polymers of the core-shell type molecules. According to the preparation method of the imprinted polymers of the core-shell type molecules, the products of the imprinted polymers of the core-shell type molecules, and the application of the products, which are disclosed by the invention, can be used for extracting lincomycin A with higher purity from lincomycin and analogues of the lincomycin; under the water-phase condition, or in a water solution, the lincomycin A, the lincomycin B and other analogous ingredient components can be separated, so that the lincomycin A with higher purity is obtained; a better separation result can be obtained when the preparation method is combined with the columns chromatography.
Owner:EAST CHINA UNIV OF SCI & TECH

Novel technology method for extracting alkaloids from wild menispermum dauricum

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting total alkaloids from wild menispermum dauricum in great khingan, in particular to a novel method for separating and purifying total alkaloids by using biological enzymolysis and ultrahigh pressure extraction, and combining with column chromatography. The method has the advantages that 1, a biological enzymolysis technique is adopted, so that effective components of alkaloids in cells are dissolved out fully; 2, an ultrahigh pressure extraction technique is adopted, so that the extraction time can be shortened; the energy consumption can be reduced; impurity components can be prevented from being dissolved out; the yield of the effective components is increased; the purity is high; and 3, with the adoption of a column chromatography technique for adsorption and separation, extracted total alkaloids are further purified. The method adopts the biological enzymolysis method, so that the effective components are dissolved out fully; then the ultrahigh pressure extraction and the column chromatography are adopted for separation and purification; and the purity of obtained total alkaloids is increased to 93.6%.
Owner:DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD

Ligusticum wallichii homogeneous polysaccharide as well as extraction method and application thereof

The invention provides ligusticum wallichii polysaccharide and an extraction method and application thereof. The extraction method includes the specific steps that ligusticum wallichii serves as a rawmaterial, and crude ligusticum wallichii polysaccharide is prepared through a hot water extraction step, an ethanol precipitation step, a protein removal step and a dialysis concentration freeze drying step; ligusticum wallichii polysaccharide is extracted from crude ligusticum wallichii polysaccharide by adopting a DEAE SepharoseTMFast Flow column chromatography technology; the ligusticum wallichii polysaccharide is extracted by adopting a SephacrylTMS-300High Resolution column chromatography technology to obtain two homogeneous polysaccharides LCXP-1a and LCXP-3a, experiments of comparingthe two ligusticum wallichii homogeneous polysaccharides in liver cancer immunoregulation show that the ligusticum wallichii homogeneous polysaccharides have immunoregulation and anti-tumor effects.
Owner:THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV

Marine-source antifreeze peptide preparation as well as preparation method and application thereof

The invention relates to a marine source antifreeze peptide preparation, the antifreeze peptide preparation contains polypeptide His-Val-Arg-Gly-Ala-Phe-Ser-Glu-Glu, the polypeptide accounts for 60-70% of the total content of the antifreeze peptide preparation, the molecular ion peak of the antifreeze peptide preparation is [M] + 1052 m / z through UPLC-MS detection, and the antifreeze peptide preparation can play a good low-temperature protection role on escherichia coli and catalase. The invention also relates to a preparation method of the marine-source antifreeze peptide preparation, the method combines a protease enzymolysis technology and a gel column chromatography technology, the operation is simple, the cost is low, the enzymolysis process efficiency is high, and the antifreeze peptide preparation obtained by separation and purification has high antifreeze activity; the adopted raw material is the squid skin of a processing byproduct, so that the utilization degree of resources can be effectively improved, and the environmental pollution is reduced.
Owner:ZHEJIANG WANLI UNIV
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