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Separation and extraction method for gentiopicroside in Picrorhiza scrophulariiflora Pennell

A technology of Tibetan Huhuanglian and gentiopicroside, which is applied in the separation/purification of carbonyl compounds, chemical instruments and methods, and the preparation of sugar derivatives, etc. It can solve the problems of not being suitable for the preparation and use of a large number of products, the purity is not high enough, and the use restrictions, etc. problem, to achieve the effect of low cost, large amount of separation, and increase of extraction amount

Inactive Publication Date: 2017-03-29
CHONGQING MEDICAL & PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two main methods for the separation and purification of gentiopicroside: one uses macroporous adsorption resin method, which has a large separation amount, but the purity is not high enough; the other uses high-speed countercurrent chromatography, which has a small amount of sample , not suitable for the preparation of a large number of products, and the equipment is expensive and limited in use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for separating and extracting gentiopicroside from Tibet Huhuanglian, specifically comprises the following steps:

[0024] A. Take the dried rhizome of Coptis chinensis from Tibet, and use 4.05 times the mass of methanol for ultrasonic extraction or reflux extraction for 3 times, each time for 2 hours, at a temperature of 70°C;

[0025] B. Concentrate the extract obtained in step A to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated methanol solution, leave it overnight, evaporate methanol, and use the solution that evaporates methanol successively with n-hexane, ethyl acetate, and n-butanol were extracted 3 times, each time for 40 minutes (i.e. first extracted 3 times with n-hexane (each time 40 minutes), then extracted 3 times with ethyl acetate (each time 40 minutes), Finally use n-butanol to extract 3 times (every 40 minutes));

[0026] C. Concentrate the n-butanol extract to the extract, and dissolve th...

Embodiment 2

[0031] The method for separating and extracting gentiopicroside from Tibet Huhuanglian, specifically comprises the following steps:

[0032] A. Take the dried rhizome of Coptis chinensis from Tibet, and use 3.88 times the mass of methanol for ultrasonic extraction or reflux extraction for 3 times, each time for 2 hours, at a temperature of 50°C;

[0033] B. Concentrate the extract obtained in step A to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated methanol solution, leave it overnight, evaporate methanol, and use the solution that evaporates methanol successively with n-hexane, ethyl acetate, and n-butanol were extracted 3 times, each time for 50 minutes (i.e. first extracted 3 times with n-hexane (50 minutes each time), then extracted 3 times with ethyl acetate (50 minutes each time), Finally use n-butanol to extract 3 times (every 50 minutes));

[0034] C. Concentrate the n-butanol extract to the extract, and dissolve th...

Embodiment 3

[0039] The method for separating and extracting gentiopicroside from Tibet Huhuanglian, specifically comprises the following steps:

[0040] A. Take the dried rhizome of Coptis chinensis from Tibet, and use 3.96 times the mass of methanol for ultrasonic extraction or reflux extraction for 3 times, each time for 2 hours, at a temperature of 60°C;

[0041] B. Concentrate the extract obtained in step A to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated methanol solution, leave it overnight, evaporate methanol, and use the solution that evaporates methanol successively with n-hexane, ethyl acetate, and n-butanol were extracted 3 times, each time for 45 minutes (i.e. first extracted 3 times with n-hexane (each 45 minutes), then extracted 3 times with ethyl acetate (each time 45 minutes), Finally use n-butanol to extract 3 times (each 45 minutes));

[0042] C. Concentrate the n-butanol extract to the extract, and dissolve the extr...

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Abstract

Belonging to the technical field of chemical industry, the invention in particular relates to a separation and extraction method for gentiopicroside in Picrorhiza scrophulariiflora Pennell. The invention specifically employs extraction technique, macroporous resin column chromatography and silica gel column chromatography technique to elute a lot of gentiopicroside individually from a silica gel chromatographic column, thus increasing the extraction amount of gentiopicroside, and the finally obtained gentiopicroside has purity up to 98.7-99.3%, thus greatly improving the purity of gentiopicroside. The method provided by the invention has the advantages of wide application range, convenience and rapidity, low cost, simple operation and large separation amount, thereby being suitable for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the technical field of chemical industry, and in particular relates to a method for separating and extracting gentiopicroside from Tibet's rhizome. Background technique [0002] Tibet Huhuanglian, also known as false Huhuanglian, is the dried rhizome of the perennial herb of the family Scrophulariaceae Huhuanglian. It is bitter in taste and cold in nature. It is an important medicinal material for clearing deficiency and heat. It has the functions of purging fire and detoxifying, and can be used for diseases such as bone steaming, labor heat, jaundice, cold and heat diarrhea, malnutrition in children, spontaneous sweating, night sweats, hemorrhoids, and sores. Studies have shown that the content of gentiopicroside in Tibetan Huhuanglian is relatively high. Gentiopicroside has anti-inflammatory, analgesic, hepatoprotective, and choleretic effects, and can be used to treat rheumatic joint pain, tuberculosis hot flashes, infantil...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08C07C45/78C07C45/79C07C49/225
CPCC07H17/04C07C45/78C07C45/79C07H1/08C07C49/225C07C49/255
Inventor 朱仝飞闫志慧
Owner CHONGQING MEDICAL & PHARMA COLLEGE
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