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Ligusticum wallichii homogeneous polysaccharide as well as extraction method and application thereof

An extraction method, the technology of polysaccharides from Rhizoma Chuanxiong, applied in the field of homogeneous polysaccharides from Rhizoma Chuanxiong and its extraction, can solve the problems of low purity, no exploration of biological activity, failure to detect molecular weight and monosaccharide composition, etc., and achieve high purity and strong immune activity Effect

Inactive Publication Date: 2020-10-16
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Their shortcoming is that there is no further separation and purification by column chromatography, the extracted polysaccharides of Ligusticum chuanxiong are not homogeneous polysaccharides, the purity is low, their molecular weight and monosaccharide composition cannot be detected, and their biological activity has not been explored.

Method used

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  • Ligusticum wallichii homogeneous polysaccharide as well as extraction method and application thereof
  • Ligusticum wallichii homogeneous polysaccharide as well as extraction method and application thereof
  • Ligusticum wallichii homogeneous polysaccharide as well as extraction method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1: the extraction of the homogeneous polysaccharide of Rhizoma Chuanxiong, such as figure 1 , figure 2 , image 3 , Figure 4 shown.

[0076] Product 1:

[0077] A kind of extraction of Rhizoma Chuanxiong homogeneity polysaccharide, concrete steps are as follows:

[0078] 1) using Rhizoma Chuanxiong as a raw material, crudely extracting crude polysaccharides from Rhizoma Chuanxiong;

[0079] 1-1) Hot water extraction step: crush the raw material Chuanxiong in a grinder to obtain Chuanxiong powder; add Chuanxiong powder into the hot water extraction reaction cup, then add distilled water Ⅰ, stir in a 75°C water bath for 1.5h, and collect by filtration Extract solution I; add distilled water II to the residue, place in a water bath at 75°C and stir for 1.5 h, collect extract solution II by filtration; combine extract solution I and extract solution II, centrifuge to remove precipitate, and concentrate under reduced pressure on a rotary evaporator. The wa...

Embodiment 2

[0128] Embodiment 2: the chemical composition of Chuanxiong homogeneous polysaccharide LCXP-1a and LCXP-3a:

[0129] 1. Experimental materials:

[0130] Ligusticum chuanxiong homogeneous polysaccharide LCXP-1a and LCXP-3a, sodium tetraborate, m-hydroxybiphenyl, galacturonic acid, concentrated sulfuric acid, sodium hydroxide, phenol obtained under the conditions of product 3 in embodiment 1

[0131] 2. Experimental method:

[0132] 2-1. Detection of polysaccharide content: using phenol-sulfuric acid method

[0133] Dissolve 10mg of LCXP-1a and LCXP-3a polysaccharide samples in 1000ml of distilled water, mix well, take 2ml into a test tube, first add 1ml of phenol solution (1g of phenol is dissolved in 20ml of distilled water), mix well, then add 5ml of concentrated sulfuric acid and mix well After cooling, measure the absorbance at a wavelength of 490nm, and substitute the measured absorbance into the standard curve to calculate the polysaccharide content.

[0134] 2-2. Dete...

Embodiment 3

[0139] Embodiment 3: Molecular weight and purity of Chuanxiong homogeneous polysaccharide LCXP-1a and LCXP-3a:

[0140] 1. Experimental materials:

[0141] Ligusticum chuanxiong homogeneity polysaccharide LCXP-1a and LCXP-3a, NaNO made by the condition of product 3 in embodiment 1 3 NaN 3 , narrow distribution polyethylene glycol (PEG)

[0142] 2. Experimental method:

[0143] LCXP-1a and LCXP-3a were loaded with high performance gel permeation chromatography HPGPC system (Japan Shimadzu LC20 gel permeation chromatography; Japan TOSOH company TSKgel GMPWXL aqueous gel chromatographic column) analysis, with differential detector (Shimadz Corporation, Japan, RID-20a) to record the results. The mobile phase is 0.1N NaNO 3 +0.06%NaN 3 Aqueous solution, mobile phase flow rate 0.6mL / min, column temperature 35°C. The standard sample is narrow-distribution polyethylene glycol (PEG), and the narrow-distribution polyethylene glycol PEG is used as the standard curve, and the relat...

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Abstract

The invention provides ligusticum wallichii polysaccharide and an extraction method and application thereof. The extraction method includes the specific steps that ligusticum wallichii serves as a rawmaterial, and crude ligusticum wallichii polysaccharide is prepared through a hot water extraction step, an ethanol precipitation step, a protein removal step and a dialysis concentration freeze drying step; ligusticum wallichii polysaccharide is extracted from crude ligusticum wallichii polysaccharide by adopting a DEAE SepharoseTMFast Flow column chromatography technology; the ligusticum wallichii polysaccharide is extracted by adopting a SephacrylTMS-300High Resolution column chromatography technology to obtain two homogeneous polysaccharides LCXP-1a and LCXP-3a, experiments of comparingthe two ligusticum wallichii homogeneous polysaccharides in liver cancer immunoregulation show that the ligusticum wallichii homogeneous polysaccharides have immunoregulation and anti-tumor effects.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to the homogeneous polysaccharide of Rhizoma Chuanxiong and its extraction method and application. Background technique [0002] Chuanxiong is a traditional Chinese medicine with a long history. In recent years, various biological activities of Chuanxiong have been widely studied, mainly including anti-inflammatory, cardiovascular effects, slowing down cell aging, anti-oxidation and anti-tumor activities in vitro. At present, more than 170 components have been isolated from Chuanxiong, including phthalates, terpenes and their enols, alkaloids, polysaccharides, organic acids and their esters, etc. Ligusticum chuanxiong polysaccharides (LCXP) is one of the main biologically active substances of Ligusticum chuanxiong. The research on LCXP is gradually increasing, usually including the extraction, separation, purification and biological activity of LCXP. Studies have shown tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/02A61P1/16
CPCA61K31/715A61P1/16A61P35/00A61P37/02C08B37/0003C08B37/006
Inventor 鲍依稀钟诚刘子靖张旭裕蒲有为
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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