Novel technology method for extracting alkaloids from wild menispermum dauricum

A technology of bat kudzu and total alkaloids, applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of high temperature, long operation time, unstable yield, etc., achieve no chemical residue and increase yield , the effect of high product purity

Inactive Publication Date: 2013-04-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In China, organic solvent extraction and acidic aqueous solution extraction are generally used to extract alkaloids, which have disadvantages such as long operating time, high temperature, and unstable yield. The method of chromatographically purifying alkaloids has high product purity and high yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A method for obtaining high-purity total alkaloids by using biological enzymatic hydrolysis, ultra-high pressure extraction combined with column chromatography separation and purification, the steps are as follows:

[0019] (1) Biological enzymolysis: 100g of dried Pueraria puerariae root was crushed to 20 mesh, the enzyme concentration was 0.8g / l, the enzymolysis temperature was 45°C, and the enzymolysis time was 3h to obtain a mixture;

[0020] (2) Pretreatment:

[0021] The solvent and the raw material ratio are mixed in a ratio of 50:1, and the concentration of ethanol added is 50%, packed in a packaging bag, and sealed;

[0022] (3) Ultra-high pressure extraction:

[0023] Put the packaging bag into an ultra-high pressure container, and process it for 3 minutes under the ultra-high pressure of 200MPA and room temperature;

[0024] (4) get crude product:

[0025] The resulting liquid was vacuum-dried at 60°C to obtain crude total alkaloids;

[0026...

Embodiment example 2

[0029] Implementation Case 2: A method for obtaining high-purity total alkaloids by using biological enzymatic hydrolysis, ultra-high pressure extraction combined with column chromatography separation and purification, the steps are as follows:

[0030] (1) Biological enzymolysis: 500g of dried Pueraria puerariae root was crushed to 40 mesh, the enzyme concentration was 1.0g / l, the enzymolysis temperature was 50°C, and the enzymolysis time was 3.5h to obtain a mixture;

[0031] (2) Pretreatment:

[0032] The ratio of solvent and raw material is mixed according to 60:1, and the concentration of ethanol is added to be 60%, and the packaging bag is packed and sealed;

[0033] (3) Ultra-high pressure extraction:

[0034] Put the packaging bag in an ultra-high pressure container, and treat it at room temperature for 4 minutes under an ultra-high pressure of 300MPA;

[0035] (4) get crude product:

[0036] The resulting liquid was vacuum-dried at 60°C to obtain a crude alkaloid; ...

Embodiment example 3

[0040] Implementation Case 3: A method for obtaining high-purity total alkaloids by using biological enzymatic hydrolysis, ultra-high pressure extraction combined with column chromatography separation and purification, the steps are as follows:

[0041] (1) Biological enzymolysis: 200g of dried Pueraria puerariae root was crushed to 40 mesh, the enzyme concentration was 1.2g / l, the enzymolysis temperature was 50°C, and the enzymolysis time was 4h to obtain a mixture;

[0042] (2) Pretreatment:

[0043] The ratio of solvent and raw material is mixed according to the ratio of 70:1, and the concentration of ethanol is added to be 70%, and the packaging bag is packed and sealed;

[0044] (3) Ultra-high pressure extraction:

[0045] Put the packaging bag in an ultra-high pressure container, and process it at an ultra-high pressure of 400MPA and room temperature for 5 minutes;

[0046] (4) get crude product:

[0047] The resulting liquid was vacuum-dried at 60°C to obtain a crude...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting total alkaloids from wild menispermum dauricum in great khingan, in particular to a novel method for separating and purifying total alkaloids by using biological enzymolysis and ultrahigh pressure extraction, and combining with column chromatography. The method has the advantages that 1, a biological enzymolysis technique is adopted, so that effective components of alkaloids in cells are dissolved out fully; 2, an ultrahigh pressure extraction technique is adopted, so that the extraction time can be shortened; the energy consumption can be reduced; impurity components can be prevented from being dissolved out; the yield of the effective components is increased; the purity is high; and 3, with the adoption of a column chromatography technique for adsorption and separation, extracted total alkaloids are further purified. The method adopts the biological enzymolysis method, so that the effective components are dissolved out fully; then the ultrahigh pressure extraction and the column chromatography are adopted for separation and purification; and the purity of obtained total alkaloids is increased to 93.6%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting total alkaloids from Daxinganling wild bat Geli, in particular to a new method for purifying total alkaloids by utilizing biological enzymolysis, ultra-high pressure extraction and column chromatography technology. Background technique: [0002] Bat kudzu, also known as mountain bean root, is a winding deciduous woody vine with a length of more than 10 meters. The rhizome is slender, the outer skin often falls off in layers or flakes, the section is yellowish white, and the taste is extremely bitter. Branchlets green, alternate leaves, reniform or ovoid, 5-12cm long, entire or 3-7 lobed, with petioles peltate. Flowers are unisexual, short panicles axillary, small flowers, small yellow-green flowers in summer, flowering period from May to June. The drupe is nearly spherical, purple-black when ripe, and the fruit period is July-September. The rhizo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G5/00
Inventor 张亚红姚德坤姚德利万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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