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Method for preparing India quassin compounds

A technology of bitternin and compounds, which is applied in the field of preparation of Indian bitternin compounds, and achieves the effects of high extraction rate, low energy consumption and low cost

Inactive Publication Date: 2012-12-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the preparation process applicable to the industrialized large-scale production of Indian picrophyllin compounds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 2 kg of Indian bitter wood stems and cut them into small pieces, then put them into a high-speed tissue homogenizer, keep them at 10,000 rpm for 10 minutes, add 6 kg of water, and then add 0.5% cellulase, and then put them into a high-speed tissue homogenizer and grinder. Enzyme hydrolyze for 30min under the conditions, then dry at 65°C, add 8L of 60% ethanol to heat and reflux for extraction once, extract for 2h, collect the extract, filter, recover ethanol from the filtrate under reduced pressure, and use n-butanol with a volume ratio of 10:1 -The water mixed solution is subjected to 5-stage countercurrent extraction, the extract is combined, the reagent is recovered under reduced pressure, and the concentrated solution is added to the magnesium oxide column chromatography (the ratio of the concentrated solution to the magnesium oxide is 1:3), and the volume of n-hexane-ethyl acetate Gradient elution was carried out at a ratio of 5:1, 3:1, 1:1, and 1:3, and the am...

Embodiment 2

[0016] Take 2 kg of Indian bitter wood stems and cut them into small pieces, then put them into a high-speed tissue homogenizer, keep them at 10,000 rpm for 10 minutes, add 6 kg of water, and then add 0.5% cellulase. Enzymolysis for 30min under the conditions, then drying at 70°C, adding 10L of 70% ethanol to heat and reflux extraction for 3 times, extracting for 1.5h, collecting the extract, filtering, recovering the ethanol from the filtrate under reduced pressure, and using n-butane with a volume ratio of 10:1 The alcohol-water mixed solution is subjected to 6-stage countercurrent extraction, the extracts are combined, the reagents are recovered under reduced pressure, and the concentrated solution is added to the magnesium oxide column chromatography (the ratio of the concentrated solution to magnesium oxide is 1:5), according to the ratio of n-hexane-ethyl acetate Gradient elution with volume ratios of 5:1, 3:1, 1:1, and 1:3, with 400ml for each gradient, collecting and me...

Embodiment 3

[0018] Take 2 kg of Indian bitter wood stems and cut them into small pieces, then put them into a high-speed tissue homogenizer, keep them at 10,000 rpm for 10 minutes, then add 10 kg of water, then add 0.5% cellulase, and put them into a high-speed tissue homogenizer at 400W, 35°C Enzyme hydrolyze for 30min under the conditions, then dry at 75°C, add 6L of 65% ethanol to heat and reflux to extract twice, extract for 2h, collect the extract, filter, and recover the ethanol from the filtrate under reduced pressure, and use n-butanol with a volume ratio of 10:1 -The water mixed solution is subjected to 5-stage countercurrent extraction, the extract is combined, the reagent is recovered under reduced pressure, and the concentrated solution is added to the magnesium oxide column chromatography (the ratio of the concentrated solution to the magnesium oxide is 1:4), and the volume of n-hexane-ethyl acetate Gradient elution was carried out at a ratio of 5:1, 3:1, 1:1, and 1:3, and the...

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Abstract

The invention discloses a method for preparing India quassin compounds, which comprises the following steps of: using India quassia as raw materials, separating substances to be extracted out of the raw materials through tissue crushing, cellulase enzymolysis and ultrasonic extraction, conducting multi-stage countercurrent extraction by using n-butanol and water mixed solution and conducting separation through magnesia column chromatography to extract India quassin substances. The method has the characteristics of high extraction rate, simple operation process, low energy consumption, low cost and the like.

Description

Technical field [0001] The present invention is a method of preparing Indian Burinin compounds in the field of traditional Chinese medicine and chemistry. Background technique [0002] Simaroubaceae plants, mainly distributed in tropical and subtropical regions.There are 4 genus and about 10 species in my country. It is distributed in all provinces in the south of the Yangtze River. It is an important type of medicinal plant resources.The chemical components contained in this family of this family are mainly QuasSinoids and alkaloids, followed by trioma, sterin, saponin, fragrant beanins, and crickets.Indian Bitewood Quassia Indica (Gartn.) NOOTEBOOM is separated from the Bitein compounds in Indian Bite and Indian Burnin X.Indian Kitanin C and X have cytotoxicity, in vitro to KB cell IC 50 It is 1.00 μg / ml, 0.60 μg / ml; Indian Burnite X also has anti -tumor activity, and the concentration is 10 μg / ml at all times to inhibit neutral white blood cell adhesion to endothelial cells in...

Claims

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Application Information

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IPC IPC(8): C07D493/10
Inventor 刘东锋
Owner NANJING ZELANG MEDICAL TECH
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