Marine-source antifreeze peptide preparation as well as preparation method and application thereof

A marine and squid skin technology, applied in the field of marine source antifreeze peptide preparation and its preparation, achieves the effect of low cost, simple and easy to control the preparation process

Active Publication Date: 2022-05-27
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Squid skin accounts for the largest proportion of squid processing by-products. Squid skin is rich in collagen, accounting for about 70% of its dry weight. It is a good source of collagen. Studies have shown that squid skin collagen peptides have anti-oxidation, anti-cancer, anti- Decreased, hypoglycemic, blood pressure and other functions, but there is no report on the preparation of collagen antifreeze peptides from squid skin in the existing technology

Method used

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  • Marine-source antifreeze peptide preparation as well as preparation method and application thereof
  • Marine-source antifreeze peptide preparation as well as preparation method and application thereof
  • Marine-source antifreeze peptide preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Pretreatment of squid skin

[0044] A: Peel off the impurities on the squid skin, wash and drain repeatedly;

[0045] B: take by weighing the drained squid skin of step (A) gained, add 1mol / L sodium chloride solution according to the material-to-liquid ratio 1:20, make squid skin soak in sodium chloride solution for 12h, replace sodium chloride every 6h solution, the soaked squid skin is washed with distilled water;

[0046] C: take by weighing the squid skin of step (B) gained, add 10% isopropanol solution by material-liquid ratio 1:10, make squid skin soak in isopropanol solution for 18h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.

[0047] (2) Preparation of squid skin collagen

[0048] Mincing the squid skin obtained in step (1), adding pH3 citric acid buffer to the minced squid skin at a material-to-liquid ratio of 1:100 to form a suspension, and placing the suspension in a 60°C water bath for extraction ...

Embodiment 2

[0054] (1) Pretreatment of squid skin

[0055] A: Peel off the impurities on the squid skin, wash and drain repeatedly;

[0056] B: take by weighing the drained squid skin of step (A) gained, add 5mol / L sodium chloride solution by material-liquid ratio 1:30, make squid skin soak in sodium chloride solution for 36h, replace sodium chloride every 6h solution, the soaked squid skin is washed with distilled water;

[0057] C: take by weighing the squid skin of step (B) gained, add 25% isopropanol solution by material-to-liquid ratio 1:25, make squid skin soak in isopropanol solution for 24h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.

[0058] (2) Preparation of squid skin collagen

[0059] Mincing the squid skin obtained in step (1), adding pH5 citric acid buffer to the minced squid skin at a material-to-liquid ratio of 1:200 to form a suspension, and then placing the suspension in a water bath at 80°C for extraction 8h, wh...

Embodiment 3

[0065] (1) Pretreatment of squid skin

[0066] A: Peel off the impurities on the squid skin, wash and drain repeatedly;

[0067] B: take by weighing the drained squid skin of step (A) gained, add 2.5mol / L sodium chloride solution according to the material-to-liquid ratio 1:25, make squid skin soak in sodium chloride solution for 24h, replace the chloride every 6h Sodium solution, soaked squid skin is washed with distilled water;

[0068] C: take by weighing the squid skin of step (B) gained, add 15% isopropanol solution by material-liquid ratio 1:15, make squid skin soak in isopropanol solution for 18h, replace isopropanol solution every 6h, soak After the squid skin is washed with distilled water.

[0069] (2) Preparation of squid skin collagen

[0070] The squid skin obtained in step (1) is minced, and pH4 citric acid buffer is added to the minced squid skin according to a material-to-liquid ratio of 1:100 to form a suspension, and then the suspension is placed in a 60°C ...

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Abstract

The invention relates to a marine source antifreeze peptide preparation, the antifreeze peptide preparation contains polypeptide His-Val-Arg-Gly-Ala-Phe-Ser-Glu-Glu, the polypeptide accounts for 60-70% of the total content of the antifreeze peptide preparation, the molecular ion peak of the antifreeze peptide preparation is [M] + 1052 m/z through UPLC-MS detection, and the antifreeze peptide preparation can play a good low-temperature protection role on escherichia coli and catalase. The invention also relates to a preparation method of the marine-source antifreeze peptide preparation, the method combines a protease enzymolysis technology and a gel column chromatography technology, the operation is simple, the cost is low, the enzymolysis process efficiency is high, and the antifreeze peptide preparation obtained by separation and purification has high antifreeze activity; the adopted raw material is the squid skin of a processing byproduct, so that the utilization degree of resources can be effectively improved, and the environmental pollution is reduced.

Description

technical field [0001] The present invention relates to antifreeze peptides, in particular to a marine antifreeze peptide preparation and its preparation method and application. Background technique [0002] Antifreeze peptides are a class of specific polypeptides or glycopeptides produced by organisms to adapt to extreme cold environments. It is able to decrease the ice crystal growth point in a non-collateral manner without affecting its melting point to inhibit ice crystal growth and recrystallization. In 1969, Devries first discovered the existence of AFPs in the blood of Antarctic fish, and then AFPs were found in other species. At present, the existence of AFP has been found in polar organisms, wintering organisms, cold-resistant plants, bacteria, fungi, animals and other organisms. [0003] Collagen has a unique triple helix structure, and a large number of studies have shown that collagen contains polypeptides with antifreeze activity. The collagen polypeptide cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14C07K1/16C12P21/06
CPCC07K14/78C12P21/06Y02A40/81
Inventor 戚向阳曹少谦蔡金秀马佳雯杨华
Owner ZHEJIANG WANLI UNIV
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