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Method for preparing compound of vegetative phosphatidylinositol in high purity

A phosphatidylinositol and purity technology, which is applied in the field of preparation of high-purity plant phosphatidylinositol compounds, can solve the problem that there is no large-scale preparation of high-purity PI, no use of animals, and no large-scale preparation of high-purity PI compounds. problems, to achieve the effects of significant anti-tumor biological activity, low pollution, and scientific process design methods

Inactive Publication Date: 2006-02-01
商宗一
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, in the research patents of PI, there is no patent for the method of preparing high-purity PI in large quantities
[0008] For more than 20 years, almost all PI compounds used in scientific research, whether natural or chemically synthesized, are purchased from large biochemical reagent companies. It is known that PI compounds are commercially available using preparative TLC or equivalent dry column chromatography techniques , and then obtain a small amount of product (mg level) through separation and purification, which is limited to the scientific research of cancer cells and brain cells. So far, there is no technology for large-scale preparation of high-purity PI compounds, nor is it used in animals, and there are no reports of oral experiments.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1. 1Kg of high-quality black soybean powder, add chloroform: methanol: water (1: 1: 1v / v), (1: 2: 1v / v), 5-20 times the volume, soak for 24-72 hours below 50°C , separate the extract, and concentrate under reduced pressure after layering. If the solvent is gray, you can add pharmaceutical activated carbon (3-5g) to reflux for one hour, decolorize, filter, and then concentrate under reduced pressure. Recover the solvent to obtain black soybean total Phospholipids (about 85% pure).

Embodiment 2

[0016] Example 2. High-quality soybean powder 1Kg, add chloroform: methanol: water (1: 1: 1v / v), (1: 2: 1v / v), 5-20 times the volume, soak for 24-72 hours below 50°C , separate the extract, concentrate under reduced pressure after layering, pass through the filter, concentrate under reduced pressure again, and recover the solvent to obtain soybean total phospholipids (purity about 85%). The yield of soybeans is higher than that of black beans.

[0017] 2. Preparation of PI compound boutique:

Embodiment 3

[0018] Embodiment 3. The primary pure product is further refined:

[0019] The present invention is based on the composition, structure and chemical properties of the total phospholipids of PI compounds. In order to further improve the purity of PI, a novel macroporous resin column chromatography technology is selected for the first time, including macroporous strong-acid resins, and macroporous weak-base resins can also be used. , for further purification, the solvent is chloroform:methanol (1:1v / v), (1:2v / v), or (2:1v / v); the eluent is concentrated under reduced pressure, vacuum After drying, it can be effectively separated and purified to obtain the pure product of PI compound with a purity greater than 96%. The PI yield per Kg of soybean flour is greater than 500mg, and the cost per gram is about 200 RMB.

[0020] Identification method: take the PI pure product obtained by the above method, and conduct a control experiment with the PI pure product of SIGMA Company, using ...

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PUM

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Abstract

A process for preparing the high-purity (more than 96%) vegetative phosphatidylinositol (PI) from soybean includes such steps as extracting in solvent, separating, and purifying by macroreticular resin chromatography. Said PI has high activity of selectively killing more kinds of human cancer cells and preventing and treating senile dementia.

Description

technical field [0001] The present invention uses leguminous plant seeds as raw materials to establish a set of simple and effective production techniques for extracting, separating and purifying, and preparing high-purity plant phosphatidylinositol (Phosphatidylinositol (abbreviated as PI) in large quantities, and the compound prepared by this technique, to prepare Injection and oral liquid emulsion, microcapsule or liposome. [0002] We are the first at home and abroad to conduct anti-tumor injection and oral tests of PI in tumor-bearing animals, and combined with cyclophosphamide and paclitaxel, all of which have achieved very significant anti-tumor effects (see attached table 1, 2, 3). Our many years of scientific research and practice have proved that the production method of the present invention is correct and reasonable, and the product is safe and effective. Background technique [0003] Plant PI is one of the most important components of phospholipids. It is not ...

Claims

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Application Information

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IPC IPC(8): C07F9/10A61K31/66A61K9/107A61K9/127A61K9/50A61P35/00
Inventor 商宗一
Owner 商宗一
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