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Jujube root extract, extraction and separation method and application thereof

A separation method and extraction technology, applied in the separation/purification of carboxylic acid compounds, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the unreported problems of jujube root chemical composition and pharmacological activity, etc. To achieve the effect of delaying the treatment of diabetes and Alzheimer's disease

Active Publication Date: 2018-12-14
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the researches on the chemical constituents and pharmacological effects of Jujube phytochemical components and pharmacological effects are mostly on the fruits and branches and leaves, but the chemical constituents and pharmacological activities of Jujube roots have not been reported yet.

Method used

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  • Jujube root extract, extraction and separation method and application thereof
  • Jujube root extract, extraction and separation method and application thereof
  • Jujube root extract, extraction and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting and separating jujube root extract, comprising the following steps:

[0026] S1, crush the jujube root through a 40-mesh sieve, extract twice with an ethanol solution with a volume concentration of 90%, soak for 48 hours each time and then start to leak, combine the two extracts and concentrate under reduced pressure to obtain an extract. details as follows:

[0027] 4.7kg of jujube roots were crushed through a 40-mesh sieve, extracted with 60L of ethanol solution with a volume concentration of 90%, soaked for 48 hours each time, and then began to infiltrate, and the filtrate and filter residue were collected once, and the filtrate was used as the extraction solution once. The filter residue was extracted once more by percolation according to the above procedure, and the two extracts were combined and concentrated under reduced pressure at 45°C and 0.09Mpa to obtain extract (293.60g).

[0028] S2, add water to dissolve the extract in S1 to obtai...

Embodiment 2

[0042] An analytical method for 6 substances in the above-mentioned jujube root extract, comprising the steps of:

[0043] 1. Purchase the standard products of each substance: dihydrochalcone-4′-β-D-glucoside (1), eugenol-β-D-glucoside (2), aspinoside II (3) , p-Hydroxybenzoic acid (4), catechin (5), quercetin (6), prepared into a series of concentrations of 10mg / L, 40mg / L, 80mg / L, 150mg / L, 200mg / L with methanol Standard solution, stored in a refrigerator at 4°C until use.

[0044] 2. Use high-performance liquid chromatography to detect 6 kinds of standard solutions, choose different mobile phase gradient elution methods, and constantly adjust the chromatographic conditions according to the detection structure to determine the best chromatographic conditions: Chromatographic column: Luna C-18 Column (5μm, 250×4.60mm, Phenomenex Corporation, Torrance, California, USA); mobile phase A: 0.2% phosphoric acid-water, mobile phase B: acetonitrile; gradient elution program: 0~10min, ...

Embodiment 3

[0056] Application of jujube root extract of the present invention in preparation of drugs for delaying Alzheimer's disease and treating diabetes. In order to verify its efficacy, the following experiments were carried out:

[0057] 1. Acetylcholinesterase activity inhibition experiment

[0058] Assay method: the extract of Example 1 was dissolved in DMSO to form a solution with a concentration of 1.0 mg / mL. In a 96-well plate, 140 μL of 0.1mol / L phosphate buffer (pH 7.4), 20 μL test sample and 20 μL acetylcholinesterase, as sample group; 160 μL 0.1mol / L phosphate buffer (pH is 7.4) and 20 μL test sample were added to 24 wells in columns 4, 5 and 6 as control group; 160 μL 0.1mol / L phosphate buffer (pH 7.4) and 20 μL acetylcholinesterase were added to the 3 wells of columns 7, 8 and 9 as the blank group; after incubation at 4°C for 20 min, 10 μL of 15 mM ACTI and 10 μL of 2mM DTNB, and then incubated at 37°C for 20 minutes, and then measured the absorbance value at 412nm wav...

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Abstract

The invention belongs to the technical field of plant extraction and analysis, and especially relates to a jujube root extract, an extraction and separation method and an application thereof. The jujube root extract includes the following 15 components: ursolic acid, ceanothenic acid, eugenol-beta-D-glucoside, dihydrochalcone-4'-beta-D-glucopyranoside, oleanolic acid, 3-oxooleanolic acid, catechin, betulinic acid, jujube naphthoquinone, spineless common jujube benzyl II, beta-sitosterol, stigmasterol, daucosterol, p-hydroxybenzoic acid, and quercetin. The method utilizes ethanol extraction andethyl acetate extraction, and combines the use of silica gel, CHP-20P, macroporous resin, Sephadex LH-20 and other column chromatography techniques to separate the jujube root extract, and can be applied to researches of drugs for delaying Alzheimer's disease and treating diabetes.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and analysis, and in particular relates to a jujube root extract, an extraction and separation method and an application thereof. Background technique [0002] Jujube, aliased as dried jujube, red jujube, jujube, big jujube, good jujube, thorn jujube, and perennial jujube, is the dry and mature fruit of the plant jujube (Ziziphus jujuba Mill.), belonging to the Rhamnaceae (Rhamnaceae) genus (Ziziphus Mill. ) plant. It was first recorded in the first Chinese pharmacy monograph "Shen Nong's Materia Medica", and was listed as the top grade of all medicines. Jujube has a long history and is listed as one of my country's "five fruits" (peach, plum, chestnut, apricot, jujube). It is not only a common food, but also a traditional Chinese medicine. Stomach, Heart Sutra. [0003] Jujube tree, more than 10 meters high, is a small deciduous tree and a sparse shrub. The bark is brown or taupe, and...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/203C07H1/08C07D311/62C07D311/30C07D311/40C07D305/14C07H15/18C07J9/00C07J17/00C07C51/42C07C65/05A61P3/10A61P25/28A61K31/56A61K31/7034A61K31/353A61K31/337A61K31/7028A61K31/575A61K31/704A61K31/352A61K31/192
CPCA61K31/192A61K31/337A61K31/352A61K31/353A61K31/56A61K31/575A61K31/7028A61K31/7034A61K31/704C07C51/42C07D305/14C07D311/30C07D311/40C07D311/62C07H1/08C07H15/18C07H15/203C07J9/00C07J17/005C07J63/008A61K2300/00
Inventor 白乃生杨美琪田梦琪郭森王姗姗王梦旭王天义彭赛男白璐
Owner NORTHWEST UNIV
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