Marine-source antifreeze peptide preparation as well as preparation method and application thereof
A marine and squid skin technology, applied in the field of marine source antifreeze peptide preparation and its preparation, achieves the effect of low cost, simple and easy to control the preparation process
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Embodiment 1
[0043] (1) Pretreatment of squid skin
[0044] A: Peel off the impurities on the squid skin, wash and drain repeatedly;
[0045] B: take by weighing the drained squid skin of step (A) gained, add 1mol / L sodium chloride solution according to the material-to-liquid ratio 1:20, make squid skin soak in sodium chloride solution for 12h, replace sodium chloride every 6h solution, the soaked squid skin is washed with distilled water;
[0046] C: take by weighing the squid skin of step (B) gained, add 10% isopropanol solution by material-liquid ratio 1:10, make squid skin soak in isopropanol solution for 18h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.
[0047] (2) Preparation of squid skin collagen
[0048] Mincing the squid skin obtained in step (1), adding pH3 citric acid buffer to the minced squid skin at a material-to-liquid ratio of 1:100 to form a suspension, and placing the suspension in a 60°C water bath for extraction ...
Embodiment 2
[0054] (1) Pretreatment of squid skin
[0055] A: Peel off the impurities on the squid skin, wash and drain repeatedly;
[0056] B: take by weighing the drained squid skin of step (A) gained, add 5mol / L sodium chloride solution by material-liquid ratio 1:30, make squid skin soak in sodium chloride solution for 36h, replace sodium chloride every 6h solution, the soaked squid skin is washed with distilled water;
[0057] C: take by weighing the squid skin of step (B) gained, add 25% isopropanol solution by material-to-liquid ratio 1:25, make squid skin soak in isopropanol solution for 24h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.
[0058] (2) Preparation of squid skin collagen
[0059] Mincing the squid skin obtained in step (1), adding pH5 citric acid buffer to the minced squid skin at a material-to-liquid ratio of 1:200 to form a suspension, and then placing the suspension in a water bath at 80°C for extraction 8h, wh...
Embodiment 3
[0065] (1) Pretreatment of squid skin
[0066] A: Peel off the impurities on the squid skin, wash and drain repeatedly;
[0067] B: take by weighing the drained squid skin of step (A) gained, add 2.5mol / L sodium chloride solution according to the material-to-liquid ratio 1:25, make squid skin soak in sodium chloride solution for 24h, replace the chloride every 6h Sodium solution, soaked squid skin is washed with distilled water;
[0068] C: take by weighing the squid skin of step (B) gained, add 15% isopropanol solution by material-liquid ratio 1:15, make squid skin soak in isopropanol solution for 18h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.
[0069] (2) Preparation of squid skin collagen
[0070] The squid skin obtained in step (1) is minced, and pH4 citric acid buffer is added to the minced squid skin according to a material-to-liquid ratio of 1:100 to form a suspension, and then the suspension is placed in a 60°C ...
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